Antitumor and immunomodulatory activities of total flavonoids extract from persimmon leaves in H22 liver tumor-bearing mice

Chen, Li; Wei, Yundong; Zhao, Shichao; Zhang, Mengliang; Yan, Xiaoting; Gao, Xichao; Li, Jinxia; Gao, Yanbin; Zhang, Anwen; Gao, Ying

doi:10.1038/s41598-018-28440-8

Cited by 29 publications

(29 citation statements)

References 29 publications

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“…Mice received treatment 6 days after tumor implantation when the tumor diameter reached 5-8 mm. Mice were intravenously injected with 20 mg/kg/day TF (dissolved in 40% ethanol) or vehicle (ethanol) as a control (19). The treatment was continued for 10 days (20).…”

Section: Reverse-transcription Quantitative Pcr (Rt-qpcr)mentioning

confidence: 99%

Total flavonoids suppress lung cancer growth via the COX‑2‑mediated Wnt/β‑catenin signaling pathway

Han

Fang

et al. 2020

Oncol Lett

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The aim of the present study was to explore the anti-cancer effects of total flavonoids (TF) on lung cancer and to investigate the underlying mechanism. The inhibitory effect of TF on the proliferation of A549 cells in vitro was measured using an MTT assay. The apoptotic rate of TF-treated A549 cells was analyzed using flow cytometry and terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling. Migration and invasion assays were performed to investigate the anti-migration effect of TF on A549 cells. Reverse-transcription quantitative PCR was used to analyze BCL2-like 2, BCL2, Bax, Bad, cyclooxygenase 2 (COX-2), Wnt and β-catenin mRNA expression levels in A549 cells. The in vivo anti-cancer effect of TF was investigated in a subcutaneous xenograft model of lung cancer in BALB/c nude mice. The results obtained in the present study revealed that TF exerted a significant inhibitory effect on the proliferation of A549 cells in a dose-dependent manner (P<0.01). TF induced apoptosis of A549 cells, which exhibited increased and decreased expression of pro-and anti-apoptotic genes, respectively. Furthermore, TF had a significant inhibitory effect on the migration and invasion of A549 cells (P<0.01). The mRNA expression levels of COX-2, Wnt and β-catenin were significantly downregulated in TF-treated A549 cells compared with controls. Additionally, treatment with TF inhibited tumor growth in mice, with a tumor inhibition rate of 64.07% compared with the controls. TF exhibited significant tumor inhibitory effects in vivo by promoting the apoptosis of tumor cells. In conclusion, the results suggested that TF may regulate lung cancer growth via the COX-2-Wnt/β-catenin signaling pathway. TF may serve as a novel anti-cancer agent for the treatment of lung cancer.

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Section: Reverse-transcription Quantitative Pcr (Rt-qpcr)mentioning

confidence: 99%

Total flavonoids suppress lung cancer growth via the COX‑2‑mediated Wnt/β‑catenin signaling pathway

Han

Fang

et al. 2020

Oncol Lett

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“…Persimmon leaves have been used in folk medicine and consumed in health-promoting beverages, particularly as a commercial tea in Asia [11]. Recently, the leaves have become increasingly popular as a natural food additive in the food, pharmaceutical, and cosmetic industries due to their functional properties, including their anti-oxidant, anti-diabetic, anti-tumor, and immunological effects [11,12,13,14]. These potential health benefits are attributed to bioactive compounds in the persimmon leaves.…”

Section: Introductionmentioning

confidence: 99%

“…These potential health benefits are attributed to bioactive compounds in the persimmon leaves. Many studies have been focused on low-molecular-weight phytochemicals in persimmon leaves, such as tannins, flavonoids, triterpenoids, and vitamin C [10,12,14,15,16]. However, in recent years, researchers have begun to investigate polysaccharides with relatively higher molecular weights in persimmon leaves.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of Structural and Immunological Features of Polysaccharides from Persimmon Leaves at Different Maturity Stages

et al. 2019

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In this study, we investigated changes in the structural and immunological features of polysaccharides (S1-PLE0, S2-PLE0, and S3-PLE0) extracted from persimmon leaves at three different growth stages. Physicochemical analyses revealed that their chemical compositions, molecular weight distributions, and linkage types differed. High-performance size-exclusion chromatograms showed that the molecular weights of the polysaccharides increased during successive growth stages. In addition, seasonal variation of persimmon leaves affected the sugar compositions and glycosidic linkages in the polysaccharides. S2-PLE0 was composed of comparatively more galactose, arabinose, rhamnose, xylose, and galacturonic acid, showing the presence of β-glucopyranoside linkages. Significant differences also occurred in their immunostimulatory effects on RAW264.7 macrophages, with respect to which their activities could be ordered as S2-PLE0 > S3-PLE0 > S1-PLE0. Evidently, S2-PLE0 showed the greatest immunostimulatory activity by enhancing the phagocytic capacity and promoting nitric oxide (NO) and cytokines secretion through the upregulation of their gene expression in macrophages. These results suggest that differences in the structural features of polysaccharides according to the different maturity of persimmon leaves might impact their immunostimulatory properties. The results also provide a basis for optimizing persimmon leaf cultivation strategies for food and medical uses of the polysaccharides.

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“…Pereira et al [25] evaluated the antioxidant effects of C. multijuga bark ethanolic extract and observed a higher concentration of phenolic compounds, such as epicatechin and epiafzelechin-condensed tannins. Increased NK activity observed in this study may be a consequence of the condensed tannins present in C. multijuga extract since condensed tannins upregulate NK cell response [22,23].…”

Section: /8mentioning

confidence: 69%

“…Immunomodulatory effects are also attributed to phenolic compounds and flavonoids [22][23][24]. Pereira et al [25] evaluated the antioxidant effects of C. multijuga bark ethanolic extract and observed a higher concentration of phenolic compounds, such as epicatechin and epiafzelechin-condensed tannins.…”

Section: /8mentioning

confidence: 99%

Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract

Rodrigues

Albiero

Nery

et al. 2019

Sci Med

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AIMS: Copaifera multijuga Hayne oleoresin is commonly used in traditional medicine owing to its anti-inflammatory, antiseptic, antitumor, and antibacterial properties. However, little is known about the effect of the compounds from the bark of this plant. In this study, the immunomodulatory effect of the ethanolic extract of C. multijuga bark via natural killer activity of non-adherent spleen cells of Ehrlich tumor-bearing mice was evaluated.METHODS: Male Swiss mice were inoculated subcutaneously with 1×106 Ehrlich tumor cells (Ehrlich and Ehrlich/C. multijuga group) or phosphate buffered saline solution (control group and C. multijuga group) and treated orally daily with C. multijuga extract (200 mg kg-1, 0.1 mL per mouse, for the Ehrlich/C. multijuga and C. multijuga groups) or phosphate buffered saline solution (control group and Ehrlich group). The four experimental groups consisted in eight mice each and were organized in two sets, one treated for seven days and another treated for 14 days, totalizing 64 mice throughout the experiment. Twenty-four hours after the last oral administration, the mice were euthanized and the spleen tissue was isolated to prepare a non-adherent spleen cell suspension and to evaluate natural killer activity. Data are presented as the cell lysis percentage of Yac.1 target cells by non-adherent spleen cells.RESULTS: Treatment for seven days increased natural killer activity in the Ehrlich/C. multijuga group (21.20±8.89, p<0.05) compared to the control group (3.14±2.71, p<0.05); however, this effect was not maintained in the groups treated for 14 days (Control: 6.02±6.98, Ehrlich: 4.82±7.72, C. multijuga: 2.07±2.10, Ehrlich/C. multijuga: 2.01±1.63, p>0.05).CONCLUSIONS: Treatment for seven days with an ethanolic extract of C. multijuga bark enhanced the natural killer activity of non-adherent spleen cells from Ehrlich tumor-bearing mice.

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Antitumor and immunomodulatory activities of total flavonoids extract from persimmon leaves in H22 liver tumor-bearing mice

Cited by 29 publications

References 29 publications

Total flavonoids suppress lung cancer growth via the COX‑2‑mediated Wnt/β‑catenin signaling pathway

Total flavonoids suppress lung cancer growth via the COX‑2‑mediated Wnt/β‑catenin signaling pathway

Discrimination of Structural and Immunological Features of Polysaccharides from Persimmon Leaves at Different Maturity Stages

Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract

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