Comprehensive characterisation of polyphenols in leaves and stems of three anti‐dengue virus type‐2 active Brazilian <i>Faramea</i> species (Rubiaceae) by HPLC‐DAD‐ESI‐MS/MS

Wolff, Thiago; Berrueta, Luis Á.; Valente, Lígia M. M.; Barboza, Rodolfo S.; Neris, Rômulo L. S.; Guimarães‐Andrade, Iris P.; Assunção‐Miranda, Iranaia; Nascimento, Adriana C. do; Gomes, Mario; Gallo, Blanca; Iriondo, C.

doi:10.1002/pca.2790

Cited by 10 publications

(6 citation statements)

References 42 publications

(117 reference statements)

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“…The fragmentation of [M þ H] þ ion of vicenin-2 seems to be complicated, however, it has been already described in details by Abad-Garc ıa et al 40 The relative abundances of the product ions shown in Figure 1 match very well the relative abundances reported by Wolff et al and are in acceptable agreement with the other data concerning the fragmentation of [M þ H] þ ion of vicenin-2. 27,28,40,41 The second flavone C-glycoside, detected in both Prunus avium and Prunus cerasus, was identified as apigenin 6-C-glucoside (isovitexin). Its exemplary product ion spectra are shown in Figure 2 (the other product ion spectra are shown in supplementary material, Figure 10s, 11s).…”

Section: Resultsmentioning

confidence: 99%

Detection of flavone C-glycosides in the extracts from the bark ofPrunus aviumL. andPrunus cerasusL.

Beszterda

Frański

2020

Eur J Mass Spectrom (Chichester)

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The extracts from the bark of Prunus avium and Prunus cerasus have been analyzed by using high pressure liquid chromatography/electrospray ionization mass spectrometry. For the first time in the bark of Prunus species flavonoid C-glycosides have been detected. On the basis of the characteristic fragmentation patterns of their [M-H]− and [M + H]+ ions, three flavonoid C-glycosides have been identified, namely apigenin-6,8-di-C-glucoside (vicenin-2), apigenin-6-C-glucoside (isovitexin) and chrysin-8-C-glucoside. Taking into account the widely studied biological activities of flavonoid C-glycosides, the barks of these common fruit trees seem to be interesting materials of potential medical or cosmetic application.

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Section: Resultsmentioning

confidence: 99%

Detection of flavone C-glycosides in the extracts from the bark ofPrunus aviumL. andPrunus cerasusL.

Beszterda

Frański

2020

Eur J Mass Spectrom (Chichester)

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“…As previously proposed, 58 The polyphenolic and glycoalkaloid profiles of the different parts of S. nigrum L. were characterised using UHPLC-QE-HRMS. As shown in Table 2, the distribution of polyphenolics and glycoalkaloids varied in different parts.…”

Section: The Fragmentation Pathways Of Flavonoidsmentioning

confidence: 99%

Bioactivities and chemical profiling comparison and metabolomic variations of polyphenolics and steroidal glycoalkaloids in different parts of Solanum nigrum L.

Zhang,

Liu,

Cui

et al. 2023

Phytochemical Analysis

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IntroductionSolanum nigrum L. is a traditional medicinal herb and edible plant. Many studies provide evidence that S. nigrum L. is a nutritious vegetable. Polyphenols and steroidal glycoalkaloids are the main components.ObjectivesThis study aimed to systemically evaluate the phytochemical profile, quantification, and bioactivities of polyphenolics and glycoalkaloids in different parts of S. nigrum L.ResultsTotal polyphenols (TPC) and total glycoalkaloids (TGK) were determined using the Folin–Ciocalteu and acid dye colorimetric methods, respectively. A total of 55 polyphenolic constituents (including 22 phenolic acids and 33 flavonoids) and 24 steroidal glycoalkaloids were identified from different parts using ultrahigh‐performance liquid chromatography Q‐exactive high‐resolution mass spectrometry (UHPLC‐QE‐HRMS), of which 40 polyphenols (including 15 phenolic acids and 25 flavonoids) and one steroidal glycoalkaloid were characterised for the first time in S. nigrum L. Moreover, typical polyphenols and glycoalkaloids were determined using HPLC‐UV and HPLC–evaporative light‐scattering detector (ELSD), respectively. In addition, the TPC and TGK and their typical constituents were compared in different anatomical parts. Finally, the antioxidant capacities of polyphenolic extracts from different parts of S. nigrum L. were evaluated by ·OH, 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging and ferric‐reducing antioxidant power (FRAP) assay in vitro. In addition, the antitumour effects of TGK from different parts of S. nigrum L. on the proliferation of PC‐3 cells were investigated using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay. Polyphenolic and glycoalkaloid extracts from different parts of S. nigrum L. showed different antioxidant and cytotoxic capacities in vitro.ConclusionThis is the first study to systematically differentiate between polyphenolic and glycoalkaloid profiles from different parts of S. nigrum L.

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“…The optimum cone voltages were those which produced the maximum intensity for protonated molecule [M+H] + and protonated aglycone ion [Y0] + in the previous MS experiments. Phenolic standards were supplied as previously described (Wolff et al 2019). All stock standard solutions (in concentrations ranging from 300 to 2700 μg mL -1 , depending on each phenolic compound, were prepared in MeOH.…”

Section: Hplc-dad-ms-ms Analysismentioning

confidence: 99%

Characterization of polyphenols in the methanol leaf extract of Bathysa gymnocarpa (Rubiaceae) by HPLC-DAD-ESI-MS/MS

Araujo

Cruz

Gomes

et al. 2023

Natural Product Research

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The species Bathysa gymnocarpa K.Schum is a tree belonging to the Rubiaceae family, endemic in Brazil. So far, there are reports neither of phytochemical work nor of biological evaluation of it. The analysis by High Performance Liquid Chromatography coupled to a Diode Array Detector and a tandem Mass Spectrometer with an Electrospray Ionization source (HPLC-DAD-ESI-MS/MS) of its crude extract allowed to characterize in a complex mixture, without isolation, fourteen compounds, being two as cinnamic acid derivatives, and the others as mono-, di-and triglycosilated derivatives of the flavonols quercetin and kaempferol. These compounds are reported for the first time in Bathysa spp.

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Comprehensive characterisation of polyphenols in leaves and stems of three anti‐dengue virus type‐2 active Brazilian Faramea species (Rubiaceae) by HPLC‐DAD‐ESI‐MS/MS

Abstract: This research allowed a detailed phenolic composition of three bioactive Faramea species to be achieved, thus contributing to the study of this genus and providing valuable information for further phytotherapeutic applications.

Cited by 10 publications

References 42 publications

Detection of flavone C-glycosides in the extracts from the bark ofPrunus aviumL. andPrunus cerasusL.

Detection of flavone C-glycosides in the extracts from the bark ofPrunus aviumL. andPrunus cerasusL.

Bioactivities and chemical profiling comparison and metabolomic variations of polyphenolics and steroidal glycoalkaloids in different parts of Solanum nigrum L.

Characterization of polyphenols in the methanol leaf extract of Bathysa gymnocarpa (Rubiaceae) by HPLC-DAD-ESI-MS/MS

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