Antisperm Antibodies in Prepubertal Boys and Their Reactivity with Antigenic Determinants on Differentiated Spermatozoa

Domagala, Alina; Kamieniczna, Marzena; Kowalczyk, Dariusz W.; Kurpisz, Maciej

doi:10.1111/j.1600-0897.1998.tb00416.x

Cited by 14 publications

(11 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous study, we detected ASA in 7% of prepubertal boys (n ¼ 69) with testicular failure (mainly uni-and bilateral cryptorchidism) by means of IDIBT [2]. In the same study, we also detected ASA in some healthy prepubertal boys.…”

Section: Introductionmentioning

confidence: 51%

“…For each class of immunoglobulin, at least 200 sperm cells were counted and the percentage of positive ones was calculated. A result was considered as positive when at least 10% of sperm cells were attached to the beads [2] at a lower cut-off. Topography of immunobead binding to the particular region of a sperm cell (head, midpiece, tail) was also observed.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Antisperm Antibodies in Prepubertal Boys With Cryptorchidism

Domagala

Havryluk

Kamieniczna

et al. 2006

Archives of Andrology

View full text Add to dashboard Cite

The presence of antisperm antibodies in male individuals before puberty is controversial due to the lack of finally differentiated male germ cells. It was questioned whether the pathologic conditions of the male gonad may influence antisperm antibody formation in individuals before puberty. Sera samples of 76 individuals and 10 healthy boys with testicular failure (mainly uni- or bilateral cryptorchidism) were examined by means of indirect immunobead-binding test (IDIBT). The presence of antisperm antibodies was found in 3.95% of the studied subjects. Antibodies recognizing antigenic determinants present on the surface of mature sperm cells may be produced before puberty in individuals suffering from cryptorchidism or the other gonadal disorders. Antisperm antibodies that did develop in a minority of the studied male population may be proof for individual predispositions to autoimmune reactions.

show abstract

Section: Introductionmentioning

confidence: 51%

Section: Methodsmentioning

confidence: 99%

Antisperm Antibodies in Prepubertal Boys With Cryptorchidism

Domagala

Havryluk

Kamieniczna

et al. 2006

Archives of Andrology

View full text Add to dashboard Cite

show abstract

“…More than 70% of men develop ASAs after vasectomy (17), and there is a limited success in the regain of fertility, even after successful surgical reanastomosis in vasovasostomy attributed to the presence of ASAs (18). Up to 2% to 30% cases of infertility may be associated with the presence of ASAs in the male or female partner of an infertile couple (19,20). These findings provided evidence that spermatozoa can generate an immune response in both men and women, which can lead to a contraceptive state.…”

Section: Discussionmentioning

confidence: 98%

In vitro and in vivo studies evaluating antisemen antibodies as a potential spermicidal agent in hamsters

Chen

Liu

Xie

et al. 2009

Fertility and Sterility

View full text Add to dashboard Cite

“…The procedure has been previously described [10]. Briefly, motile sperm cells were isolated from semen of AsA-negative donors by swim-up technique.…”

Section: Methodsmentioning

confidence: 99%

Weak association of anti-sperm antibodies and strong association of familial cryptorchidism/infertility with HLA-DRB1polymorphisms in prepubertal Ukrainian boys

Kurpisz

Nakonechnyy

Niepiekło-Miniewska

et al. 2011

Reprod Biol Endocrinol

View full text Add to dashboard Cite

BackgroundCryptorchidism is a frequent syndrome occurring in 1-2% of males within the first year of age. Autoimmune reactions, particularly directed to testicular elements and/or spermatozoa have been found to be often associated with cryptorchidism. Therefore we investigated in this study the frequency of HLA class II alleles in order to recognize possible genetic predisposition for antisperm antibodies development in prepubertal boys with diagnosed cryptorchidism in Caucasoid population.MethodsSixty prepubertal boys with cryptorchidism and sixty healthy boys were examined for anti-sperm antibodies by indirect immunobead test as well as for their HLA-DRB1 and -DQB1 alleles using DNA obtained from peripheral blood leukocytes. The typing of HLA-DRB1 and -DQB1 was performed by using PCR-SSP low resolution method.ResultsAllele frequencies of HLA-DRB1 and HLA-DQB1 did not differ between boys with cryptorchidism and control boys. However, weakly significant differences in DRB1*04 (p corrected = 0.0475) and DQB1*06 (p corrected = 0.0385) were seen between cryptorchid patients with and without AsA, but none of these two patient groups differed significantly in HLA class II frequencies from controls except for AsA-negatives and HLA-DQB1*06 (p corrected = 0.0247). On the other hand, comparison of cryptorchid boys with familial cryptorchidism and/or infertility to control boys revealed highly significant (p corrected = 0.0006) difference in HLA-DRB*11 frequency, whereas boys with sporadic cryptorchidism did not differ from control. A much weaker, but still significant difference in DRB*11 frequency was also observed between boys with bilateral cryptorchidism and controls (p corrected = 0.037), whereas patients with unilateral cryptorchidism were not different from control in frequency of any HLA-DRB1 or -DQB1 allele tested.ConclusionsPredisposition to produce anti-sperm antibodies seems to be only weakly associated with HLA class II genes, although this question requires further study on much larger population sample. It is plausible that familial and sporadic cryptorchidism may present distinct genetic background. The same may, to lower extent, apply to bilateral and unilateral cryptorchidism.

show abstract

Antisperm Antibodies in Prepubertal Boys and Their Reactivity with Antigenic Determinants on Differentiated Spermatozoa

Cited by 14 publications

References 17 publications

Antisperm Antibodies in Prepubertal Boys With Cryptorchidism

Antisperm Antibodies in Prepubertal Boys With Cryptorchidism

In vitro and in vivo studies evaluating antisemen antibodies as a potential spermicidal agent in hamsters

Weak association of anti-sperm antibodies and strong association of familial cryptorchidism/infertility with HLA-DRB1polymorphisms in prepubertal Ukrainian boys

Contact Info

Product

Resources

About