Antiurolithiatic and antioxidant activity of Hordeum vulgare seeds on ethylene glycol-induced urolithiasis in rats

Abstract

Objective:The objective was to investigate the antiurolithiatic and antioxidant activity of ethanolic extract of Hordeum vulgare seeds (EHV) on ethylene glycol-induced urolithiasis in Wistar albino rats.Materials and Methods:Urolithiasis was produced in Wistar albino rats by adding 0.75% v/v ethylene glycol (EG) to drinking water for 28 days. The ethanolic extract of Hordeum vulgare seeds (EHV) was assessed for its curative and preventive action in urolithiasis. In preventive treatment, the EHV given from 1st day to 28th day, while in the curative regimen, the EHV was given from 15th day to 28th day. Various renal functional and injury markers such as urine volume, calcium, phosphate, uric acid, magnesium, urea, and oxalate were evaluated using urine, serum, and kidney homogenate. Antioxidant parameters such as lipid peroxidation, superoxide dismutase, and catalase were also determined.Results:The EHV treatment (both preventive and curative) increased the urine output significantly compared to the control. The EHV treatment significantly reduced the urinary excretion of the calcium, phosphate, uric acid, magnesium, urea, and oxalate and increased the excretion of citrate compared to EG control. The increased deposition of stone forming constituents in the kidneys of calculogenic rats were significantly lowered by curative and preventive treatment with EHV. It was also observed that the treatment with EHV produced significant decrease in lipid peroxidation, and increased levels of superoxide dismutase and catalase.Conclusion:These results suggest the usefulness of ethanolic extract of Hordeum vulgare seeds as an antiurolithiatic and antioxidant agent.

A Study on Antiurolithiatic Activity of <i>Melia </i><i>A</i><i>zadirachta</i> L. Aqueous Extract in Rats

“…The extract showed a preventive activity of 59% and a curative activity of 46% against magnesium ammonium phosphate type of stones. This indicates the antiurolithiatic activity of Melia azadirachta in preventing both the formation of stones and also in dissolving the preformed stones which are in conformity with previous studies of Shah et al [33].…”

“…The excretion of oxalate and calcium were increased in curative group animals which is consistent with the previous reports [33,34]. Increased urinary calcium is a factor favouring the nucleation and precipitation of calcium oxalate or apatite (calcium phosphate) from urine and subsequent crystal growth [35].…”

“…All this supports the effectiveness of the aqueous extract of Melia azadirachta against prevention of stone formation and its ability to dissolve preformed stones. Biochemical changes as reflected in the urine chemistry are very important to understand about the pathogenesis of stone formation, as well as design some treatment for dissolution or prevention of stones [33].…”

A Review on Globally Used Antiurolithiatic Monoherbal Formulations Belonging to Boraginaceae, Brassicaceae, Malvaceae and Poaceae Families

“…[58] Pharmacological activities: Anti-inflammatory, antioxidant, diuretic , demulcent [9] , litholytic. [59] Antiurolithiatic spectrum (reported): Whole plant against whewellite. [60] Hyparrhenia hirta (L.) Stapf.…”

Evaluation of Antiurolithiatic Potentials of Hydro-Alcoholic Extract of Cucumis sativus L.

“…The slides were examined under a light microscope to study the architecture of the kidney and calcium oxalate deposits. 10 …”