Antitumor Activity of the Methanol Extract of Hypericum hookerianum Stem Against Ehrlich Ascites Carcinoma in Swiss Albino Mice

Dongre, Santoshkumar H.; Badami, Shrishailappa; Natesan, Senthilkumar; Honrao, Chandrashekhar

doi:10.1254/jphs.fp0061088

Cited by 27 publications

(11 citation statements)

References 21 publications

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“…Previous studies have noted the bioactivies of the Hypericum genus including the antitumor, antioxidant, antimicrobial and natural killer (NK) cell-activating activity (8)(9)(10)(11)(12). HJT, an annual herb from the genus Hypericum L., known in traditional Chinese medicine as Tian-ji-huang, grows mainly throughout southern China.…”

Section: Discussionmentioning

confidence: 99%

The effect-enhancing and toxicity-reducing activity of Hypericum japonicum Thunb. extract in murine liver cancer chemotherapy

Lü

Cao

Zhen-hua

et al. 2012

Molecular and Clinical Oncology

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Abstract. Chinese herbs are potential sources of antitumor drugs with immunoregulatory activity and few adverse effects. In the present study, we investigated whether the Hypericum japonicum Thunb. (HJT) extract enhanced the efficacy of 5-fluorouracil (5-FU) treatment in murine liver tumor xenografts and reduced toxicity of chemotherapy in hepatoma H22-bearing mice. Tumor weight and inhibition rate, thymus and spleen indices, as well as white blood cell (WBC) count were calculated. The phagocytic function of macrophages was assessed by observing peritoneal macrophages phagocytized chicken red blood cells (RBC). Body weight and toxic reactions of the chemotherapeutic and life prolongation rate were investigated in the mice. Results demonstrated that the HJT extract significantly enhanced the tumor inhibition rate of 5-FU, improved the immune function, reduced the toxic effects and prolonged the survival time in the tumor-bearing mice. Taken together, these results indicated that the HJT extract has a synergistic tumor-inhibiting effect with 5-FU, is able to reduce the toxic side effects and is likely to be safe and efficacious for use in antitumor therapy. IntrodutionLiver cancer is a malignant disease with a high incidence and mortality, and is often diagnosed at an advanced stage (1). Common therapies, such as surgical resection, percutaneous or transarterial interventions are of limited efficacy (2), as chemotherapy, one of the main measures for a comprehensive treatment, may induce adverse effects such as bone marrow suppression, gastrointestinal reactions and lowered immunological function.Several Chinese herbs have been identified as potential sources of antitumor drugs (3-5). The effect-enhancing and toxicity-reducing activity of Chinese herbs in radiotherapy and chemotherapy of tumors was previously confirmed (6,7). However, no studies focusing on the antitumor and toxicity-reducing effects of Hypericum japonicum Thunb. (HJT) have been reported thus far. Therefore, the present investigation was conducted to ascertain whether the HJT extract enhanced the efficacy of 5-fluorouracil (5-FU) treatment in murine liver tumor xenografts, and reduced toxicity of chemotherapy in tumor-bearing mice. Materials and methodsReagents. RPMI-1640 medium, fetal bovine serum (FBS), penicillin G and streptomycin were obtained from Invitrogen Corp. (Carlsbad, CA, USA). 5-FU was obtained from the Shanghai Xudong Haipu Pharmaceutical Co., Ltd. (Shanghai, China). Murine H22 hepatoma cells were purchased from the Shanghai Institute of Cell Biology at the Chinese Academy of Sciences (Shanghai, China). Animals. Institute of Cancer Research (ICR) mice(male/female, 50-50%; weight, 20±2 g) were purchased from the Experimental Animal Center, Zhengzhou University (Zhengzhou, China). The animals were kept in a standard laboratory, under a 12-h light/dark cycle, at room temperature (20±2˚C), in a humid (75±15%) and filtered laminar air flow-controlled room in the animal facility of the Experimental Animal Center of the Zhengzhou University...

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Section: Discussionmentioning

confidence: 99%

The effect-enhancing and toxicity-reducing activity of Hypericum japonicum Thunb. extract in murine liver cancer chemotherapy

Lü

Cao

Zhen-hua

et al. 2012

Molecular and Clinical Oncology

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“…Hypericum hookerianum is well known in folklore medicine for its different therapeutic potentials, including antidepressant, spasmolytic, stimulant, hypotensive and antifungal activities (Asolkar, Kakkar, & Chakre, 1992). In earlier studies, we reported the antitumour (Dongre, Badami, Natesan, & Raghu, 2007), cytotoxic (Vijayan et al, 2003) and CNS activities (Mukherjee, Suresh, & Verpoorte, 2001) of H. hookerianum. So far, antioxidant studies of this plant have not been reported.…”

Section: Introductionmentioning

confidence: 85%

Antioxidant activity ofHypericum hookerianumWight and Arn

Honrao¹,

Venkatesh²,

Ponnusankar³

et al. 2009

Natural Product Research

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Methanolic extracts of leaf, root, flower and aerial parts of Hypericum hookerianum were assessed for in vitro antioxidant activity using eight different models. Total antioxidant capacity, phenol and flavanol content of the extracts were determined to correlate between their antioxidant activity and constituents present therein. Results of in vitro antioxidant study suggest that extracts from leaf and flower have strong antioxidant potential. Leaf extract (100 & 200 mg kg(-1) b.w. p.o.) that showed maximum activity was selected for in vivo antioxidant studies using a CCl(4)-intoxicated rat model. The effects of extracts on lipid peroxidation (LPO), superoxide dismutase (SOD) and catalase (CAT) in serum and liver homogenate were analysed. CCl(4) treatment caused a significant increase in the level of CAT and SOD and a significant decrease in the level of LPO in a dose-dependent manner when compared to CCl(4) treated control. The results indicate the strong antioxidant nature of H. hookerianum leaf extract.

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“…A fraction of this extract displayed 60% cell growth inhibition at a concentration of 0.95 lg/ml (Skalkos et al 2005). Lately, Dongre et al (2007) reported antitumor activity of the methanol extract of Hypericum sp. against Ehrlich Ascites Carcinoma in swiss albino mice.…”

Section: Hypericum Perforatum Linnmentioning

confidence: 98%

Anticancer potential of Himalayan plants

et al. 2010

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Plants used in traditional medicine have stood up to the test of time and contributed many novel compounds for preventive and curative medicine to modern science. India is sitting on a gold mine of well recorded and traditionally well practiced knowledge of herbal medicine. Specially, plants growing at high altitude in Himalayan pastures are time-honored sources of health and general well being of local inhabitants. As of today, Himalayan plants are a major contributor to the herbal pharmaceutical industry both of India and other countries. Plants growing at higher altitudes are subjected to an assault of diverse testing situations including higher doses of mutagenic UV-radiation, physiological drought, desiccation and strong winds. Plants interact with stressful environments by physiological adaptation and altering the biochemical profile of plant tissues and producing a spectrum of secondary metabolites. Secondary metabolites are of special interest to scientists because of their unique pharmacophores and medicinal properties. Secondary metabolites like polyphenols, terpenes and alkaloids have been reported to possess antimutagenic and anticancer properties in many studies. The fundamental aspiration of the current review is to divulge the antimutagenic/anticancer potential of five alpine plants used as food or medicine by the populations living at high altitudes.

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Antitumor Activity of the Methanol Extract of Hypericum hookerianum Stem Against Ehrlich Ascites Carcinoma in Swiss Albino Mice

Cited by 27 publications

References 21 publications

The effect-enhancing and toxicity-reducing activity of Hypericum japonicum Thunb. extract in murine liver cancer chemotherapy

The effect-enhancing and toxicity-reducing activity of Hypericum japonicum Thunb. extract in murine liver cancer chemotherapy

Antioxidant activity ofHypericum hookerianumWight and Arn

Anticancer potential of Himalayan plants

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