Antisense Technology

Zhang, Ruiwen; Wang, Hui

doi:10.1007/978-1-59259-785-7_4

Cited by 18 publications

(2 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DOI: https://doi.org/10.37191/Mapsci-JCGH-1(1)-002B. Highly Specific Mechanism of Action:Antisense oligonucleotides have been incorporated into gene therapy due to their high specificity, low toxicity, and high efficiency[115][116][117][118].…”

mentioning

confidence: 99%

Investigating the Frontiers of Genetic Regulation and Unlocking the Secrets of Gene Expression: The Power of RNA-based Therapeutics

HR¹

2023

J Clin Genet Hered.

View full text Add to dashboard Cite

Advancements in genome editing have revolutionized the field of biotechnology by enabling precise manipulation of DNA sequences. The ability to edit genes has significant implications for treating genetic diseases and developing novel therapeutics. Despite the considerable progress made in genome editing, there are still concerns over its off-target effects and ethical considerations, which have spurred the exploration of alternative approaches. In this context, we present a paper on antisense technology, an innovative biological approach that has the potential to regulate gene expression by halting the translation process of mRNA and suppressing protein production. We highlight the limitations of current genome editing technologies and the need for more targeted and personalized approaches to treat genetic disorders. Antisense technology employs artificially synthesized oligonucleotides or small RNA sequences to target specific genes and inhibit their expression. This approach provides greater specificity and control over gene expression, making it a highly promising therapeutic option for various diseases such as cancer, cardiovascular diseases, and viral infections. We discuss the current status of antisense technology, its potential future prospects, as well as the challenges and opportunities that need to be addressed to fully exploit its potential. Overall, our paper aims to shed light on the significance of antisense technology in the field of biotechnology and its potential to revolutionize gene expression regulation. By providing an overview of this innovative approach, we hope to inspire further research in this area and pave the way for novel and more effective therapies for genetic disorders.

show abstract

mentioning

confidence: 99%

Investigating the Frontiers of Genetic Regulation and Unlocking the Secrets of Gene Expression: The Power of RNA-based Therapeutics

HR¹

2023

J Clin Genet Hered.

View full text Add to dashboard Cite

show abstract

“…First discovered around 1980, these naturally occurring single stranded oligonucleotides are complementary to the RNA sequence of their target. Synthetic antisense drugs have been created to inhibit the expression of a gene through binding to the targeted sequence, inhibiting translation of the gene (Zhang, 2005). While this gene-specific approach aims to improve upon whole cell effects by only inhibiting the undesired gene, it fails to exclusively target cancer cells, exhibiting highly toxic side effects in all cells.…”

Section: Oligonucleotide Therapymentioning

confidence: 99%

The VEGF quadruplex-forming sequence inhibits lung cancer cell growth.

Muench

View full text Add to dashboard Cite

ACKNOWLEDGEMENTSFirst off, I would like to thank Dr. Donald Miller for taking me on as a co-op student and introducing me to the vitally important and fascinating world of cancer research. He not only helped me discover my dream job, but he has also set an outstanding example of how to be an extraordinary physician scientist that I one day hope to take after. I also want to thank all of the members of the Miller/Bates lab who have helped me learn how fun life can be as a scientist and have inspired me to keep improving no matter what. Thank you to Sheila Thomas for her help with the stability experiments, and to Dr. Tariq Malik for his help with the in vivo imaging studies. Special thanks to Dr. Kara Sedoris, who had the patience and fortitude to work with me every day. I enjoyed every minute of it, and couldn't have done it without her! I also want to thank Dr. Frieboes and Dr. Panchapakesan for being part of my committee. I appreciate all of your hard work and advice which has been extremely helpful. I also want to thank Dr. Andre Gobin for being such a great professor and for sponsoring my independent study, which was a very enjoyable experience! To determine the biological role of VEGFq on non-small cell lung cancer in vivo, A549 cells were injected into nude mice and grown for 10 days prior to daily IP injections of 10 mg/kg VEGFq or the control vehicle for 14 days. Tumor progression was physically measured using calipers three times a week. Fluorescent imaging was used to detect the presence, stability, and distribution of Alexa Fluor 750-labeled VEGFq after injection into the mouse to ensure localization in the targeted tumor.These results demonstrate that A549 cells treated with VEGF quadruplex-forming oligonucleotides experience a dramatic decrease in cell proliferation, suggesting that VEGFq may have significant therapeutic implications for the treatment of non-small cell lung cancer.

show abstract

Control of Gene Expression by RNAi: A Revolution in Functional Genomics

Kumar

Salar

2017

Plant Biotechnology: Recent Advancements and Developments

View full text Add to dashboard Cite

Antisense Technology

Cited by 18 publications

References 53 publications

Investigating the Frontiers of Genetic Regulation and Unlocking the Secrets of Gene Expression: The Power of RNA-based Therapeutics

Investigating the Frontiers of Genetic Regulation and Unlocking the Secrets of Gene Expression: The Power of RNA-based Therapeutics

The VEGF quadruplex-forming sequence inhibits lung cancer cell growth.

Control of Gene Expression by RNAi: A Revolution in Functional Genomics

Contact Info

Product

Resources

About