Antitumor activity of colloidal silver on MCF-7 human breast cancer cells

Abstract

BackgroundColloidal silver has been used as an antimicrobial and disinfectant agent. However, there is scarce information on its antitumor potential. The aim of this study was to determine if colloidal silver had cytotoxic effects on MCF-7 breast cancer cells and its mechanism of cell death.MethodsMCF-7 breast cancer cells were treated with colloidal silver (ranged from 1.75 to 17.5 ng/mL) for 5 h at 37°C and 5% CO2 atmosphere. Cell Viability was evaluated by trypan blue exclusion method and the mechanism of cell death through detection of mono-oligonucleosomes using an ELISA kit and TUNEL assay. The production of NO, LDH, and Gpx, SOD, CAT, and Total antioxidant activities were evaluated by colorimetric assays.ResultsColloidal silver had dose-dependent cytotoxic effect in MCF-7 breast cancer cells through induction of apoptosis, shown an LD50 (3.5 ng/mL) and LD100 (14 ng/mL) (*P < 0.05), significantly decreased LDH (*P < 0.05) and significantly increased SOD (*P < 0.05) activities. However, the NO production, and Gpx, CAT, and Total antioxidant activities were not affected in MCF-7 breast cancer cells. PBMC were not altered by colloidal silver.ConclusionsThe present results showed that colloidal silver might be a potential alternative agent for human breast cancer therapy.

Green synthesis of silver nanoparticles using Ganoderma neo-japonicum Imazeki: a potential cytotoxic agent against breast cancer cells

“…AgNP-treated cells showed decreased metabolic activity, which depends on the nature of cell types and the size of NPs. 57 Franco-Molina et al 32 reported a colloidal silver-induced dose-dependent cytotoxic effect on MCF-7 breast cancer cells. The rate of cell viability was significantly reduced as a function of both culture time and AgNP concentration in human IMR-90 and U251 cells, mouse embryonic stem cells, and A549 lung cells.…”

“…For this, it is important to find alternative therapies or drugs to overcome these drawbacks. 32 Nanotechnology has contributed in several applications, such as therapeutics, 33 catalysis, 34 biosensing devices, 35 biomedicine due to its ready manufacturing process, 36 quality control, and biocompatibility, and indeed its use in wound healing and antibacterial applications are already part of clinical practice. 22,37 AgNPs have been shown to induce the apoptotic pathway in vitro through free oxygen radical generation, which showed antitumor, antiproliferative, and antiangiogenic effects in vitro.…”

“…Several cytotoxic agents have been used in the treatment of breast cancer, such as doxorubicin, cisplatin, and bleomycin, providing beneficial effects, but the efficacy and demerits are unclear. 32 Therefore, it is necessary to find novel therapeutic agents against cancer that are biocompatible and cost-effective. In this study, we firstly performed synthesis of AgNPs using G. neo-japonicum extract.…”

Biogenic silver nanoparticles mediated by Broussonetia papyrifera: anticancer and antimicrobial activity against pathogenic organisms

“…Chromatin condensation and fragmentation were majorly observed in silver nitrate, AgNPs treated cells. The colloidal Ag also inhibits the cell growth by apoptosis in MCF-7 human breast cancer cell lines [28]. The AgNPs for 48 hrs MCF-7 show the apoptotic characters, namely, cell shrinkage, nuclear condensation, and fragmentation, whereas in the control the cells exhibited nuclear architecture.…”

Antimicrobial and Anticancer Activity of Silver Nanoparticles From Edible Mushroom: A Review

“…Asharani et al [36] reported that silver nanoparticles exhibit antiproliferative effect on human glioblastoma cells. Franco-Molina et al [37] evaluated the effects of colloidal silver on [38] demonstrated that nanosilver not only disrupted normal cellular function but also affected membrane integrity, inducing various apoptotic signaling genes of mammalian cells, leading to programmed cell death. Hsin et al [39] reported that nanosilver induced apoptosis in murine embryonic fibroblast cells (NIH 3T3) by increasing the generation of reactive oxygen species and activating the c-Jun N-terminal kinase pathway, leading to mitochondria-dependent apoptosis.…”