Control of Gene Expression by RNAi: A Revolution in Functional Genomics

Kumar, Suresh; Salar, Raj Kumar

doi:10.1007/978-981-10-4732-9_2

Cited by 2 publications

(1 citation statement)

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“…In plants, these pathways have a role in resistance to pathogens (Rosa et al, 2018a) and are also required for normal development (Carrington and Ambros, 2003). The use of RNAi to silence specific genes has been a valuable tool in plant functional genomics (McGinnis, 2010, Kumar andSalar, 2017). There have also been a number of applied biotechnology applications of RNAi (Frizzi and Huang, 2010).…”

Section: Introductionmentioning

confidence: 99%

Carbon dots for efficient siRNA delivery and gene silencing in plants

Schwartz

Hendrix

Hoffer

et al. 2019

Preprint

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The Initiation of RNA interference (RNAi) by topically applied double stranded RNA (dsRNA) has potential applications for plant functional genomics, crop improvement and crop protection. The primary obstacle for the development of this technology is efficient delivery of RNAi effectors. The plant cell wall is a particularly challenging barrier to the delivery of macromolecules. Many of the transfection agents that are commonly used with animal cells produce nanocomplexes that are significantly larger than the size exclusion limit of the plant cell wall. Utilizing a class of very small nanoparticles called carbon dots, a method of delivering siRNA into the model plant Nicotiana benthamiana and tomato is described. Low-pressure spray application of these formulations with a spreading surfactant resulted in strong silencing of GFP transgenes in both species. The delivery efficacy of carbon dot formulations was also demonstrated by silencing endogenous genes that encode two sub-units of magnesium chelatase, an enzyme necessary for chlorophyll synthesis. The strong visible phenotypes observed with the carbon dot facilitated delivery were validated by measuring significant reductions in the target gene transcript and/or protein levels. Methods for the delivery of RNAi effectors into plants, such as the carbon dot formulations described here, could become valuable tools for gene silencing in plants with practical applications in plant functional genomics and agriculture.

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