Antiviral effect of lithium chloride on infection of cells by porcine parvovirus

Chen, Ye; Yan, Huichao; Zheng, Hao; Shi, Yuhua; Sun, Lichao; Wang, Chong; Sun, Jingchen

doi:10.1007/s00705-015-2352-z

Cited by 21 publications

(19 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There are several studies exploring the roles of LiCl on virus infection and suggesting that LiCl inhibit certain virus replication, such as transmissible gastroenteritis coronavirus, HIV, canine parvovirus, PRRSV and so on [8][9][10][11][12][13][14][15]. In this study, we demonstrate that LiCl can also restrict CVB3 infection in vivo and in vitro.…”

Section: Discussionsupporting

confidence: 54%

“…Subsequent to changing the medium and PBS-wash, cells were cultured at 37°C for 24 hour and were analyzed by Q-PCR and Western blot. The results showed that the viral RNA level and VP-1 expression were not altered after 8 treatment with LiCl ( Fig. 3A-B).…”

Section: Licl Does Not Affect Cvb3 Attachment and Entrymentioning

confidence: 89%

“…So LiCl has been explored for treating diabetes, Alzheimer's disease and ovarian cancer, as well as antimicrobial infection [7]. Prior investigations have also revealed that LiCl exhibits antiviral effects on certain viruses, e.g., coronavirus, herpes simplex, infectious bronchitis virus, porcine reproductive and respiratory syndrome (PRRS) and feline calicivirus [8][9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Lithium chloride confers protection against viral myocarditis via suppression of coxsackievirus B3 virus replication

Zhao

Yan

Wang

et al. 2020

Microbial Pathogenesis

View full text Add to dashboard Cite

Lithium chloride confers protection against viral myocarditis via suppression of coxsackievirus B3 virus replication, Microbial Pathogenesis, https://doi. Highlights Lithium chloride inhibits coxsackievirus B3 virus replication in vitro and in vivo.Lithium chloride inhibits CVB3 replication via inhibiting virus-induced cell apoptosis.LiCl treatment in vivo obviously alleviated CVB3-induced acute myocarditis. 3 Abstract Viral myocarditis (VMC) is a type of inflammation affecting myocardial cells caused by viral infection and has been an important cause of dilated cardiomyopathy (DCM) worldwide. Type B3 coxsackievirus (CVB3), a non-enveloped positive-strand RNA virus of the Enterovirus genus, is one of most common agent of viral myocarditis. Till now, effective treatments for VMC are lacking due to lack of drugs or vaccine. Lithium chloride (LiCl) is applied in the clinical management of manic depressive disorders. Accumulating evidence have demonstrated that LiCl, also as an effective antiviral drug, exhibited antiviral effects for specific viruses. However, there are few reports of evaluating LiCl's antiviral effect in mice model. Here, we investigated the inhibitory influence of LiCl on the CVB3 replication in vitro and in vivo and the development of CVB3-induced VMC. We found that LiCl significantly suppressed CVB3 replication in HeLa via inhibiting virus-induced cell apoptosis. Moreover, LiCl treatment in vivo obviously inhibited virus replication within the myocardium and alleviated CVB3-induced acute myocarditis. Collectively, our data demonstrated that LiCl inhibited CVB3 replication and negatively regulated virus-triggered inflammatory responses.Our finding further expands the antiviral targets of LiCl and provides an alternative agent for viral myocarditis. Conflict of interestThe authors declare no conflict of interest. Ethical approvalThe animal experiments were performed in accordance with Soochow University institutional guidelines, and the study was approved by the Ethics Committee of Soochow University in written form. Euthanasia of mice was performed by carbon dioxide inhalation with minimum fear, anxiety and pain. HighlightsLithium chloride inhibits coxsackievirus B3 virus replication in vitro and in vivo.

show abstract

Section: Discussionsupporting

confidence: 54%

Section: Licl Does Not Affect Cvb3 Attachment and Entrymentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lithium chloride confers protection against viral myocarditis via suppression of coxsackievirus B3 virus replication

Zhao

Yan

Wang

et al. 2020

Microbial Pathogenesis

View full text Add to dashboard Cite

show abstract

“…The onset of respiratory symptoms should lead to a reassessment of the risk-benefit balance of these treatments. Further to this, several publications report the in vitro efficacy of certain psychotropic drugs on viruses similar to coronaviruses: in particular lithium and chlorpromazine (with greater efficacy than chloroquine), but this exclusively preclinical data to date does not make it possible to validate the clinical use of these treatments (25,26).…”

Section: Individual Vulnerability Of Psychiatric Patients To Coronavirusmentioning

confidence: 99%

Ensuring mental health care during the SARS-CoV-2 epidemic in France: A narrative review

et al. 2020

View full text Add to dashboard Cite

Objective: The lack of resources and coordination to face the coronavirus epidemic raises concerns for the health of patients with mental disorders in a country where we still have memories of the dramatic experience of famine in psychiatric hospitals during the Second World War. This article aims to propose guidance to ensure mental health care during the SARS-CoV epidemic in France. Methods: The authors performed a narrative review identifying relevant results in the scientific and medical literature and in local initiatives in France. Results : We identified four types of major vulnerabilities among patients with mental disorders during this pandemic: 1) medical comorbidities that are more frequently found among patients with mental disorders (cardiovascular and pulmonary pathologies, diabetes, obesity, etc.) which are risk factors for severe covid-19 infection; 2) age (the elderly form the population most vulnerable to the coronavirus); 3) cognitive and behavioural disorders, which can hamper compliance with confinement and hygiene measures and finally and 4) psychosocial vulnerability as a result of stigmatization and/or socio-economic difficulties. Furthermore, the mental health healthcare system is more vulnerable than other healthcare systems. Current government plans are poorly suited to psychiatric establishments in a context of major shortages of organizational, material and human resources. In addition, a certain number of structural aspects make the psychiatric institution particularly vulnerable: many beds have been closed, wards have high densities of patients, mental health community facilities are closed, and medical teams are understaffed and poorly trained to face infectious diseases. There are also major issues when referring patients with acute mental disorders to intensive care units. To maintain the continuity of psychiatric care in this pandemic situation, several directions can be considered, in particular with the creation of "COVID+ units". These units are under the dual supervision of a psychiatrist and an internist / infectious disease specialist; all new entrants are placed in quarantine for 14 days; the nursing staff receives specific training, daily medical checkups and close psychological support. Family visits are prohibited and replaced by videoconference. At the end of hospitalization, in particular for the population of patients in compulsory ambulatory care situations, specific case-management are organized with the possibility of home visits, in order to support patients when they get back home and to help them cope with the experience of confinement, which is liable to induce recurrences of mental disorders. The total or partial closure of community mental health facilities is particularly disturbing for patients, but a regular follow-up is possible with telemedicine and should include the monitoring of suicide risk and psycho-education strategies;developing support platforms could also be very helpful in this context. Private practice psychiatrists also have a crucial role...

show abstract

“…Antiviral effects of LiCl have been extensively investigated. LiCl can inhibit the infection of some DNA and RNA viruses, such as herpes simplex virus (Skinner et al, 1980), canine parvovirus (Zhou et al, 2015), porcine parvovirus (Chen et al, 2015), pseudorabies herpesvirus (Sui et al, 2010), porcine reproductive and respiratory syndrome virus (Hao et al, 2015), feline calicivirus (Wu et al, 2015), infectious bronchitis virus, an avian coronavirus (Li et al, 2009) and transmissible gastroenteritis virus (TGEV), a porcine coronavirus (Ren et al, 2011). Both PEDV and TGEV are classified into group I of the genus Alphacoroavirus.…”

Section: Introductionmentioning

confidence: 99%

Antiviral effect of lithium chloride on porcine epidemic diarrhea virus in vitro

Gao

et al. 2018

Research in Veterinary Science

View full text Add to dashboard Cite

Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family, causes acute diarrhea, vomiting, dehydration, and high mortality rates in neonatal piglets. Severe outbreaks of PEDV variants have re-emerged in Asia and North America since 2010, causing tremendous economic losses to the swine industry. The lack of effective therapeutic treatment promotes the research for new antivirals. Lithium chloride (LiCl) has been reported as a potential antiviral drug for certain viruses. In this study, the antiviral effect of LiCl on PEDV in Vero cells was evaluated. Real-time quantitative PCR and indirect immunofluorescence assay indicated that LiCl effectively inhibited the entry and replication of PEDV in Vero cells. The expression of viral RNA and protein of PEDV in Vero cells was suppressed in a dose-dependent manner by LiCl. Moreover, addition of LiCl inhibited both early and late cell apoptosis induced by PEDV. Our data implied that LiCl could be a potential antiviral drug against PEDV infection. Further studies are required to explore the antiviral effect of lithium chloride on PEDV infection in vivo.

show abstract

Antiviral effect of lithium chloride on infection of cells by porcine parvovirus

Cited by 21 publications

References 26 publications

Lithium chloride confers protection against viral myocarditis via suppression of coxsackievirus B3 virus replication

Lithium chloride confers protection against viral myocarditis via suppression of coxsackievirus B3 virus replication

Ensuring mental health care during the SARS-CoV-2 epidemic in France: A narrative review

Antiviral effect of lithium chloride on porcine epidemic diarrhea virus in vitro

Contact Info

Product

Resources

About