Antitumor activity of Brazilian red propolis fractions against Hep-2 cancer cell line

Frozza, Caroline Olivieri da Silva; Santos, Denis Amilton dos; Rufatto, Luciane Corbellini; Minetto, Luciane; Scariot, Fernando Joel; Echeverrigaray, Sérgio; Pich, Claus Tröger; Moura, Sidnei; Padilha, Francine Ferreira; Borsuk, Sibele; Savegnago, Lucielli; Collares, Tiago; Seixas, Fabiana Kömmling; Dellagostin, Odir A.; Roesch‐Ely, Mariana; Henriques, João Antônio Pêgas

doi:10.1016/j.biopha.2017.05.027

Cited by 46 publications

(29 citation statements)

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“…Due to the variety of chemical compounds of BRP incorporated within the resin mixture, especially phenolic compounds, many biological activities have been reported like antioxidant [ 2 , 8 ], antibacterial [ 9 , 10 ], fungicide [ 11 ], antiparasitic [ 10 , 12 ], antineoplasic [ 13 , 14 ], anti-caries [ 15 ], cytotoxic [ 2 , 10 ], anti-inflamatory [ 16 , 17 ], antinociceptive [ 18 ] and was also beneficial to the wound healing process [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Chemical composition, immunostimulatory, cytotoxic and antiparasitic activities of the essential oil from Brazilian red propolis

et al. 2018

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Most studies of Brazilian red propolis have explored the composition and biological properties of its ethanolic extracts. In this work, we chemically extracted and characterized the essential oil of Brazilian red propolis (EOP) and assessed its adjuvant, antiparasitic and cytotoxic activities. The chemical composition of EOP was analyzed using gas chromatography with mass spectrometry (GC-MS). EOP was tested for in vitro activity against Trichomonas vaginalis (ATCC 30236 isolate); trophozoites were treated with different concentrations of EOP (ranging from 25 to 500 μg/mL) in order to establish the MIC and IC50 values. A cytotoxicity assay was performed in CHO-K1 cells submitted to different EOP concentrations. BALB/c mice were used to test the adjuvant effect of EOP. The animals were divided in 3 groups and inoculated as follows: 0.4 ng/kg BW EOP (G1); 50 μg of rCP40 protein (G2); or a combination of 0.4 ng/kg BW EOP and 50 μg of rCP40 (G3). Total IgG, IgG1 and IgG2a levels were assessed by ELISA. The major constituent compounds of EOP were methyl eugenol (13.1%), (E)-β-farnesene (2.50%), and δ-amorphene (2.3%). Exposure to EOP inhibited the growth of T. vaginalis, with an IC50 value of 100 μg/mL of EOP. An EOP concentration of 500 μg/mL was able to kill 100% of the T. vaginalis trophozoites. The EOP kinetic growth curve showed a 36% decrease in trophozoite growth after a 12 h exposure to 500 μg/mL of EOP, while complete parasite death was induced at 24 h. With regard to CHO-K1 cells, the CC50 was 266 μg/mL, and 92% cytotoxicity was observed after exposure to 500 μg/mL of EOP. Otherwise, a concentration of 200 μg/mL of EOP was able to reduce parasite proliferation by 70% and was not cytotoxic to CHO-K1 cells. As an adjuvant, a synergistic effect was observed when EOP was combined with the rCP40 protein (G3) in comparison to the administration of each component alone (G1 and G2), resulting in higher concentrations of IgG, IgG1 and IgG2a. EOP is constituted by biologically active components with promising antiparasitic and immunostimulatory activities and can be investigated for the formulation of new vaccines or trichomonacidal drugs.

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Section: Introductionmentioning

confidence: 99%

Chemical composition, immunostimulatory, cytotoxic and antiparasitic activities of the essential oil from Brazilian red propolis

et al. 2018

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“…The antiproliferative activity of the hydroethanolic extract of red propolis and two of its fractions (J and L) was evaluated in human laryngeal epidermoid carcinoma cells (Hep-2) by Da Silva Frozza et al (2017). The chemical profile of fraction J revealed the presence of formononetin, liquiritigenin, medicarpin, vestitol, isovestitol and (3S)-ferreirin, and of fraction L, only liquiritigenin.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the fractions showed a greater cytotoxic effect than the extract. Fractionation of the extract leads to a decrease in the number of chemicals present in the fraction and may increase the concentration of active compounds when compared to the exposure of cells to the crude extract (da Silva Frozza et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the antiproliferative activity of red propolis hydroalcoholic extract and its fractions obtained by partition

et al. 2020

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Abstract. Squarisi IS, De Freitas KS, Lemes DC, Ccana-Ccapatinta GV, Mejia JAA, Bastos JK, Veneziani RCS, Ambrosio SR, Tavares DC. 2018. Evaluation of the antiproliferative activity of red propolis hydroalcoholic extract and its fractions obtained by partition. Biofarmasi J Nat Prod Biochem 18: 66-69. The present study aimed to evaluate the cytotoxicity of red propolis hydroalcoholic extract (RPHE) and its fractions obtained by partition, hexanes (HF), dichloromethane (DF), ethyl acetate (AF) and n-butanol (BF), on tumor and non-tumor cell lines. For this purpose, the XTT colorimetric assay was performed on human lung fibroblasts (GM07492A, non-tumor cell), breast adenocarcinoma (MCF-7), glioblastoma (U343) and cervix adenocarcinoma (HeLa) cells. The results showed that RPHE, HF and DF presented not only cytotoxic potential to all tumor cell lines but also to normal cell line, indicating selectivity absence. HF presented the lowest IC50 (half minimal inhibitory concentration; 33.8-133.3 µg/mL), with significant difference from those observed for RPHE (137.0-262.7 µg/mL). BF and AF revealed an IC50 which was higher than 1250 µg/mL in all cell lines. The results showed that red propolis has substances with antiproliferative activity, indicating that its hexanes fraction may have substances with antitumor potential.

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“…Turkish propolis is rich from in flavonoids and phenolics such as naringenin, quercetin, pinocembrin, caffeic acid, apigenin, caffeic acid phenyl ester, pinobanksin, galangin, chrysin and cinnamic acids [9,10]. Studies have shown that many biological activities of propolis including antitumor activity may be related to its flavonoid and phenolic acid compositions [11][12][13][14]. Ethanol, methanol, water, dimethyl sulfoxide (DMSO), polyethylene glycol (PEG), oil, hexane and ethyl acetate have been used as solvents for extraction of propolis [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of cytotoxic and wound healing effect of DMEM extracts of Turkish propolis in MDA-MB-231 cell lines

Uçar¹

2019

Trop. J. Pharm Res

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Purpose: To investigate the effect of Dulbecco's Modified Eagle Medium (DMEM) extract of Turkish propolis on proliferation, cytotoxicity and lateral motility in MDA-MB-231 cells. Methods: The antiproliferative activity of DMEM extracts of propolis was determined colorimetrically in MDA-MB-231 cells using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Cell toxicity and wound healing effects of the prpolis extracts were determined with trypan blue exclusion assay and wound-healing assay, respectively. Results: The cell number of MDA-MB-231 cells were decreased by the extracts at all concentrations for 72 h. The highest antiproliferative activity of the extract was demonstrated at 10 mg/mL for 24-72 h. Moreover, 5 and 0.31 mg/mL of the propolis extract showed significant antiproliferative activity at 72 h of incubation. The extract showed cytotoxic effect to MDA-MB-231 cells at 10 mg/mL. The extract (at a dose of 2.5 mg/mL) during 24-72 h did not produce any effect on lateral motility in MDA-MB-231 cells in the wound healing assay. Conclusion: These results indicate that the DMEM extract of propolis exerts antiproliferative and cytotoxic effects on MDA-MB-231 cells at different concentrations.

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Antitumor activity of Brazilian red propolis fractions against Hep-2 cancer cell line

Cited by 46 publications

References 31 publications

Chemical composition, immunostimulatory, cytotoxic and antiparasitic activities of the essential oil from Brazilian red propolis

Chemical composition, immunostimulatory, cytotoxic and antiparasitic activities of the essential oil from Brazilian red propolis

Evaluation of the antiproliferative activity of red propolis hydroalcoholic extract and its fractions obtained by partition

Evaluation of cytotoxic and wound healing effect of DMEM extracts of Turkish propolis in MDA-MB-231 cell lines

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