Anxiolytic-Like and Antinociceptive Effects of 2(S)-Neoponcirin in Mice

Abstract

Study aims: 2(S)-neopincirin (NEO) is a constituent from of Clinopodium mexicanum, which is used in traditional Mexican herbal medicine for its tranquilizing and analgesic properties. This study investigated the anxiolytic-like, sedative and antinociceptive effects of NEO in several mice models. Material and methods: The anxiolytic-like effect was evaluated in the hole-board (HBT) and Open Field Tests (OFT); sedative effect was evaluated in sleeping time induced by sodium pentobarbital, and its antinociceptive actions were measured in the hot plate test. To evaluate if the GABA receptor could be involved in the anxiolytic-like effect produced by NEO, in independent experiments, the effects produced by co-administration of NEO plus muscimol (MUS) and NEO plus Pitrotoxin (PTX) were evaluated in the HBT. Results: NEO was isolated from Clinopodium mexicanum leaves. The NMR, MS and optic rotation data helped establish its identity as (2S)-5-hydroxy-4′-methoxyflavanone-7-O-{β-glucopyranosyl-(1→6)-β-rhamnoside}. NEO showed an anxiolytic-like effect and was able to counter the nociception induced by a thermal stimulus in a dose-dependent manner. PTX blocked the anxiolytic-like effect of NEO, while MUS was able to enhance it. Conclusions: The findings of present work demonstrated that NEO possesses anxiolytic-like and antinociceptive effects in mice. Such OPEN ACCESSMolecules 2013, 18 7585 effects are not associated with changes in the locomotor activity. These results supported the notion that anxiolytic-like effect of NEO involves the participation of GABAergic system.

Inhibitory effects of Ponciri Fructus on testosterone-induced benign prostatic hyperplasia in rats

“…Ponciri Fructus is a widely used a traditional herbal medicine. It has been reported that three flavonoids (naringin, neoponcirin and poncirin) and three coumarins (umbelliferone, imperatorin and auraptene) isolated from the 70% ethanolic extract of Ponciri fructus have various pharmacological actions including antioxidant [ 27 ], anxiolytic-like [ 28 ], anticancer [ 29 ], and anti-inflammatory effects [ 30 – 32 ]. Therefore, we propose that PFE can have antiproliferative and antioxidant effects based on the various pharmacological activities of the chemical-chemical interactions on the development and progression of lesions in a TP-induced model of BPH.…”

Inhibitory effects of Ponciri Fructus on testosterone-induced benign prostatic hyperplasia in rats

“…It has been reported that three flavonoids (naringin, neoponcirin and poncirin) and three coumarins (umbelliferone, imperatorin and auraptene) isolated from the 70% ethanolic extract of Ponciri fructus have various pharmacological actions including antioxidant [27], anxiolytic-like [28], anticancer [29], and anti-inflammatory effects [30][31][32]. Therefore, we propose that PFE can have antiproliferative and antioxidant effects based on the various pharmacological activities of the chemical-chemical interactions on the development and progression of lesions in a TP-induced model of BPH.…”

Recent Trends in Potential Therapeutic Applications of the Dietary Flavonoid Didymin

“…Among them, didymin is the leading constituent of the complex mixture of flavonoids present in the active extracts of Clinopodium mexicanum . Therefore, in their follow-up study, Cassani et al evaluated the in vivo sedative, anxiolytic-like and antinociceptive effects of didymin which was isolated from leaves of Clinopodium mexicanum [ 41 ]. Didymin showed significant anxiolytic-like activities and was able to against thermal stimuli-induced nociception, and this anxiolytic-like action could be blocked by pitrotoxin but was enhanced by muscimol.…”