Antiviral activity of raoulic acid from Raoulia australis against Picornaviruses

Choi, Hwa-Jung; Lim, Chi-Hwan; Song, Jae-Hyoung; Baek, Seung‐Hwa; Kwon, Dur Han

doi:10.1016/j.phymed.2008.10.012

Cited by 51 publications

(46 citation statements)

References 10 publications

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“…A stock solution of IAV subtype A/ShanTou/169/06 (H1N1) was diluted with Dulbecco's modified Eagle's medium (DMEM) E0 containing 2.5 g/ml trypsin and 3.2% bovine serum albumin (BSA) in serial dilutions and then incubated with MDCK cells for 48 h; the 50% tissue culture infective dose (TCID 50 ) was calculated according to the method of Reed and Muench. Antiviral activities were evaluated by the SRB method using cytopathic effect (CPE) reduction (25). Briefly, MDCK cells (1 ϫ 10 4 cells/well) were seeded in 96-well plates for 24 h. Exactly 0.09 ml of virus suspension (50 TCID 50 ) and 0.01 ml medium containing various concentrations of test compounds were added to the wells.…”

Section: Methodsmentioning

confidence: 99%

Identification of 23-( S )-2-Amino-3-Phenylpropanoyl-Silybin as an Antiviral Agent for Influenza A Virus Infection In Vitro and In Vivo

Dai

et al. 2013

Antimicrob Agents Chemother

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It has been reported that autophagy is involved in the replication of many viruses. In this study, we screened 89 medicinal plants, using an assay based on the inhibition of the formation of the Atg12-Atg5/Atg16 heterotrimer, an important regulator of autophagy, and selected Silybum marianum L. for further study. An antiviral assay indicated that silybin (S0), the major active compound of S. marianum L., can inhibit influenza A virus (IAV) infection. We later synthesized 5 silybin derivatives (S1 through S5) and found that 23-(S)-2-amino-3-phenylpropanoyl-silybin (S3) had the best activity. When we compared the polarities of the substituent groups, we found that the hydrophobicity of the substituent groups was positively correlated with their activities. We further studied the mechanisms of action of these compounds and determined that S0 and S3 also inhibited both the formation of the Atg12-Atg5/Atg16 heterotrimer and the elevated autophagy induced by IAV infection. In addition, we found that S0 and S3 could inhibit several components induced by IAV infection, including oxidative stress, the activation of extracellular signal-regulated kinase (ERK)/p38 mitogen-activated protein kinase (MAPK) and IB kinase (IKK) pathways, and the expression of autophagic genes, especially Atg7 and Atg3. All of these components have been reported to be related to the formation of the Atg12-Atg5/Atg16 heterotrimer, which might validate our screening strategy. Finally, we demonstrated that S3 can significantly reduce influenza virus replication and the associated mortality in infected mice. In conclusion, we identified 23-(S)-2-amino-3-phenylpropanoyl-silybin as a promising inhibitor of IAV infection.

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Section: Methodsmentioning

confidence: 99%

Identification of 23-( S )-2-Amino-3-Phenylpropanoyl-Silybin as an Antiviral Agent for Influenza A Virus Infection In Vitro and In Vivo

Dai

et al. 2013

Antimicrob Agents Chemother

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“…Meliacine also inhibits the low-pH-induced fusion of infected cells and interferes with the release of new particles (13). A study by Choi et al (14) similarly showed that raoulic acid from Raoulia australis has antiviral activity against picornaviruses.…”

Section: Discussionmentioning

confidence: 98%

Antiviral Activity of Alhagi maurorum Medik’s Methanolic Extract on Foot and Mouth Disease Virus (FMDV) in Cell Cultures

Shakiba

Rezatofighi

Nejad

et al. 2016

Jundishapur J Nat Pharm Prod

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“…Raoulic acid (36), purified from a whole-plant extract of a New Zealand plant, Raoulia australis, was tested for antiviral activity in Vero cells and inhibited EV71 with an EC50 of less than 0.1 µg/mL and a CC50 of more than 65 µg/mL, giving it a therapeutic index >650 [31].…”

Section: Sesterterpenoidsmentioning

confidence: 99%

Anti-Enterovirus 71 Agents of Natural Products

Wang

et al. 2015

Molecules

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Antiviral activity of raoulic acid from Raoulia australis against Picornaviruses

Cited by 51 publications

References 10 publications

Identification of 23-( S )-2-Amino-3-Phenylpropanoyl-Silybin as an Antiviral Agent for Influenza A Virus Infection In Vitro and In Vivo

Identification of 23-( S )-2-Amino-3-Phenylpropanoyl-Silybin as an Antiviral Agent for Influenza A Virus Infection In Vitro and In Vivo

Antiviral Activity of Alhagi maurorum Medik’s Methanolic Extract on Foot and Mouth Disease Virus (FMDV) in Cell Cultures

Anti-Enterovirus 71 Agents of Natural Products

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