Cyanobacteria a potential source of antiviral substances against influenza virus

Abstract

Aqueous and methanolic extracts of cultured cyanobacteria of several genera, Microcystis, Nodularia, Oscillatoria, Scytonema, Lyngbya and Calothrix were evaluated for their in vitro antiviral activity against influenza A virus in Madin Darby canine kidney cells. None of the methanolic extracts showed cytotoxic effects. The inhibitory concentration (IC 50 ) of antiviral activity ranged between 20.0 lg to 79.0 lg extract/ml. The most active extract in this screening derived from genus Microcystis. The further analysis of methanolic extracts of cultured strains of genus Microcystis revealed a remarkable antiviral activity against influenza A virus for M. aeruginosa, M. ichthyoblabe and M. wesenbergii.The observed antiviral activity was associated with protease inhibitory activity of approximately 90% and suggest that protease inhibitory activity may be responsible for reducing virus replication. These results show that cyanobacteria are able to produce compounds with biological activity that may be of potential clinical interest.

Inhibitory effect of microalgae and cyanobacteria extracts on influenza virus replication and neuraminidase activity

“…Cyanobacteria are a promising yet underexplored source for novel natural products, including antiviral compounds. More detailed screening studies have identified antiviral activities on sulfoglycolipids and lectins ( Lopes et al, 2011 ; Mundt et al, 1997 ; Niedermeyer, 2015 ; Sharaf et al, 2010 ; Zainuddin et al, 2002 ). However, chemically different groups of compounds in cyanobacteria include alkaloids, lipopeptides, macrolides, and others ( Kiuru et al, 2014 ).…”

“…Aqueous and methanolic extracts of cultured cyanobacteria of Microcystis and Spirulina genus inhibit influenza replication with SI comparable to ethyl acetate extract from Leptolyngbya sp. (cyanobacteria) ( Chen et al, 2016 ; Zainuddin et al, 2002 ). Besides that, one sulfated polysaccharide purified from the marine microalga, Gyrodinium impudium , inhibits influenza A infection with SI higher than 200, but not influenza B ( Kim et al, 2012 ).…”

Evaluation of Microalgae Antiviral Activity and Their Bioactive Compounds

“…These peptides are generally synthesized by non-ribosomal peptides in combination with fatty acids in algae, sponges, and other marine organisms [ 106 ]. Zainuddin et al [ 107 , 108 ] showed that ichthyopeptin A, a CDP produced by Microcystis ichthyoblabe , had a high IAV activity inhibiting the proteins of the virus cycle.…”

Cyanobacteria and cyanometabolites used in the pharmaceutical and medical industry

“…Polysaccharides produced by cyanobacteria including A. platensis, demonstrated the ability to inhibit the replication of HSV, HIV-1, HIV-3, in uenza A, and mumps virus, which were cultured in suspensions of human cell cultures (Głowacka et al, 2007). Zainuddin et al (2002) researched the aqueous and methanolic extract from cyanobacterial cultures of the genus Calothrix, Microcystis, Nodularia, Oscillatoria, Lyngbya and Scytonema to check their activity against in uenza A virus in a dog's kidney cells. e most e ective extract turned out to be obtained from cyanobacteria of the genus Microcystis.…”