Sterols Bioactivity of Ruta Graveolens L. And Murraya Paniculata L.

Abstract

Objective: Ruta graveolens L. (R. graveolens) and Murraya paniculata L. (M. paniculata) are medicinal plants belonging to Rutaceae family have many uses in traditional medicine. The aim of the present study was to investigate sterols bioactivity of the two Rutaceae plant leaves.Methods: Sterols of the two Rutaceae plant leaves were identified using GC/MS. The antioxidant activities of the sterols of these herbs were evaluated by three different methods; free radical scavenging using 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 1,1-Diphenyl-2-picryl-hydrazyl ( Results: GC/MS assay showed β-Sitosterol (36%) as the most abundant sterols of R. graveolens followed by stigmasterol (18%), while stigmasterol (25.2%) was the most abundant one of M. paniculata steroids. The anti-inflammatory potential of R. graveolens steroids was significantly higher than that of diclofenac sodium (standard drug). M. paniculata sterols have higher antiviral activity (IC DPPH) and total antioxidant activity. The anticancer activity of the sterols was determined by MTT assay against colorectal cancer HCT116, breast cancer MCF7, liver cancer HepG2 and lung cancer A549 cell lines. Anti-inflammatory activity was evaluated using albumin denaturation assay and antiviral activities against H5N1 virus were carried out using plaque reduction assay. 50= 0.15 of µg/ml) than R. graveolens sterols (IC50 Conclusion:The current study suggests that the sterols of M. paniculata have more anti-viral activity than R. graveolens sterols which showed more anticancer and anti-inflammatory activities. = 7.8 of µg/ml). The sterols of R. graveolens showed anticancer activity against MCF7 and A549 cells with inhibition 84.3 and 81%, at 100 µg/ml respectively. While M. paniculata sterols showed 77.3% inhibition against A549 cells.

Evaluation of cell viability and nitric oxide release after treatment of Hepatocellular Carcinoma cells (HepG2) with Ruta graveolens

“…Among the different bioactive compounds present in this plant, phytosterols are known to present anticancer and dose-dependent anti-inflammatory activity. Baker et al proved the action of these substances present in R. graveolens extracts against colorectal cancer, breast cancer, lung cancer, and hepatocellular carcinoma tumor cells (HepG2) 9 . In this way, the effects of the extract of this plant have already been showed, now our study evaluated the cell-based effects of the homeopathic medicine and presented the cytotoxic mechanism against liver cancer cells.…”

“…To mitigate the cytotoxic effects and use as supportive care treatments, homeopathy has been frequently considered. Its use in oncology as supportive care is relatively recent 9 , with in vitro and in vivo publications being published and beginning to demonstrate the potential of homeopathic dilutions affecting cancer cells 7 . Therefore, there are still concerns about the way of action of homeopathic medicines in cancer cells and this study aimed to evaluate the effect of the homeopathic medicine Ruta D35 in human Hepatocellular Carcinoma cells (HepG2) through in vitro cytotoxicity tests.…”

Diversity in Phytochemical Composition and Medicinal Value of Murraya paniculata

“…In a study, it has been reported that sterols from the leaves of M. paniculata inhibited the A549 cancer cells by 77.3 percent. [35] So, the further research work could be done to explore M. paniculata for the treatment of cancers.…”

A Brief Review on Murraya paniculata (Orange Jasmine): pharmacognosy, phytochemistry and ethanomedicinal uses

“…The isolated sterol from MP leaves exhibited antitumor activity against cancer cell lines. The cytotoxic actions of sterols were determined by an MTT assay [48]. In one investigation, the cytotoxic effect of ethyl acetate extracts of MP leaves was investigated on human gingival fibroblasts and monocytes [49].…”