Antitumor effect of 1, 8-cineole against colon cancer

Abstract

Several essential oils possess pharmacological effects. Among the various constituents of essential oils, 1, 8-cineole has been shown to possess pharmacological effects such as anti-bacterial and anti-inflammatory effects. The effect of 1, 8-cineole on human colorectal cancer cells, however, has not reported previously. In this study, we have investigated the anti-proliferative effect of 1, 8-cineole on human colon cancer cell lines HCT116 and RKO by WST-8 and BrdU assays. The cytotoxicity of 1, 8-cineole was investigated by LDH activity and TUNEL staining. The mechanism of apoptosis by 1, 8-cineole was determined by western blot analyses. In in vivo study, RKO cells were injected into the SCID mice and the effect of 1, 8-cineole was investigated. Specific induction of apoptosis, not necrosis, was observed in human colon cancer cell lines HCT116 and RKO by 1, 8-cineole. The treatment with 1, 8-cineole was associated with inactivation of survivin and Akt and activation of p38. These molecules induced cleaved PARP and caspase-3, finally causing apoptosis. In xenotransplanted SCID mice, the 1, 8-cineole group showed significantly inhibited tumor progression compared to the control group. These results indicated 1, 8-cineole suppressed human colorectal cancer proliferation by inducing apoptosis. Based on these studies 1, 8-cineole would be an effective strategy to treat colorectal cancer.

Comparative Study of Green Sub- and Supercritical Processes to Obtain Carnosic Acid and Carnosol-Enriched Rosemary Extracts with in Vitro Anti-Proliferative Activity on Colon Cancer Cells

“…The cytotoxic activity of this latter compound has been demonstrated to be within the low millimolar range against human colorectal HCT116 cells [66], which is well above (e.g., in 30 µg extract/mL, the concentration of 1,8-cineole ranged from 1.32 to 9.03 µM) its concentration in the rosemary extracts of our present study. A similar potency has been recently reported for camphor monoterpene, exerting a 50% reduction in viability at concentrations of 5.5 and 4.5 mM, respectively in HT-29 and HCT116 cells [67].…”

Two-step sequential supercritical fluid extracts from rosemary with enhanced anti-proliferative activity

“…These results suggest that the activity of other rosemary constituents, in addition to carnosic acid and carnosol, against colon cancer cells cannot be dismissed. Indeed, as mentioned previously, these extracts were enriched in monoterpenes such as 1,8-cineole, alpha-pinene, camphor and terpineol, which have reported antiproliferative activity on different cancer cell models (Murata et al, 2013 andBayala et al, 2014). For example, Murata et al (2013) recently showed that 1,8-cineole induces specific apoptosis in human colon cancer cell lines HCT116 and RKO.…”

“…The relative composition of the oxygenated terpene derivatives, which are associated with the quality of the essential oils present in the extracts, ranged from 70 to 89%. Additionally, some of these components have been previously described as possessing antiproliferative activity as well (Bayala et al, 2014;Murata et al, 2013). With the increase of extraction pressure in the first SFE step from 150 to 300 bar (Process 2), the relative percentage of terpene hydrocarbons was favored, increasing from 4.56% to 25.85%.…”

Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells

“…The eucalyptol (50 mM) was able to regulate activity of p38 and caspase-3, inducing apoptosis and suppressing proliferation in the colorectal humam cancer (Murata et al 2013). Camphor has been atributed to present cytotoxic activity in human squamous cell carcinoma (HSC-2) (Cherneva et al 2012).…”