Antitumor and antimicrobial activity of fatty acids from green microalga Coelastrella sp. BGV

Toshkova-Yotova, Tanya; Georgieva, A. I.; Iliev, Ivan; Alexandrov, Svetoslav; Ivanova, Alexandra; Пиларски, Пламен; Toshkova, Reneta

doi:10.1016/j.sajb.2022.04.003

Cited by 6 publications

(3 citation statements)

References 49 publications

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“…They linked this antibacterial action to the presence of a combination of abundant bioactive constituents of unsaturated FAs and polyphenolics. Additionally, Yotova et al [33] studied the FAs profile in the green coccid Coelastrella sp. BGV and identified 11 different FAs, within which, monounsaturated oleic acid (C18:1, ω-9) was present in the largest amount at 31.7%, followed by polyunsaturated linoleic acid (C18:2, ω-6; 25.9%) and saturated palmitic acid (C16:0; 19.2%).…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Bioactive Compounds Extracted from the Egyptian Untapped Green Alga Rhizoclonium hieroglyphicum

Morsi

El-Sabbagh

Mehesen³

et al. 2023

Water

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Finding alternative powerful antibacterial drugs of natural origins is, today, a crucial prerequisite due to the resistance of some bacterial strains to commercial and widely-used medications. Algae are characterized by their bioactive constituents and have a wide spectrum of biotechnological aspects, particularly antibacterial implications. During this study, four concentrations (5, 10, 20, and 40 mg mL–1) of the Egyptian untapped green microalga Rhizoclonium hieroglyphicum (Chlorophyta) were prepared using the polar solvents ethanol, methanol, and acetone. The antibacterial activity of the above-mentioned extracts was assessed, using the agar disc diffusion technique against three pathogenic bacteria, Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, and Pseudomonas aeruginosa ATCC 9027, which was compared to standard antibiotics. The minimal inhibitory concentrations (MICs) were also assessed and determined using a broth dilution assay. Our findings revealed that the R. hieroglyphicum ethanolic extract exhibited the most potent antibacterial effect and its MICs values were 0.533, 2.25, and 5.34 mg mL–1 against P. aeruginosa, E. coli, and S. aureus, respectively. A gas chromatography–mass spectrometry (GC–MS) approach to the crude R. hieroglyphicum ethanolic extract uncovered 30 different bioactive constituents, mainly including long-chain polyunsaturated and saturated fatty acids such as myristic (C14:0), palmitic (C16:0), stearic (C18:0), α-linolenic (C18:3; ω–3), and oleic (C18:1, ω–9) acids, which synergistically make this potent antibacterial action. The mechanism of action of these fatty acids was also discussed. Conclusively, R. hieroglyphicum could be a good candidate for the production and development of promising antibacterial agents.

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Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Bioactive Compounds Extracted from the Egyptian Untapped Green Alga Rhizoclonium hieroglyphicum

Morsi

El-Sabbagh

Mehesen³

et al. 2023

Water

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“…Similarly, a strain of Coelastrella sp. (same genus as CM2) with a fatty acids profile similar to that of CM2 strain has proved potent against E. coli UPEC, P. aeruginosa and Klebsiella pneumoniae [ 52 ]. However, no substantive conclusion can be drawn based on this, as the fatty acid composition of the multi-species cultures was not analyzed at the screening step of this study.…”

Section: Discussionmentioning

confidence: 99%

Removal of Antibiotic Resistance Genes, Class 1 Integrase Gene and Escherichia coli Indicator Gene in a Microalgae-Based Wastewater Treatment System

et al. 2022

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Microalgae-based wastewater treatment systems (AWWTS) have recently shown promise in the mitigation of antibiotic resistance genes (ARGs) from municipal wastewater (MWW). However, due to the large number of ARGs that exist in MWW, the use of indirect conventional water quality parameters to monitor ARGs reduction in wastewater would make the process less burdensome and economically affordable. In order to establish a robust relationship between the ARGs and water quality parameters, the current study employed different microalgae strains in monoculture (CM2, KL10) and multi-species combinations (CK and WW) for the MWW treatment under outdoor environmental conditions. The studied genes were quantified in the MWW influents and effluents using real-time PCR. All the cultures substantially improved the physicochemical qualities of the MWW. Out of the 14 genes analyzed in this study, tetO, tetW, tetX and ermB were decreased beyond detection within the first 4 days of treatment in all the cultures. Other genes, including blaCTX, sul1, cmlA, aadA, int1 and uidA were also decreased beyond a 2 log reduction value (LRV). The mobile genetic element, int1, correlated positively with most of the ARGs, especially sul1 (r ≤ 0.99, p < 0.01) and aadA (r ≤ 0.97, p < 0.01). Similarly, the Escherichia coli indicator gene, uidA, correlated positively with the studied genes, especially with aadA, blaCTX, blaTEM and cmlA (r ≤ 0.99 for each, p < 0.01). Some of the studied genes also correlated positively with total dissolved solids (TDS) (r ≤ 0.98, p < 0.01), and/or negatively with total suspended solids (TSS) (r ≤ −0.98, p < 0.01) and pH (r ≤ −0.98, p < 0.01). Among the tested cultures, both monocultures, i.e., KL10 and CM2 were found to be more consistent in gene suppression than their multi-species counterparts. The findings revealed water quality parameters such as TDS, TSS and E. coli as reliable proxies for ARGs mitigation in AWWTS and further highlight the superiority of monocultures over multi-species cultures in terms of gene suppression from the MWW stream.

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“…The antibacterial activity expected positively correlates with the presence of fatty acid derivatives of the extract according to the result of GC-MS analysis. Toshkova-Yotova et al (2022) mentioned that fatty acid was associated with antibacterial activity which are responsible to inhibit the growth of gram-positive and gram-negative bacteria. Besides, the presence of terpenoids and phenolic compounds are known as antibacterial activity (Daud et al 2021).…”

Section: Antibacterial Activitymentioning

confidence: 99%

The influence of fermentation using marine yeast Hortaea werneckii SUCCY001 on antibacterial and antioxidant activity of Gracilaria verrucosa

et al. 2022

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Abstract. Fatmawati F, Sibero T, Trianto A, Wiyanti DP, Sabdono A, Pronggenies D, Radjasa OK. 2022. The influence of fermentation using marine yeast Hortaea werneckii SUCCY001 on antibacterial and antioxidant activity of Gracilaria verrucosa. Biodiversitas 23: 5258-5266. Gracilaria verrrucosa is producer of agar, a polysaccharide, that widely used in pharmaceutical and food industries. However, several studies have shown that the secondary metabolite of this seaweed is not potential as an antimicrobial and antioxidant agents. This study aimed to determine the antibacterial and antioxidant properties of methanolic extract of G. verrucosa after fermentation using marine yeast Hortaea werneckii SUCCY001. The samples were fermented based on different optimization times, specially in three, six and nine days. Samples were extracted using methanol by maceration method. Then the extracts were tested for antimicrobial potential against the several human clinical pathogens namely Bacillus cereus, Bacillus subtilis, Escherichia coli, Micrococcus luteus and Pseudomonas aeruginosa. The antioxidant potential was analyzed using the 1,1-diphenyl-2-picrylhydracyl (DPPH) and 2,2-Azinobis 3-ethyl benzothiazoline 6- sulfonic acid (ABTS) method while total phenolic content (TPC) was measured using Folin-Ciocalteu. Secondary metabolites were characterized using thin layer chromatography (TLC) and GC-MS. TLC plate visualization using UV light, DPPH, FeCl3 and vanillin-sulfuric acid reagents. The results showed that the extract fermented for nine days was able to inhibit the growth of P. aeruginosa and M. luteus at a concentration of 2 mg/mL. The highest antioxidant activity was found in the nine days fermented extract with an RSA value of 21.9% ± 0,0001 for DPPH and 12.53 % ± 0.002 for ABTS. Furthermore, the highest total phenolic content was found in the nine days fermented extract of 7.57 GAE/mg. The results of characterization using TLC showed the presence of phenolic compounds, terpenoids and antioxidants in fresh seaweed extracts, autoclaved fresh seaweed, three days of fermentation, six days and nine days. Based on this research, it can be seen that the addition of marine yeast starter H. werneckii SUCCY001 was able to increase the antibacterial and antioxidant activity of G. verrucosa.

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Antitumor and antimicrobial activity of fatty acids from green microalga Coelastrella sp. BGV

Cited by 6 publications

References 49 publications

Antibacterial Activity of Bioactive Compounds Extracted from the Egyptian Untapped Green Alga Rhizoclonium hieroglyphicum

Antibacterial Activity of Bioactive Compounds Extracted from the Egyptian Untapped Green Alga Rhizoclonium hieroglyphicum

Removal of Antibiotic Resistance Genes, Class 1 Integrase Gene and Escherichia coli Indicator Gene in a Microalgae-Based Wastewater Treatment System

The influence of fermentation using marine yeast Hortaea werneckii SUCCY001 on antibacterial and antioxidant activity of Gracilaria verrucosa

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