Antiviral Activity of an Aqueous Extract of the CyanobacteriumMicrocystis aeruginosa

Nowotny, Andrea; Mentel, Renate; Wegner, Ursula; Mundt, S.; Lindequist, U

doi:10.1002/(sici)1099-1573(199703)11:2<93::aid-ptr46>3.0.co;2-g

Cited by 19 publications

(17 citation statements)

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“…In former experiments a strong effect of the crude aqueous extract of the collected field strain of Microcystis aeruginosa was observed [5]. The concentration of 50% inhibition of virus replication in MDCK cells was 11.0 lg/ml.…”

Section: Resultsmentioning

confidence: 98%

“…Methanolic and aqueous extracts of several genera, Microcystis, Nodularia, Oscillatoria, Scytonema, Lyngbya and Calothrix, were prepared as described previously [3,5]. Antiviral activity was evaluated using influenza virus A/WSN/33 (H1N1) London in Madin Darby canine kidney (MDCK) cells by colorimetric assay [7].…”

Section: Methodsmentioning

confidence: 99%

“…Protease inhibitory activity was monitored with BAPNA (a-Nbenzoyl-DL-arginine-p-nitroanilide) as a substrate for trypsin and colorimetric analysis at 405 nm. Fractionation and separation of active compounds was done by chromatographic and spectroscopic methods [5].…”

Section: Methodsmentioning

confidence: 99%

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Cyanobacteria a potential source of antiviral substances against influenza virus

Zainuddin

Mundt

Wegner

et al. 2002

Medical Microbiology and Immunology

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Aqueous and methanolic extracts of cultured cyanobacteria of several genera, Microcystis, Nodularia, Oscillatoria, Scytonema, Lyngbya and Calothrix were evaluated for their in vitro antiviral activity against influenza A virus in Madin Darby canine kidney cells. None of the methanolic extracts showed cytotoxic effects. The inhibitory concentration (IC 50 ) of antiviral activity ranged between 20.0 lg to 79.0 lg extract/ml. The most active extract in this screening derived from genus Microcystis. The further analysis of methanolic extracts of cultured strains of genus Microcystis revealed a remarkable antiviral activity against influenza A virus for M. aeruginosa, M. ichthyoblabe and M. wesenbergii.The observed antiviral activity was associated with protease inhibitory activity of approximately 90% and suggest that protease inhibitory activity may be responsible for reducing virus replication. These results show that cyanobacteria are able to produce compounds with biological activity that may be of potential clinical interest.

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Section: Resultsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

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Cyanobacteria a potential source of antiviral substances against influenza virus

Zainuddin

Mundt

Wegner

et al. 2002

Medical Microbiology and Immunology

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“…Antiviral assays. Antiviral activity was determined using the dye uptake assay (Nowotny et al, 1997;Rajbhandari et al, 2001). MDCK and Vero cells were seeded in 96-well tissue culture plates at an initial cell concentration of 60 000 cells/well and incubated for 24 h in a humidified 5% CO 2 atmosphere and preincubated with 100 µL of serial dilutions of plant extracts in medium (100, 50, 25, 12.5, 6.25, 3.1, 1.5 and 0.7 µg/mL) in triplicate for 30 min at 37°C.…”

Section: Plant Materials the Plants Were Collected By Dr Ramzimentioning

confidence: 99%

Phytochemical screening and antiviral activity of some medicinal plants from the island Soqotra

et al. 2006

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Methanol and hot-aqueous extracts of 25 different plant species, used in Yemeni traditional medicine and growing, partly as endemic plants, on the island Soqotra have been investigated for their antiviral activity. In addition, the phytochemical identification of the main chemical constituents was performed. The extracts were assayed in two in vitro viral systems, which used influenza virus type A/MDCK cells and herpes simplex virus type 1/Vero cells, at non-cytotoxic concentrations. The herpes simplex virus type 1 showed more sensitivity than the influenza virus type A against the extracts investigated. The methanol extracts of Boswellia ameero, Boswellia elongata, Buxus hildebrandtii, Cissus hamaderohensis, Cleome socotrana, Dracaena cinnabari, Exacum affine, Jatropha unicostata and Kalanchoe farinacea showed anti-influenza virus type A activity with 50% inhibition (IC50) concentrations ranging from 0.7 to 12.5 microg/mL. In addition, 17 plants of the 25 investigated exhibited anti-HSV-1 activity. The antiviral activity of some active extracts was also observed on a molecular level.

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“…It also showed an anti‐algal activity against the green alga Chlamydomonas neglecta (Ishida & Murakami ). The crude aqueous extract of M. aeruginosa showed antiviral activity against influenza A virus (Nowotny, Mentel, Wegner, Mundt & Lindequist ; Zainuddin, Mundt, Wegner & Mentel ). The present study was aimed at determining the immunostimulating (or immunosupressing) effect of Microcystis in Indian major carp, Labeo rohita , which is an important species in freshwater aquaculture, using application methodology relevant to practical farm conditions.…”

Section: Introductionmentioning

confidence: 99%

Microcystis aeruginosa(Kütz) incorporated diets increase immunity and survival of Indian major carpLabeo rohita(Ham.) againstAeromonas hydrophilainfection

et al. 2012

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The aim of this study was to evaluate dietary dosages of Microcystis on the immune response and disease resistance against infections due to the opportunistic pathogen Aeromonas hydrophila in Labeo rohita fingerlings. Labeo rohita fingerlings were fed diet containing 0 (Control), 0.5, 1.0 and 5 g Microcystis powder kg À1 dry diet for 90 days. Three replicate groups of fish averaging 20 ± 2 g were fed for 3 months daily. At 30 days interval, samples were assayed for different biochemical [serum total protein, albumin, globulin, albumin: globulin (A:G) ratio] and immunological (superoxide anion production, lysozyme and serum bactericidal activity) parameters. The results demonstrate that fish fed Microcystis showed increased levels of lysozyme, serum bactericidal activity, serum protein and albumin (P 0.05) as compared with the control group. After 90 days, fish were challenged with A. hydrophila and mortality (%) was recorded up to day 10 postchallenge. The group fed 1.0 g Microcystis kg À1 dry diet showed the highest percentage survival (72%). These results indicate that Microcystis aeruginosa stimulates the immunity and makes L. rohita more resistant to infection by A. hydrophila when fed in dried form in feed.

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Antiviral Activity of an Aqueous Extract of the CyanobacteriumMicrocystis aeruginosa

Cited by 19 publications

References 0 publications

Cyanobacteria a potential source of antiviral substances against influenza virus

Cyanobacteria a potential source of antiviral substances against influenza virus

Phytochemical screening and antiviral activity of some medicinal plants from the island Soqotra

Microcystis aeruginosa(Kütz) incorporated diets increase immunity and survival of Indian major carpLabeo rohita(Ham.) againstAeromonas hydrophilainfection

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