Antiviral activity of Faramea bahiensis leaves on dengue virus type-2 and characterization of a new antiviral flavanone glycoside

Antiviral Activity of Faramea hyacinthina and Faramea truncata Leaves on Dengue Virus Type‐2 and Their Major Compounds

“…The HPLC‐DAD profiles of these bioactive fractions showed a major compound with similar retention time to the antiviral flavanone glycoside ( 1 ) previously isolated from F . bahiensis ( Figures and ). In order to isolate this compound and to obtain enriched fractions of other constituents, a SPE method was developed.…”

“…These sub‐fractions were also assayed for in vitro cytotoxicity. All of them were non‐cytotoxic but unable to protect the HepG2 against the virus (non‐cytoprotective) (Figure S3) thus showing a change in the activity profile compared to the hydromethanolic fractions and to the bioactive flavanone ( 1 ) . The mobile phase composition range for the elution of the compounds of these less bioactive sub‐fractions varied between 10% and 20% MeCN.…”

“…Based on these data and aiming to increase the knowledge of the chemical constitution of Faramea spp., RP‐CC was performed with these sub‐fractions allowing to isolate the new compounds 2a / 2b from F . hyacinthina and the known compounds naringenin‐7‐ O ‐ β ‐ d ‐apiofuranosyl‐(1→6)‐ β ‐ d ‐glucopyranoside ( 3 ) from both species; rutin ( 4 ), quercetin‐4′‐ O ‐ β ‐ d ‐glucopyranosyl‐3‐ O ‐rutinoside ( 5 ) from F . hyacinthina and kaempferol‐3‐ O ‐rutinoside ( 6 ), erythroxyloside A ( 7 ), asperuloside ( 8 ) from F .…”

“…The same process when applied to the anomeric proton signal set at δ (H) 4.82 – 4.88 ( m ) revealed two doublet s at δ (H) 4.84 ( J = 7.3 Hz) and 4.85 ( J = 7.4 Hz) and other two doublet s at δ (H) 4.86 ( J = 2.7 Hz) and δ (H) 4.87 ( J = 2.4 Hz) ( Figure ). The complementary analysis involving the 1 H, 1 H correlations in the COSY spectrum of the signals at δ (H) 4.86/4.87 ppm with the two signal sets at δ (H) 3.77 ( d , J = 2.7)/3.80 ( d , J = 2.7) together with the long‐range 1 H, 13 C correlations in the HMBC spectrum ( Figure ) and comparison to our previous data confirmed a 7‐ O ‐ β ‐ d ‐apiose‐(1→6)‐ β ‐ d ‐glucose as the sugar unit of compounds 2a / 2b .…”

“…guianensis have been described as well as the anti‐DENV activity of extracts from other Rubiaceae species when screened in hepatocarcinoma cell lineage (HepG2) revealing the significant antiviral effect of the MeOH extract of Faramea bahiensis leaves. The chemical study of this extract led to the isolation, among other, of the new antiviral flavanone apiofuranoside ( 1 ) . In this article, we report the in vitro non‐cytotoxic and anti‐dengue virus serotype 2 effects in HepG2 of the species Faramea hyacinthina and F .…”