Antispermatogenic activity of 1-p. Chlorobenzyl-1H indazol-3-carboxylic acid (AF ) in rats

C, De Martino; Stefanini, Mario; A, Agrestini; Cocchia, Domenico; Morelli, Maria Beatrice; Ps, Barcellona

doi:10.1016/0014-4800(75)90028-3

Cited by 48 publications

(7 citation statements)

References 40 publications

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“…animals (Chapin et al -1983;Del Conte -1975;De Martino -1975;Hunt and Johnson -1971;OettlC and Harrison -1952;Rao and Srivastava -1967;Tso and Lacy -1979;Wong and Hruban -1972) spermatid giant cells could be described after the application of various exogenous noxes. Yet these Discussion ones.…”

Section: Multinucleated Spennatidsmentioning

confidence: 99%

“…The light microscopic picture based on paraffin sections of the spermatid giant cells is well known, since Maximow -1899; Mills -1919; Eissner -1973 described them in single observations as well as in systematic investigation. In series of experimental studies in From all the multinucleated germ cells spermatid giant cells are the most prominent animals (Chapin et al -1983;Del Conte -1975;De Martino -1975;Hunt and Johnson -1971;OettlC and Harrison -1952;Rao and Srivastava -1967;Tso and Lacy -1979;Wong and Hruban -1972) spermatid giant cells could be described after the application of various exogenous noxes. Human spermatid giant cells are extraordinarily large and contain many cell nuclei, which are arranged around a new cytocentrum like a wreath.…”

Section: Multinucleated Spennatidsmentioning

confidence: 99%

“…In the literature spermatid Multinucleated primary spermatocytes and spermatids are found in human seminiferous giant cells are described as a sign of degenerative processes in seminiferous tubules resulting from various external noxes. Experimental studies in animals have repeatedly pointed to the origin of these cells (Chapin et al -1983;Del Conte -1975;De Martino -1975;Hunt and Johnson -1971;Oettle and Harrison -1952;Rao and Srivastava -1967;Tso and Lacy -1979;Wong and Hruban -1972). The authors mainly described defects of the intercellular bridges.…”

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confidence: 99%

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Multinucleated Spermatocytes and Spermatids in Human Seminiferous Tubules*

Holstein¹,

Eckmann²

2009

Andrologia

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Thd cytology of multinucleated spermatocytes and spermatid giant cells in the seminiferous tubules of men with oligozoospermia and of men older than 65 years has been investigated electron microscopically. Two different processes which are responsible for the origin of multinucleated germ cells have been analysed: 1. Defects of the intercellular bridges (IB): they move apart and thus allow the confluence of one clone's cells to symplasms. 2. The confluence of membranes: within one clone the membranes of neighbouring germ cells are dissolved and thus intercellular bridges are found in the cytoplasm of the resulting giant cells. The spermatid giant cells reveal a new organization of the cell nuclei and the cell organelles. Yet they disintegrate in the lumen of the seminiferous tubules. The appearance of giant cells therefore is an expression of the germ cell degeneration. Vielkernige Sperrnatozyten und Spermatiden in menschlichen SarnenkanalchenZusammenfassung: Die Zytologie von mehrkernigen Spermatozyten und Spermatidenriesenzellen in Samenkandchen von Mannern mit Oligozoospermie und bei Mannern, die iilter als 65 Jahre sind, wird elektronenmikroskopisch untersucht. Fur das Zustandekommen der mehrkemigen Keimzellen lassen sich zwei Vorgange erkennen: 1. Defekte der Interzellularbriicken (IB): sie weichen auseinander und die Zellen eines Klon fliefkn zu Symplasmen zusammen. 2. Das Zusammenfliefkn benachbarter Zellmembranen der Zellen eines Keimzellklon: dadurch werden die Interzellularbriicken in das Zytoplasma der so entstandenen Riesenzelle verlagert. Die Spennatidenriesemllen zeigen eine neue Organisation der Zellkerne und Zellorganellen, gehen letztlich aber zugrunde. Das Auftreten der Riesenzellen ist ein Ausdruck der Degeneration von Keimzellen.* Dedicated to Prof. Dr. Edward Roosen-Runge, Seattle, at the occasion of his 75th birthday.

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Section: Multinucleated Spennatidsmentioning

confidence: 99%

Section: Multinucleated Spennatidsmentioning

confidence: 99%

mentioning

confidence: 99%

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Multinucleated Spermatocytes and Spermatids in Human Seminiferous Tubules*

Holstein¹,

Eckmann²

2009

Andrologia

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“…Studies of the effects of 1H-indazole-3-carboxylic acid in the testis first began when its derivative, 1-p-chlorobenzyl-1H-indazole-3-carboxylic acid (AF-1312/TS), was shown to possess antispermatogenic activity Coulston et al 1975;De Martino et al 1975;Silvestrini et al 1975). …”

Section: Introductionmentioning

confidence: 99%

“…Early studies suggested that the Sertoli cell is the primary target of AF-1312/TS and lonidamine (De Martino et al 1975Lobl 1979). However, it was found that AF-1312/TS and lonidamine also exerted a direct effect on altering the energy metabolism of germ cells (Floridi et al 1981a,b) but not Sertoli cells .…”

Section: Introductionmentioning

confidence: 99%

Cell Junctions in the Testis as Targets for Toxicants

Wong

Yan

et al. 2010

Comprehensive Toxicology

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The Effects of the Indazole Carboxylic Acid Derivative, Tolnidamine, on Testicular Function: I

et al. 1985

Journal of Andrology

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The indazole carboxylic acid derivative, tolnidamine, has marked antispermatogenic activity in several animal species. In this study, we assessed the effect of tolnidamine on rat Sertoli cell function both in vivo and in vitro, using androgen binding protein (rABP) as a marker. Groups of six male rats were killed 2, 4, 8, 16, 32, 64 hours and 5, 8, and 12 days following tolnidamine administration (250 mg/kg by oral gavage). There was a progressive reduction in both testicular and epididymal weights. Serum FSH levels did not change and LH showed a transient increase between 64 hours and 8 days. Except for an initial increase at 2 hours, there were no changes in serum testosterone. Epididymal rABP concentration and content declined as early as 8 hours, with the lowest values occurring at 5 and 12 days. By 16 hours, there was an increase in testicular rABP, which was also evident at 8 days and 12 days. Within 16 hours after tolnidamine, there was a rise in serum rABP, which persisted until the end of the experiment. When another indazole carboxylic acid derivative, lonidamine, was administered (250 mg/kg), similar changes were evident in epididymal and serum rABP at 32 hours, but the rapid decrease in testicular rABP suggested a different mechanism of action. In another experiment, single oral doses of tolnidamine (50, 100, 250, and 500 mg/kg) were administered to other groups of rats and the animals were killed after 24 hours and 5 days. With increasing doses of tolnidamine, there was a reduction in epididymal rABP concomitant with an increase in testis and serum rABP levels.(ABSTRACT TRUNCATED AT 250 WORDS)

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Antispermatogenic activity of 1-p. Chlorobenzyl-1H indazol-3-carboxylic acid (AF ) in rats

Cited by 48 publications

References 40 publications

Multinucleated Spermatocytes and Spermatids in Human Seminiferous Tubules*

Multinucleated Spermatocytes and Spermatids in Human Seminiferous Tubules*

Cell Junctions in the Testis as Targets for Toxicants

The Effects of the Indazole Carboxylic Acid Derivative, Tolnidamine, on Testicular Function: I

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