Antiviral effects of Shuanghuanglian injection powder against influenza A virus H5N1 in vitro and in vivo

Tang, Yuling; Wang, Zhaohua; Huo, Caiyun; Guo, Xiaotong; Yang, Guanghui; Wang, Ming; Tian, Haiyan; Hu, Yanxin; Hong, Daojun

doi:10.1016/j.micpath.2018.06.004

Cited by 35 publications

(18 citation statements)

References 15 publications

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“…Shuang-Huang-Lian (SHL), a combination of JYH, F. suspensa and S. baicalensis, is the most typical formula to explain the traditional use of JYH. As a classic formula, SHL has been used for the treatment of cough, sore throat and fever, acting by dispelling wind, clearing heat and detoxification Tang et al 2018;Tian et al 2018). Till now, SHL has also been used extensively, and its preparations involve granules, oral liquid, injection, etc.…”

Section: No Prescription Namementioning

confidence: 99%

Lonicerae japonicae flos and Lonicerae flos: a systematic review of ethnopharmacology, phytochemistry and pharmacology

et al. 2019

Phytochem Rev

114

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Lonicerae japonicae flos (called Jinyinhua, JYH in Chinese), flowers or flower buds of Lonicera japonica Thunberg, is an extremely used traditional edible-medicinal herb. Pharmacological studies have already proved JYH ideal clinical therapeutic effects on inflammation and infectious diseases and prominent effects on multiple targets in vitro and in vivo, such as pro-inflammatory protein inducible nitric oxide synthase, toll-like receptor 4, interleukin-1 receptor. JYH and Lonicerae flos [called Shanyinhua, SYH in Chinese, flowers or flower buds of Lonicera hypoglauca Miquel, Lonicera confusa De Candolle or Lonicera macrantha (D.Don) Spreng]which belongs to the same family of JYH were once recorded as same herb in multiple versions of Chinese Pharmacopoeia (ChP). However, they were listed as two different herbs in 2005 Edition ChP, leading to endless controversy since they have close proximity on plant species, appearances and functions, together with traditional applications. In the past decades, there has no literature regarding to systematical comparison on the similarity concerning research achievements of the two herbs. This review comprehensively presents similarities and differences between JYH and SYH retrospectively, particularly proposing them the marked differences in botanies, phytochemistry and pharmacological activities which can be used as evidence of separate list of JYH and SYH. Furthermore, deficiencies on present studies have also been discussed so as to further research could use for reference.

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Section: No Prescription Namementioning

confidence: 99%

Lonicerae japonicae flos and Lonicerae flos: a systematic review of ethnopharmacology, phytochemistry and pharmacology

et al. 2019

Phytochem Rev

114

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“…Shuanghuanglian injection can inhibit the in ammation associated with viral encephalitis, and it has been reported that Shuanghuanglian can play anti-in ammatory and antioxidant roles in alveolar macrophages of rats. Shuanghuanglian injection can prevent H5N1 virus infection by inhibiting viral replication and reducing lung injury and can effectively exert anti-HAdV virus effects by inhibiting virus penetration (78)(79). We analyze the effective compounds in Shuanghuanglian traditional Chinese medicine compound, explore the proteins and corresponding genes that they act on, and compare the genes that act with SARS-related genes, and nd that these two types of genes have 151 common gene action targets.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Potential therapeutic value of Shuanghuanglian Chinese medicine based on network pharmacology for coronavirus pneumonia

Xu¹,

Liao²,

Zhang³

et al. 2020

Preprint

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Objective: The interaction network between coronavirus pneumonia and Shuanghuanglian was established to explore the potential therapeutic effect of the active ingredients of Shuanghuanglian on coronary virus pneumonia.Methods: Using the TCMSP database, the effective components of Shuanghuanglian were obtained by screening consistent with oral utilization，drug similarity and blood-brain barrier permeability thresholds, and the drug target prediction was performed.The SARS treatment target mining was performed through the GeneCards database, and the two data sets of therapeutic target and drug target were analyzed and the intersection was screened, and the wayne map was drawn.The intersection genes were used as potential therapeutic targets. Cytoscape 3.6 software was used to build a drug active ingredient-therapeutic target interaction network and analyze the active ingredient-therapeutic target point network Degree parameters to find important active ingredients and targets. Using DAVID and String databases to perform GO,KEGG enrichment analysis and protein interaction analysis on the intersection genes to find out the potential signal pathway of Shuanghuanglian against SARS.Results: 43 effective components against SARS were screened, includingcoptisine、Mandenol、Ethyl linolenate、phytofluene、wogonin、FORSYTHINOL、baicalein、Moslosooflavone、Panicolin, etc.A total of 115 intersection genes with coronavirus pneumonia were screened as important treatment targets: based on the protein interaction network, Shuanghuanglian's therapeutic targets for diseases were significantly enriched in 1763 GO and 133 KEGG signaling pathways; The main action pathways are: responses to steroid hormones and ketones, and development of the reproductive system, responses to lipopolysaccharides, Toxins, responses to bacteria and aging, and effects on epithelial cell proliferation.Conclusion: The active ingredients in Shuanghuanglian Chinese medicine compound can act on the disease-related target of coronavirus pneumonia and have potential therapeutic effects on coronavirus pneumonia.

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“…The expression pattern of sialic acid receptors of cells was detected as previously described (Meng et al, 2016 ; Tang et al, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

H5N1 Influenza a Virus Replicates Productively in Pancreatic Cells and Induces Apoptosis and Pro-Inflammatory Cytokine Response

Huo

Xiao

Zhang

et al. 2018

Front. Cell. Infect. Microbiol.

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The inflammatory response and apoptosis have been proved to have a crucial role in the pathogenesis of the influenza A virus (IAV). Previous studies indicated that while IAV commonly causes pancreatitis and pancreatic damage in naturally and experimentally infected animals, the molecular mechanisms of the pathogenesis of IAV infection are less reported. In the present study, we showed for the first time that both avian-like (α-2,3-linked) and human-like (α-2,6-linked) sialic acid (SA) receptors were expressed by the mouse pancreatic cancer cell line PAN02 and the human pancreatic cancer cell line PANC-1. Using growth kinetics experiments, we also showed that PAN02 and PANC-1 cells supported the productive replication of the H5N1 highly pathogenic avian influenza while exhibited the limited replication of IAV subtypes H1N1 and H7N2 in vitro. The in vivo infection of H5N1 in pancreatic cells was confirmed by the histopathological and immunohistochemical staining of pancreas tissue from mice. Other than H1N1 and H7N2, severe damage and extensive positive signals were observed in pancreas of H5N1 infected mice. All three virus subtypes induced apoptosis but also triggered the infected PAN02 and PANC-1 cells to release pro-inflammatory cytokines and chemokines including interferon (IFN)-α, IFN-β, IFN-γ, chemokine (C-C motif) ligand 2 (CCL2), tumor necrosis factor (TNF)-α, and interleukin (IL)-6. Notably, the subtypes of H5N1 could significantly upregulate these cytokines and chemokines in both two cells when compared with H1N1 and H7N2. The present data provide further understanding of the pathogenesis of H5N1 IAV in pancreatic cells derived from humans and mammals and may also benefit the development of new treatment against H5N1 influenza virus infection.

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Antiviral effects of Shuanghuanglian injection powder against influenza A virus H5N1 in vitro and in vivo

Cited by 35 publications

References 15 publications

Lonicerae japonicae flos and Lonicerae flos: a systematic review of ethnopharmacology, phytochemistry and pharmacology

Lonicerae japonicae flos and Lonicerae flos: a systematic review of ethnopharmacology, phytochemistry and pharmacology

Investigation of Potential therapeutic value of Shuanghuanglian Chinese medicine based on network pharmacology for coronavirus pneumonia

H5N1 Influenza a Virus Replicates Productively in Pancreatic Cells and Induces Apoptosis and Pro-Inflammatory Cytokine Response

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