Antiviral Effect of Brassinosteroids against Herpes Virus and Arenaviruses

Wachsman, Mónica B.; López, Elsa Mf; Ramírez, Javier A.; Galagovsky, Lydia R.; Coto, Celia E.

doi:10.1177/095632020001100107

Cited by 84 publications

(62 citation statements)

References 22 publications

(22 reference statements)

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“…Among the tested compounds, 6b displayed a signifi cant antiviral activity against HSV-1 multiplication on confl uent or growing Vero cells [8,9] . In addition, the derivative did not inactivate viral infectivity during in vitro incubation [7] . Time of addition experiments showed that 6b mainly affects a late event of the virus growth cycle.…”

Section: Introductionmentioning

confidence: 91%

“…Brassinosteroids are a group of steroids that appear to be ubiquitous in plants and are essential for normal plant growth and development. Brassinosteroid-like structures have been shown to exert antiviral activity against HSV timidine kinase (tk)+ and tk-strains [7,8] and some other viruses [7,9,10] . Among the tested compounds, 6b displayed a signifi cant antiviral activity against HSV-1 multiplication on confl uent or growing Vero cells [8,9] .…”

Section: Introductionmentioning

confidence: 99%

“…Neither adsorption nor internalization of virus particles was the target of the inhibitory action. We also determined that 6b principally inhibits late HSV protein synthesis [7,11] .…”

Section: Introductionmentioning

confidence: 99%

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Synergistic in vitro Interactions between (22S,23S)-3β-Bromo-5α,22,23-Trihydroxystigmastan-6-one and Acyclovir or Foscarnet against Herpes simplex Virus Type 1

Talarico¹,

Castilla²,

Ramírez

et al. 2005

Chemotherapy

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The replication of herpes simplex virus (HSV) type 1 in Vero cells is inhibited in the presence of (22S,23S)-3β-bromo-5α,22,23-trihydroxystigmastan-6-one (6b), a synthetic brassinosteroid derivative. Since a late step of virus multiplication is hindered by 6b, we performed studies of drug-drug combination with acyclovir (ACV) and foscarnet (FOS). It was determined that 6b would act synergistically with low concentrations of ACV and moderate concentrations of FOS against HSV. The best drug combination tested in this study resulted in an increase of 29.3 and 47.2% in antiviral activity for ACV (0.036 µM) and FOS (37.5 µM) in the presence of 14.8 and 6.9 µM of 6b, respectively.

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Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Synergistic in vitro Interactions between (22S,23S)-3β-Bromo-5α,22,23-Trihydroxystigmastan-6-one and Acyclovir or Foscarnet against Herpes simplex Virus Type 1

Talarico¹,

Castilla²,

Ramírez

et al. 2005

Chemotherapy

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“…The BR analogs 28-homocastasterone and EBL inhibit cell growth in a dose-dependent manner in breast and prostate cancer cell lines without affecting the normal nontumor cell growth, suggesting that BRs may be promising leads as potential anticancer drugs (Malíková et al, 2008;Steigerová et al, 2010). Furthermore, BR analogs have been reported to have antiviral activities (Wachsman et al, 2000(Wachsman et al, , 2002Castilla et al, 2005;Romanutti et al, 2007).…”

Section: Nutritional Quality Of Crops and Medicinal Application Of Phmentioning

confidence: 99%

Boosting Crop Yields with Plant Steroids

2012

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Plant sterols and steroid hormones, the brassinosteroids (BRs), are compounds that exert a wide range of biological activities. They are essential for plant growth, reproduction, and responses to various abiotic and biotic stresses. Given the importance of sterols and BRs in these processes, engineering their biosynthetic and signaling pathways offers exciting potentials for enhancing crop yield. In this review, we focus on how alterations in components of sterol and BR metabolism and signaling or application of exogenous steroids and steroid inhibitors affect traits of agronomic importance. We also discuss areas for future research and identify the fine-tuning modulation of endogenous BR content as a promising strategy for crop improvement.

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“…Other studies by Wachsman et al [41,42] found that the brassinosteroid and its derivatives inhibited the in vitro replication of herpes simplex type 1 (HSV-1) thymidine kinase (TK) + and TK-strains. Where, TC50: the concentration which exhibited 50 % cytotoxicity; IC50 : the half maximal inhibitory concentration.…”

Section: Antiviral Activitymentioning

confidence: 99%

Sterols Bioactivity of Ruta Graveolens L. And Murraya Paniculata L.

Baker

Ibrahim

Kandeil

et al. 2017

Int J Pharm Pharm Sci

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Objective: Ruta graveolens L. (R. graveolens) and Murraya paniculata L. (M. paniculata) are medicinal plants belonging to Rutaceae family have many uses in traditional medicine. The aim of the present study was to investigate sterols bioactivity of the two Rutaceae plant leaves.Methods: Sterols of the two Rutaceae plant leaves were identified using GC/MS. The antioxidant activities of the sterols of these herbs were evaluated by three different methods; free radical scavenging using 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 1,1-Diphenyl-2-picryl-hydrazyl ( Results: GC/MS assay showed β-Sitosterol (36%) as the most abundant sterols of R. graveolens followed by stigmasterol (18%), while stigmasterol (25.2%) was the most abundant one of M. paniculata steroids. The anti-inflammatory potential of R. graveolens steroids was significantly higher than that of diclofenac sodium (standard drug). M. paniculata sterols have higher antiviral activity (IC DPPH) and total antioxidant activity. The anticancer activity of the sterols was determined by MTT assay against colorectal cancer HCT116, breast cancer MCF7, liver cancer HepG2 and lung cancer A549 cell lines. Anti-inflammatory activity was evaluated using albumin denaturation assay and antiviral activities against H5N1 virus were carried out using plaque reduction assay. 50= 0.15 of µg/ml) than R. graveolens sterols (IC50 Conclusion:The current study suggests that the sterols of M. paniculata have more anti-viral activity than R. graveolens sterols which showed more anticancer and anti-inflammatory activities. = 7.8 of µg/ml). The sterols of R. graveolens showed anticancer activity against MCF7 and A549 cells with inhibition 84.3 and 81%, at 100 µg/ml respectively. While M. paniculata sterols showed 77.3% inhibition against A549 cells.

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Antiviral Effect of Brassinosteroids against Herpes Virus and Arenaviruses

Cited by 84 publications

References 22 publications

Synergistic in vitro Interactions between (22S,23S)-3β-Bromo-5α,22,23-Trihydroxystigmastan-6-one and Acyclovir or Foscarnet against Herpes simplex Virus Type 1

Synergistic in vitro Interactions between (22S,23S)-3β-Bromo-5α,22,23-Trihydroxystigmastan-6-one and Acyclovir or Foscarnet against Herpes simplex Virus Type 1

Boosting Crop Yields with Plant Steroids

Sterols Bioactivity of Ruta Graveolens L. And Murraya Paniculata L.

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