Antithyroid drug detection using an enzyme cascade blocking in a nanoparticle‐based lab‐on‐a‐chip system

Kurbanoğlu, Sevinç; Mayorga‐Martinez, Carmen C.; Medina‐Sánchez, Mariana; Rivas, Lourdes; Özkan, Síbel A.; Merkoçi, Arben

doi:10.1016/j.bios.2014.10.014

Cited by 39 publications

(34 citation statements)

References 41 publications

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“…[33b, 38] It was demonstrated recentlyt hat enzymecoated MNPs can be fixed at ac ertain positiono faLab-on-a-Chip (LoC) system by using ap ermanent magnet. [39] To the best of our knowledge,n om icrofluidic system has exploited multiple magnetic cells-which we here term "Magne-Chip"for biotransformation, analysis, or protein biochemistry studies ( Figure 1).…”

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confidence: 99%

Phenylalanine Ammonia‐Lyase‐Catalyzed Deamination of an Acyclic Amino Acid: Enzyme Mechanistic Studies Aided by a Novel Microreactor Filled with Magnetic Nanoparticles

et al. 2015

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Phenylalanine ammonia-lyase (PAL), found in many organisms, catalyzes the deamination of l-phenylalanine (Phe) to (E)-cinnamate by the aid of its MIO prosthetic group. By using PAL immobilized on magnetic nanoparticles and fixed in a microfluidic reactor with an in-line UV detector, we demonstrated that PAL can catalyze ammonia elimination from the acyclic propargylglycine (PG) to yield (E)-pent-2-ene-4-ynoate. This highlights new opportunities to extend MIO enzymes towards acyclic substrates. As PG is acyclic, its deamination cannot involve a Friedel-Crafts-type attack at an aromatic ring. The reversibility of the PAL reaction, demonstrated by the ammonia addition to (E)-pent-2-ene-4-ynoate yielding enantiopure l-PG, contradicts the proposed highly exothermic single-step mechanism. Computations with the QM/MM models of the N-MIO intermediates from L-PG and L-Phe in PAL show similar arrangements within the active site, thus supporting a mechanism via the N-MIO intermediate.

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confidence: 99%

Phenylalanine Ammonia‐Lyase‐Catalyzed Deamination of an Acyclic Amino Acid: Enzyme Mechanistic Studies Aided by a Novel Microreactor Filled with Magnetic Nanoparticles

et al. 2015

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“…[36] In all the aforementioned papers, very low LODs (� 0.004 μmol L À 1 ) were obtained for the analysis of pesticides in natural water samples. In addition, in 2015, FIA-SPE methods were also explored for the determination of analytes such as methimazole, [17] nimodipine, [37] biotin, [15] pioglitazone, [38] oxicams derivatives, [39] H 2 O 2 , [14,40] nitromethane, [41] glucose, [42] 3hydroxyanthranilic acid, [43] bromate, [44] phosphate through monitoring of an electroactive complex obtained by the reaction between phosphate and molybdate, [45] and iodide. [46] During the year 2016, the possibility of determination of free chlorine, [47] hydroquinone, [48] labile copper, [49] Cr(VI), [50] and zinc phenolsulfonate [24] were reported.…”

Section: Spes Coupled To Fia Systemsmentioning

confidence: 99%

“…Over recent years, many research groups have successfully demonstrated that homemade flowcells with different designs can be constructed and used for the positioning of SPEs. Flow-cells for FIA, [13][14][15][16][17][18] BIA, [19][20][21] and HPLC [22] systems are available in the literature. However, to attract potential new users, it is very important that a robust and easy to use flow systems for SPEs are commercially available.…”

Section: Introductionmentioning

confidence: 99%

An Overview of Recent Electroanalytical Applications Utilizing Screen‐Printed Electrodes Within Flow Systems

et al. 2020

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This review addresses the use of screen-printed electrodes (SPEs) coupled to flow systems such as flow injection analysis (FIA), batch injection analysis (BIA), and high-performance liquid chromatography (HPLC) systems over the last six years (since 2014). The combination of SPEs and flow systems is a powerful synergy to increase the throughput of measurements, improve electrode lifetime, and reduce reagent consumption and waste generation. Recently, commercial flow cells for SPEs were made available by different companies and potential new users that are unable to construct homemade electrochemical flow cells have been attracted to work in the area. This overview aims to show both trends and future potential for the development of new methods useful for modern electroanalysis and/or portable applications.

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“…8 With this aim, polythinoine layers have been reported to be used for proper modifications of sensors. 19,20 The platform developed shows good performance for electrochemical sensing of PCR amplified DNA of dog affected by Leishmania, this target is used as a proof a concept. [11][12][13][14] However, various issues like poor compatibility of immobilizing materials on the transducer, poor binding of DNA, creation of inactive or poor conducting layers and less surface area including poor stability were raised.…”

Section: Introductionmentioning

confidence: 99%

An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

et al. 2015

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An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO 2 NP). An aminated oligonucleotide probe is immobilized on the IrO 2 NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD E 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.Scheme 1 Schematic representation of the procedure for the impedimetric label-free genosenor fabrication. Thionine electropolymerization (a), deposition of IrO 2 NPs (b), immobilization of ss-DNA (c), blocking step with etanolamine (d) and hybridization with a complementary target (e).

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Antithyroid drug detection using an enzyme cascade blocking in a nanoparticle‐based lab‐on‐a‐chip system

Cited by 39 publications

References 41 publications

Phenylalanine Ammonia‐Lyase‐Catalyzed Deamination of an Acyclic Amino Acid: Enzyme Mechanistic Studies Aided by a Novel Microreactor Filled with Magnetic Nanoparticles

Phenylalanine Ammonia‐Lyase‐Catalyzed Deamination of an Acyclic Amino Acid: Enzyme Mechanistic Studies Aided by a Novel Microreactor Filled with Magnetic Nanoparticles

An Overview of Recent Electroanalytical Applications Utilizing Screen‐Printed Electrodes Within Flow Systems

An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

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