ANXA2 Silencing Inhibits Proliferation, Invasion, and Migration in Gastric Cancer Cells

Xie, Rui; Liu, Jia; Yu, Xuefeng; Li, Chunfeng; Wang, Yufeng; Yang, Wei; Hu, Jiahe; Liu, Ping; Liang, Peiqiang; Huang, Xinyan; Wang, Lijuan; Bai, Yuxian; Xue, Yingwei; Zhu, Jiuxin; Fang, Tianyi

doi:10.1155/2019/4035460

Cited by 13 publications

(11 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ANXA2 has recently been linked to the metastasis of several types of cancer, such as prostate [ 27 ], colorectal [ 28 ], and breast cancer [ 29 ]. High expression of ANXA2 promotes the development of GC and is negatively correlated with the clinical prognosis [ 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

EphA2–YES1–ANXA2 pathway promotes gastric cancer progression and metastasis

Mao

Yuan

Cai³

et al. 2021

Oncogene

View full text Add to dashboard Cite

Erythropoietin-producing hepatocellular receptor A2 (EphA2) is a key member of the receptor tyrosine kinase (RTK) family, while YES Proto-Oncogene 1 (YES1) is a non-receptor tyrosine kinase (nRTK) and annexin A2 (ANXA2) belongs to the calcium-dependent phospholipid-binding protein family annexins. Here, we show that EphA2, YES1, and ANXA2 form a signal axis, in which YES1 activated by EphA2 phosphorylates ANXA2 at Tyr24 site, leading to ANXA2 activation and increased ANXA2 nuclear distribution in gastric cancer (GC) cells. Overexpression (OE) of YES1 increases, while knockdown (KD) of YES1 or ANXA2 decreases GC cell invasion and migration in vitro and tumor growth in mouse models. Reexpression of wildtype (WT) rather than mutant ANXA2 (Tyr24F) in ANXA2 knockdown (ANXA2-KD) GC cells restores YES1-induced cell invasion and migration, while neither WT nor mutant ANXA2 (Tyr24F) can restore cell invasion and migration in YES1-KD GC cells. In addition, the activation of EphA2–YES1–ANXA2 pathway is correlated with poor prognosis. Thus, our results establish EphA2–YES1–ANXA2 axis as a novel pathway that drives GC invasion and metastasis, targeting this pathway would be an efficient way for the treatment of GC.

show abstract

Section: Introductionmentioning

confidence: 99%

EphA2–YES1–ANXA2 pathway promotes gastric cancer progression and metastasis

Mao

Yuan

Cai³

et al. 2021

Oncogene

View full text Add to dashboard Cite

show abstract

“…Previous studies have shown that VEGF and IL-6 were highly expressed in GC and promoted the occurrence and development of GC by promoting angiogenesis and maintaining continuous uncontrollable inflammatory response [ 31 ]. Here, we analyzed the relationship between SERPINE1 expression and VEGF and IL-6 to explore the possible mechanism in GC.…”

Section: Resultsmentioning

confidence: 99%

“…Tumor metastasis is the result of many factors, and the process is more complex, including cancer cells entering the blood, invading lymph nodes, transiting through the tumor microenvironment, aggregating and secondary tissues, etc. Cancer cell migration plays an important role in the process of tumor metastasis, but the specific mechanism has not been determined and needs further studied [ 31 ], p. 2.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

SERPINE1 Overexpression Promotes Malignant Progression and Poor Prognosis of Gastric Cancer

Chen¹,

Zhu³

et al. 2022

Journal of Oncology

View full text Add to dashboard Cite

The serine protease inhibitor clade E member 1 (SERPINE1) is a major inhibitor of tissue plasminogen activator and urokinase, and has been implicated in the development and progression of a variety of tumors. In this study, mRNA microarray and TCGA database were used to comprehensively analyze the upregulation of SERPINE1 in gastric cancer (GC) tissues compared with the normal stomach tissues. Kaplan-Meier results confirmed that patients with high SERPINE1 expression exhibited worse overall survival and disease-free survival. In addition, cell proliferation, cell scratches, transwell migration and invasion assay showed that SERPINE1 knockdown inhibited the proliferation, migration and invasion of GC ells. Western blot showed that the expression of VEGF and IL-6 was significantly upregulated after overexpression of SERPINE1. Meanwhile, SERPINE1 was positively correlated with the level of immune infiltration using the online analysis tools TISIDB and TIMER. And SERPINE1 expression increased with the increase of malignancy of GC which were detected by Immunohistochemistry. Finally, tumorigenesis experiments in nude mice further demonstrated that SERPINE1 could promote the occurrence and development of GC, while deletion of SERPINE1 inhibited the progression of GC. In summary, SERPINE1 was highly expressed in GC tissues, and SERPINE1 was helpful for differential diagnosis of pathological grade of gastric mucosal lesions. SERPINE1 might regulate the expression of VEGF and IL-6 through the VEGF signaling pathway and JAK-STAT3 inflammatory signaling pathway, thus ultimately affecting the invasion and migration of GC cells.

show abstract

“…ANXA2, a member of the annexin family, mainly participates in cytoskeleton rearrangement and plasminogen activation [20]. Accumulated evidence has indicated that ANXA2 expressed on the cell membrane of gastric cancer cells was important to maintain the malignancy of cells and becomes a potential prognostic marker in gastric cancer [21][22][23][24]. Overexpression of ANXA2 has been reported in gastric epithelial cells following H. pylori infection, indicating its involvement in H. pylori pathogenesis [25].…”

Section: Biomed Research Internationalmentioning

confidence: 99%

Identification of Functional Interactome of Gastric Cancer Cells with Helicobacter pylori Outer Membrane Protein HpaA by HPLC-MS/MS

Fan

Han

Xiao

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

HpaA as an outer membrane protein of Helicobacter pylori (H. pylori) plays a significant role in the adhesion to the human stomach, but the functional relation between HpaA and gastric epithelial cells is still not clear. To screen the interaction between HpaA and cellular proteins in gastric epithelial cells, the HpaA protein from H. pylori 26695 fused with a tag (6× His) was expressed and purified successfully, the secondary structure was estimated by the Circular Dichroism (CD) spectrum, and the purified recombinant protein was used to perform the pull-down assays with gastric cancer cell lines (AGS and SGC-7901) lysates, respectively. The pull-down proteins were identified by high-performance liquid chromatography tandem mass spectrometry system (HPLC-MS/MS). A total of 9 and 13 proteins related were analyzed from AGS and SGC-7901 cell lysates, respectively. ANXA2 was considered as putative HpaA functional partner discovered from lysates of both cell lines with high score and coverage. It is hypothesized that HpaA may be involved in the biological process of regulation of transcription and nucleic acid metabolism during the adhesion of H. pylori to human gastric epithelial cells, and HpaA-binding proteins also be used as targets for the development of antiadhesion drugs against H. pylori.

show abstract

ANXA2 Silencing Inhibits Proliferation, Invasion, and Migration in Gastric Cancer Cells

Cited by 13 publications

References 28 publications

EphA2–YES1–ANXA2 pathway promotes gastric cancer progression and metastasis

EphA2–YES1–ANXA2 pathway promotes gastric cancer progression and metastasis

SERPINE1 Overexpression Promotes Malignant Progression and Poor Prognosis of Gastric Cancer

Identification of Functional Interactome of Gastric Cancer Cells with Helicobacter pylori Outer Membrane Protein HpaA by HPLC-MS/MS

Contact Info

Product

Resources

About