Background: Rural migrants in China often experience serious residential segregation in destination cities, potentially resulting in limited access to public health education. However, the effect of residential segregation on public health education access remains unexplored. The present paper aims to address three issues. Does residential segregation have significant effects on access to public health education? If it does, what are its potential mechanisms accounting for? Additionally, are any heterogeneity effects differentiated by local duration, migration patterns, migration traits, and regional variations?Methods: The data from the 2014 National Migrants Population Dynamic Monitoring Survey and Logit regression are applied to explore the association between residential segregation and access to public health education. We further use multinomial treatment effects regression to address the endogenous issue. Several Logit models are also used to investigate potential mechanisms and heterogeneous effects.Results :The Logit estimations reveal that rural migrants in segregated neighborhoods are negatively related with lower prevalence of attendance (OR: 0.9200, 95% CI: 0.8500, 0.9958) and online participation of public health education (OR: 0.8709, 95% CI: 0.7893, 0.9609). The negative effects of residential segregation on access to public health education are also drawn in the multinomial treatment effects regressions (attendance model: coefficient: −4.3321, 95% CI: −8.6404, −0.0238; method model: coefficient: −1.6482; 95% CI: −2.6790, −0.6173). The mechanism analysis also demonstrates that residential segregation is negatively associated with the two potential transmission channels: social network formation (OR: 0.6630, 95% CI: 0.6098, 0.7209) and community participation (OR: 0.7880, 95% CI: 0.7106, 0.8737).Conclusion: Residential segregation produces a negative effect on public health education access. Social network and community participation may act as the transmission channel that links residential segregation and access to public health education. Additionally, the effects of residential segregation on public health education are differentiated across regional variations, local duration, migration patterns, and family migration.