Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells

Abstract

Salvia offi cinalis (Lamiaceae) has been used in south of Brazil as a diary homemade, in food condiment and tea-beverage used for the treatment of several disorders. The objective of this study was to characterize chemical compounds in the hydroalcoholic (ExtHS) and aqueous (ExtAS) extract from Salvia offi cinalis (L.) by gas chromatography-mass spectrometry (GC-MS) and by high-resolution electrospray ionization mass spectrometry (ESI-QTOF MS/MS), evaluate in vitro ability to scavenge the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH • ) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS •+ ), catalase (CAT-like) and superoxide dismutase (SOD-like) activity, moreover cytotoxic by MTT assay, alterations on cell morphology by giemsa and apoptotic-induced mechanism for annexin V/propidium iodide. Chemical identifi cation sage extracts revealed the presence of acids and phenolic compounds. In vitro antioxidant analysis for both extracts indicated promising activities. The cytotoxic assays using tumor (Hep-2, HeLa, A-549, HT-29 and A-375) and in non-tumor (HEK-293 and MRC-5), showed selectivity for tumor cell lines. Immunocytochemistry presenting a majority of tumor cells at late stages of the apoptotic process and necrosis. Given the results presented here, Brazilian Salvia offi cinalis (L.) used as condiment and tea, may protect the body against some disease, in particularly those where oxidative stress is involved, like neurodegenerative disorders, infl ammation and cancer.

RP-HPLC/MS/MS Analysis of the Phenolic Compounds, Antioxidant and Antimicrobial Activities of Salvia L. Species

“…The findings of this study are consistent with the literature. It has been found that Brazilian S. officinalis has a DPPH scavenging activity comparable to gallic acid [57]. In addition S. palaestina essential oil showed an increasing DPPH radical scavenging activity in a concentration dependent manner (from 0.122 to 1.35 mg/mL), which was a higher weight concentration than the ones used in this study [58].…”

“…The results showed that ethanol and water extracts of all samples showed moderate radical scavenging activity when compared to standard antioxidants (Figure 2C,F). All of the Salvia species were able to donate electrons to neutralize the DPPH radical, showing a catalase-like, superoxide dismutase (SOD)-like activity [57]. The highest and lowest activities were observed in S. brachyantha and S. aethiopis , respectively.…”

Quantitative Analysis of Polyphenols and Biological Activity of Sage Macerates

“…In traditional medicine, it has been used for the treatment of different kinds of disorders including dyspepsia (such as heartburn and bloating), seizure, ulcers, gout, rheumatism, inflammation, dizziness, tremor, diarrhea, hyperglycemia, excessive sweating, atherosclerosis and inflammations in the throat and skin [2][3][4].…”

Evaluation of bioactivities and phenolic composition of extracts of Salvia officinalis L. (Lamiaceae) collected in Montenegro

“…Caffeic and rosmarinic acids have been frequently reported for S. officinalis extracts (Lu & Foo 1999, 2000, 2001Hossain et al 2010;Roby et al 2013;Martins et al 2015;Garcia et al 2016). They are considered to be potent antioxidant molecules (Khan et al 2016;Nicolai et al 2016;Adomako-Bonsu et al 2017) that also exhibit antimutagenic, anticarcinogenic, antiinflammatory and antiallergenic activities (Konishi et al 2005).…”