Antitumor Activity of Triterpenes in Inonotus obliquus

Kahlos, K.; Kangas, Lauri; Hiltunen, R.

doi:10.1055/s-2007-969367

Cited by 31 publications

(24 citation statements)

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“…This fungus grows on birch trees in cold northern climates. Since the sixteenth century, this fungus has been used as a folk medicine due to its antitumor properties against several types of cancer cells (Kahlos et al, 1986;Saar, 1991). In some cases, the therapeutic efficiency of I. obliquus has been demonstrated clinically.…”

Section: Introductionmentioning

confidence: 99%

“…For example, a decoction of fungal sclerotia did not show toxic effects and has been used in the treatment of cancers and digestive system diseases Nakata et al, 2007). Recently, in addition to polysaccharides, many polyphenolic compounds such as triterpenoids, steroids and ergosterol peroxides from I. obliquus have been found to possess biological activities, including antioxidant, antibacterial, hepatoprotective, platelet aggregation inhibitory and antitumor effects (Kahlos et al, 1986;Shin et al, 2001;Park et al, 2004;Cui et al, 2005;Hyun et al, 2006;Mazurkiewicz, 2006;Lee et al, 2007;Nakajima et al, 2007;Shi et al, 2008;Youn et al, 2008). However, the molecular mechanisms by which these antitumor effects occur are not well understood.…”

Section: Introductionmentioning

confidence: 99%

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Antitumor activity of water extract of a mushroom, Inonotus obliquus, against HT‐29 human colon cancer cells

Lee

Hwang

Yun

2009

Phytotherapy Research

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In the current study, it was demonstrated that the hot water extract of I. obliquus (IOWE) exerts inhibitory activity against the proliferation of human colon cancer cells (HT-29). The inhibitory effect of IOWE on the growth of HT-29 cancer cells was evaluated by treating cells with IOWE at concentrations of 0.25, 0.5 and 1.0 mg/mL for 24 or 48 h. The IOWE inhibited cell growth in a dose-dependent manner, and this inhibition was accompanied by apoptotic cell death. The maximum inhibitory effect (56%) was observed when IOWE was treated at a concentration of 1.0 mg/mL for 48 h. The apoptotic effect of IOWE on HT-29 cells was also confirmed by flow cytometric analysis. In addition, the apoptotic cell percentage was closely associated with down-regulation of Bcl-2 and up-regulation of Bax and caspase-3. The results suggest that IOWE would be useful as an antitumor agent via the induction of apoptosis and inhibition of the growth of cancer cells through up-regulation of the expression of proapoptotic proteins and down-regulation of antiapoptotic proteins.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antitumor activity of water extract of a mushroom, Inonotus obliquus, against HT‐29 human colon cancer cells

Lee

Hwang

Yun

2009

Phytotherapy Research

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“…1,9) Antitumor effects of several extracts and isolated compounds from fungus species has been demonstrated in vitro and in animal models where several triterpenes and ergosterol peroxide is believed to contribute to its activity. 10,11) In the present work, it was evaluated the antitumor activity of pisosterol using sarcoma 180 bearing mice. Sarcoma 180 is a mouse-originated tumor and one of the most frequently used cell lines in antitumor related research in vivo.…”

Section: Discussionmentioning

confidence: 99%

Antitumor Activity of Pisosterol in Mice Bearing with S180 Tumor

Montenegro

Farias

Pereira

et al. 2008

Biological & Pharmaceutical Bulletin

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Triterpenes are products of secondary metabolism of different organisms, being very common in fungus species. One single fungus' specie can produce a high number of active compounds, with different pharmacological effects.1) Gamoderma lucidum, as an example, produces over 120 different triterpenes, and also other bioactive compounds.2) Several species has been studied for their biological activities, such as antiviral, anticomplement, antiatherogenic, antitumour and hepatoprotective activities. 1)Pisosterol (Fig. 1), a triterpene isolated from the ectomicorhizal Pisolithus tinctorius, has been reported to strongly inhibit tumor cells proliferation in vitro, while it had no antimitotic effect on sea urchin eggs.3) Classical cytotoxic drugs tested in the sea urchin eggs and cancer cells have shown to be active in both assays, although compounds may present a higher IC 50 value in the sea urchin eggs than in tumor cells, as observed for doxorubicin and etoposide. 4,5) The sea urchin eggs assay can detect cytotoxic agents such as DNA synthesis inhibitors, RNA synthesis inhibitors, microtubule assembly inhibitors, and protein synthesis inhibitors. 6)Thus, the absence of activity of pisosterol in this assay suggested a different mechanism of action. In fact, Montenegro et al. (2007) 7) demonstrated that the inhibition of leukemia (HL-60) cells proliferation is related to monocytic cell-like differentiation induction, followed by apoptosis. These findings may explain the selectivity of pisosterol to cancer cells, and reinforce the anticancer potential of this naturally occurring substance.In the present study, the in vivo antitumor activity of pisosterol was evaluated using sarcoma 180 transplanted in mice. Histopathological and morphological analyses of the tumor and organs, including liver, spleen and kidney were performed in order to evaluate the toxicological aspects of the pisosterol treatment. MATERIALS AND METHODSPisosterol Isolation Pisosterol, in its pure form, was isolated from the ectomicorhizal fungus Pisolithus tinctorius collected in a Eucalyptus plantation at Brejo, Maranhão, Northeast Brazil as described previously.3)The structure of pisosterol ( Melo, 1127, P.O. Box 3157, 60430-270 Fortaleza, Ceará, Brasil: b Departamento de Clínica Odontológica, Universidade Federal do Ceará; and c Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará; Fortaleza, Ceará, Brasil. Received August 31, 2007; accepted December 5, 2007 It has been reported that pisosterol was active against cancer cells but lacked activity on the development of sea urchin eggs. Recently, it was reported that pisosterol induces differentiation in leukemia cell line. The present study evaluated the in vivo antitumor activity of pisosterol a triterpene isolated from Pisolithus tinctorius. Histopathological and morphological analyses of the tumor and the organs, including liver, spleen and kidney, were performed in order to evaluate toxicological aspects associated with pisosterol treatments. Antitumor activity of...

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“…Клеточные линии Ингибирующая концентрация А аденокарциномы молочной железы MCF-7 1 мкг/мл; 250 мкг/мл [7,9,24] лейкемии Р388 более 100 мкг/мл [23] аденокарциномы шейки матки HeLa 250 мкг/мл [31] карциномы лёгких А-549 250 мкг/мл [23] аденокарциномы желудка AGS 250 мкг/мл [31] карциномы молочной железы MDA-MB-231 более 40 мкг/мл [35] карциномы простаты РС3 более 40 мкг/мл [35] лейкемии L1210 более 80 мкг/мл [23] аденокарциномы желудка COLO205 более 40 мкг/мл [23,25] А1 аденокарциномы молочной железы MCF-7 1 мкг/мл; 250 мкг/мл [7,9,24] карциносаркомы Уолкера 256 10 мкг/мл [9] лейкемии Р388 6 мкг/мл; более 40 мкг/мл [20,24] лейкемии L1210 49 мкг/мл [23] аденокарциномы желудка COLO205 75 мкг/мл [23] карциномы молочной железы MDA-MB-231 более 40 мкг/мл [35] карциномы простаты РС3 более 40 мкг/мл [35] аденокарциномы шейки матки HeLa 250 мкг/мл [31] карциномы лёгких А-549 250 мкг/мл [23,24] аденокарциномы желудка AGS 250 мкг/мл [31] А2 аденокарциномы молочной железы MCF-7 5 и 10 мкг/мл [7,9] лейкемии Р388 12 мг/мл [20] лейкемии L1210 16 мкг/мл [23] карциномы молочной железы MDA-MB-231 25 мкг/мл [35] карциномы простаты РС3 29 мкг/мл [35] аденокарциномы желудка COLO205 более 90 мкг/мл [23,24] карциномы лёгких А-549 более 90 мкг/мл [23,24] А5 аденокарциномы молочной железы MCF-7 250 мкг/мл [7,9] аденокарциномы шейки матки ...…”

Section: веществоunclassified

Composition and biological activity of triterpenes and steroids from Inonotus obliquus (chaga)

2016

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Систематизированы данные по химическому составу веществ тритерпеновой и стероидной природы, выделенных из чаги, как выращенной в условиях искусственной культуры, так и произрастающей в природе. Приведены сведения по биологической активности экстрактов из чаги и этих соединений в отношении различных линий клеток рака in vitro и in vivо. Проведённый анализ позволил выявить определенные закономерности в эффективности подавления роста различных линий клеток. Установлено, что наибольшей активностью обладают тритерпеновые соединения, содержащие ОН-группу при С-22 и ненасыщенную связь в боковой цепи.Ключевые слова: чага, тритерпеновые соединения, стероидные соединения, биологическая активность, in vivo, in vitro DOI 10.18097/PBMC20166204369Рисунок. Общая структурная формула тритерпенов ланостанового ряда (а) и стероидных соединений (б).

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Antitumor Activity of Triterpenes in Inonotus obliquus

Cited by 31 publications

References 0 publications

Antitumor activity of water extract of a mushroom, Inonotus obliquus, against HT‐29 human colon cancer cells

Antitumor activity of water extract of a mushroom, Inonotus obliquus, against HT‐29 human colon cancer cells

Antitumor Activity of Pisosterol in Mice Bearing with S180 Tumor

Composition and biological activity of triterpenes and steroids from Inonotus obliquus (chaga)

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