Antityrosinase mechanism of ellagic acid in vitro and its effect on mouse melanoma cells

Abstract

The activities of ellagic acid in inhibiting mushroom tyrosinase and cell proliferation were evaluated in this research. The results of enzyme kinetics indicated that ellagic acid could effectively inhibit tyrosinase activity. The value of the semi‐inhibitory rate (IC50) was 0.2 ± 0.05 mM. Ellagic acid inhibited tyrosinase activity in a reversible manner and was a mixed tyrosinase inhibitor. Furthermore, ellagic acid had a good inhibitory effect on the proliferation of mouse melanoma B16 cells and could induce apoptosis. The results acquired from fluorescence spectroscopy revealed that the interaction of ellagic acid with tyrosinase depended on hydrogen bond and electrostatic force. In addition, computational docking showed that ellagic acid interacted with amino acid residues of tyrosinase (Asn19 and Lys372) by hydrogen bond and produced electrostatic interaction with amino residue Lys18. Practical applications In the present research, the antityrosinase mechanism of ellagic acid and its effect on mouse melanoma cells were investigated. This study suggested that ellagic acid had a strong inhibitory activity against tyrosinase and cell proliferation,which laid an experimental foundation for the development of new drugs and whitening products. The combined multispectral methods used in this research can be applied to the screening of other antityrosinase inhibitors, further promoting the development and utilization of tyrosinase inhibitors.

Impeding Minocycline Induced Hyperpigmentation by Pomegranate Extracting the Epidermis of Guinea Pig

“…The number of melanocytes was significantly decreased in Group C,(Table -2,column-4 & 5), compare to control group A. This was due to inhibition of melanocyte proliferation by ellagicacidrichpomegranate 28 proving that ellagic acid possesses strong inhibitory effects on proliferation of melanocytes.…”

“…It was observed that no significant effects were exhibited on the mean thickness of epidermis, melanin pigmentation deposition and extension as well as number of melanocytes when compared to control group A. 26,27,28…”

Ellagic acid inhibits high glucose‑induced injury in rat mesangial cells via the PI3K/Akt/FOXO3a signaling pathway

“…EA is a type of polyphenol compound, widely used in Chinese herbal medicines, such as sanguisorba officinalis and pomegranate peel ( 27 , 28 ). Numerous studies have demonstrated that EA possesses multiple biological activities in vitro ( 14 , 29 , 30 ). The present study explored whether EA could prevent the HG-induced injury of MCs and its possible mechanism.…”

“…Cells were incubated in a constant temperature incubator with 5% CO 2 at 37˚C. When the cells grew to 80% confluency, they were treated as follows: i) Normal control group (NC), MCs were cultured in DMEM with 5.5 mM glucose; ii) HG group, MCs were cultured in DMEM with 30 To detect the cytotoxic effect of EA on MCs, different concentrations of EA (0, 1, 5, 10, 20, 40 or 80 µM ) or 20 µM LY294002 were incubated with MCs under low glucose conditions at 37˚C for 24 h.…”

The preventive effect of ellagic acid on brain damage in rats via regulating of Nrf‐2, NF‐kB and apoptotic pathway

“…Similarly when we look at our results, the Nrf‐2 protein expression level increased in the EA + CCl 4 group by EA administration in comparison to the CCl 4 group. Huang, Chai et al (2019) stated that treatment with EGCG in rats exposed to formaldehyde, the EGCG antagonized formaldehyde‐induced neurotoxicity in a dose‐dependent manner by activating the Nrf‐2 signaling pathway. Firdaus et al (2018) investigated the preventive effect of EA against neurotoxicity in arsenic treated rats.…”