Antiviral activity of uridine 5′-diphosphate glucose analogues against some enveloped viruses in cell culture

Gil-Fernández, G.; Pérez, Sara; Vilas, Pilar; Pérez, Concepción; Heras, F. G. De Las; Gancedo, A. García

doi:10.1016/s0166-3542(87)80007-4

Cited by 15 publications

(5 citation statements)

References 14 publications

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“…This in turn, leads to increased UDPG levels and ultimately P2Y14 expression in macrophages 140 . Gilfernandez and colleagues 141 completely abolished the replication of HSV-2 and African swine fever virus using a uridine 5'-diphosphate glucose analogues at 100 and 150 µg/ml, respectively. These all suggest a key role of P2Y14 in MD and potentially during recruitment of macrophages or other immune cells to the lungs.…”

Section: Resultsmentioning

confidence: 98%

Purinergic signaling during Marek’s disease in chickens

Akbar

Fasick

Ponnuraj

et al. 2023

Sci Rep

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Purinergic receptors (PRs) have been reported as potential therapeutic targets for many viral infections including herpesviruses, which urges the investigation into their role in Marek’s disease (MD), a herpesvirus induced cancer in chickens that is an important pathogen for the poultry industry. MD is caused by MD virus (MDV) that has a similar viral life cycle as human varicella zoster virus in that it is shed from infected epithelial skin cells and enters the host through the respiratory route. In this report, PR responses during natural MDV infection and disease progression was examined in MD-resistant white Leghorns (WL) and MD-susceptible Pure Columbian (PC) chickens during natural infection. Whole lung lavage cells (WLLC) and liver tissue samples were collected from chickens infected but showing no clinical signs of MD (Infected) or presenting with clinical disease (Diseased). RNA was extracted followed by RT-qPCR analysis with gene specific primers against members of the P1, P2X, and P2Y PR families. Differential expression (p < 0.05) was observed in breed and disease conditions. Some PRs showed tissue specific expression (P1A1, P2X1, and P2X6 in WLLC) whereas others responded to MDV infection only in MD-susceptible (PC) chickens (P1A2A, P2X1, P2X5, P2X7). P2Y PRs had differential expression in both chicken lines in response to MDV infection and MD progression. This study is the first to our knowledge to examine PR responses during MDV infection and disease progression. These results suggest PR signaling may an important area of research for MDV replication and MD.

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Section: Resultsmentioning

confidence: 98%

Purinergic signaling during Marek’s disease in chickens

Akbar

Fasick

Ponnuraj

et al. 2023

Sci Rep

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“…Moreover, the presence of hexose, a five-atom bridge (diphosphate-like), and uridine were essential for their antiviral effects . In another work, the same compound possessed in vitro antiviral activity against other enveloped viruses as well …”

Section: Potential Glycosylation Inhibitors For Sars-cov-2mentioning

confidence: 93%

“…67 In another work, the same compound possessed in vitro antiviral activity against other enveloped viruses as well. 68 5.3.7. Chloroquine.…”

Section: 4-dideoxy-14-imino-d-mannitol (Dim)mentioning

confidence: 99%

Exploring the Potential of Chemical Inhibitors for Targeting Post-translational Glycosylation of Coronavirus (SARS-CoV-2)

et al. 2022

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The Spike (S) protein of SARS-CoV-2 expressed on the viral cell surface is of particular importance as it facilitates viral entry into the host cells. The S protein is heavily glycosylated with 22 N-glycosylation sites and a few N-glycosylation sites. During the viral surface protein synthesis via the host ribosomal machinery, glycosylation is an essential step in post-translational modifications (PTMs) and consequently vital for its life cycle, structure, immune evasion, and cell infection. Interestingly, the S protein of SARS-CoV-2 and the host receptor protein, ACE2, are also extensively glycosylated and these surface glycans are critical for the viral–host cell interaction for viral entry. The glycosylation pathway of both virus (hijacked from the host biosynthetic machinery) and target cells crucially affect SARS-CoV-2 infection at different levels. For example, the glycosaminoglycans (GAGs) of host cells serve as a cofactor as they interact with the receptor-binding domain (RBD) of S-glycoprotein and play a protective role in host immune evasion via masking the viral peptide epitopes. Hence, the post-translational glycan biosynthesis, processing, and transport events could be potential targets for developing therapeutic drugs and vaccines. Especially, inhibition of the N-glycan biosynthesis pathway amplifies S protein proteolysis and, thus, blocks viral entry. The chemical inhibitors of SARS-CoV-2 glycosylation could be evaluated for Covid-19. In this review, we discuss the current status of the chemical inhibitors (both natural and synthetically designed inhibitors) of viral glycosylation for Covid-19 and provide a future perspective. It could be an important strategy in targeting the various emerging SARS-CoV-2 variants of concern (VOCs), as these inhibitors are postulated to aid in reducing the viral load as well as infectivity.

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“…The hydrophobic interaction provided by the protecting groups on both sugar and nucleoside moieties may also affect the inhibition activity [61]. For instance, 2 with a benzyl group protected glucose was a better inhibitor against certain viruses [62]. C-Glycosides, 3 and 4, which resemble the natural substrates with an extra methylene group between glycosidic oxygen and anomeric carbon [63], were designed as inhibitors because they should be incapable of glycosyl transfer.…”

Section: C-glycoside Based Inhibitorsmentioning

confidence: 99%

C-Glycosides and Aza-C-Glycosides as Potential Glycosidase and Glycosyltransferase Inhibitors

Zou¹

2005

CTMC

144

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Glycosylation as one of most important post-translational modification of gene products is often critical to specific cellular biological functions. Since elevated glycoprocessing enzyme activities have been implicated in the development of various diseases including cancer metastasis, glycosidases and glycosyltransferases are considered as therapeutic targets. Azasugars, the first generation of enzyme inhibitors, have been extensively investigated and two azasugar-based drugs (Miglitol and Miglustat) have been approved. Aza-C-glycosides, molecules with an azasugar core and various C-aglycons attached at the pseudo anomeric center, have the potential to become the second-generation inhibitors with improved specificity and membrane permeability. In this review, C-glycosides, aza-C-glycosides, and aza-C-disaccharides are introduced as glycoprocessing enzyme inhibitors. The synthetic approaches toward those molecules are described based on the key reactions, which include reductive amination, nucleophilic ring opening of epoxides, nucleophilic addition to imines (C=N), and hetero-Michael additions. Aza-C-glycoside-based libraries are also described for the discovery of promising second-generation inhibitors.

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Antiviral activity of uridine 5′-diphosphate glucose analogues against some enveloped viruses in cell culture

Cited by 15 publications

References 14 publications

Purinergic signaling during Marek’s disease in chickens

Purinergic signaling during Marek’s disease in chickens

Exploring the Potential of Chemical Inhibitors for Targeting Post-translational Glycosylation of Coronavirus (SARS-CoV-2)

C-Glycosides and Aza-C-Glycosides as Potential Glycosidase and Glycosyltransferase Inhibitors

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