Antithrombin Iii and the Œstrogen Content of Combined Œstro-Progestagen Contraceptives

Conard, J.; Samama, M; Salomon, Y

doi:10.1016/s0140-6736(72)92757-2

Cited by 55 publications

(15 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In any case our findings do not support previous reports of reduced AT-III levels during OC treatment [6,8,10,11,14,16]. It must be stressed, however, that, in earlier reports, the lowest AT-III values were found during high-level OC treatment and with assays performed on serum (table I).…”

Section: Discussioncontrasting

confidence: 99%

Thrombin Inhibitors in Women on Oral Contraceptives

Guagnellini¹,

Bertolini²,

Cappelletti³

et al. 1981

Acta Haematol

View full text Add to dashboard Cite

Progressive antithrombin activity and the immunological levels of antithrombin III, α₂-macroglobulin, and α₁-antitrypsin were measured in women at various intervals during treatment with oral contraceptives and in a group of untreated women. No significant changes were observed in any of the above parameters. In both control and treated women, there was a positive statistical correlation between progressive antithrombin activity and antithrombin III immunological levels, whereas no correlation was found between the former and α₁-antitrypsin or α₂-macroglobulin immunological levels. This study questions the possibility of thrombin inhibitors being selective signs of hypercoagulability during treatment with oral contraceptives.

show abstract

Section: Discussioncontrasting

confidence: 99%

Thrombin Inhibitors in Women on Oral Contraceptives

Guagnellini¹,

Bertolini²,

Cappelletti³

et al. 1981

Acta Haematol

View full text Add to dashboard Cite

show abstract

“…Although many are the physiological in hibitors of thrombin, it is widely accepted that AT III is the most important one, its antiproteasic action being also directed to wards several other serine proteases involved in the haemostatic mechanism, as factor IXa, Xa, XIa [15,23], A correlation between reduced plasma levels of AT III and predisposition to throm boembolic events has been proved in several acquired conditions, such as oestrogenic treatment [8,11,24,34], post-operative states [1,13,30], and nephrotic syndrome [31]. However, the most convincing evidence for the role of AT III deficiency in causing thrombotic tendency comes from studies of families with congenital defect.…”

Section: Discussionmentioning

confidence: 99%

Inherited Deficiency of Antithrombin III in Two Italian Families

Manotti¹,

Quintavalla

Megha

et al. 1982

Pathophysiol Haemos Thromb

View full text Add to dashboard Cite

A significant decrease of plasma antithrombin III (AT III) levels, measured with four different biological and immunological methods, was found in 8 of 11 members of a Sicilian family (family DM) and in 3 of 14 members of a northern Italian family (family A). Different behaviour after oral anticoagulant treatment with acenocoumarin was seen in 2 long-term treated subjects. The propositus of family DM, who had had a long history of recurrent thrombosis, did not show any increase of AT III levels. A significant increase was, on the contrary, observed in the propositus of family A, who had suffered a recent thrombosis in a branch of the inferior mesenteric vein.

show abstract

“…reported to occur as a result of heparin ther apy [24], in connection with the use of oral contraceptives [9,20,39] and as a conse quence of pregnancy [15], surgery [31], liver cirrhosis [4] or malignancies involving the liver [2]. AT III concentrate has shown a protective effect against intravenous injec tion of thromboplastin in chicken embryos [21] and in rats [27], and recent clinical trials have demonstrated its therapeutic ef fect against disseminated intravascular co agulation in humans [2,32], Several investigators have reported the isolation of AT III from human plasma by conventional purification techniques, but the procedures were lengthy and the yields were poor [1,18,30], A substantial im provement in the AT III purification meth odology took place when insolubilized hep arin was introduced as an affinity adsorbent [25,37], but the methods described include small scale purification steps such as column chromatography and gel filtration on Sephadex G-200.…”

Section: Methodsmentioning

confidence: 99%

Development of Large Scale Fractionation Methods

Wickerhauser¹,

Williams²,

Mercer³

1979

Vox Sanguinis

View full text Add to dashboard Cite

A large scale method for preparation of antithrombin III (AT III) concentrate from plasma or from Cohn fraction IV-1 (Fr. IV-1) has been described. It consists of the following steps: (a) partial purification by precipitation of impurities with 20% polyethylene glycol (PEG) 4000; (b) isolation of AT III from the PEG supernatant by batch adsorption and elution on heparin-Sepharose at a ratio corresponding to 45 voi of plasma or 80 voi of 10% Fr. IV-1 solution to 1 voi of gel; (c) concentration and desalting of the eluted AT III on a Pellicon ultrafiltration system; (d) pasteurization of AT III concentrate by heating for 10 h at 60 °C in the presence of 0.5 M sodium citrate at pH 7.5; (e) removal of excess citrate by gel filtration on Sephadex G-50; and (f) sterile filtration, filling and lyophilization. The recovery by activity was 32% from a 113-liter plasma batch and 16% from a 42-kg Fr. IV-1 batch. Both AT III concentrates, derived either from plasma or from Fr. IV-1, had similar specific activity and electrophoretic purity, were nonpyrogenic and met all other FDA requirements for biologic products. Pasteurization induced changes in disc gel and isotachophoretic patterns of AT III preparations.

show abstract

Antithrombin Iii and the Œstrogen Content of Combined Œstro-Progestagen Contraceptives

Cited by 55 publications

References 6 publications

Thrombin Inhibitors in Women on Oral Contraceptives

Thrombin Inhibitors in Women on Oral Contraceptives

Inherited Deficiency of Antithrombin III in Two Italian Families

Development of Large Scale Fractionation Methods

Contact Info

Product

Resources

About