Antisense oligonucleotide technology can be used to investigate a circular but not linear RNA-mediated function for its encoded gene locus

Song, Zhenxing; Jia, Ruirui; Tang, Ming‐Chun; Xia, Fei; Xu, Haiyang; Li, Zhengguo; Huang, Chuan

doi:10.1007/s11427-020-1743-8

Cited by 17 publications

(12 citation statements)

References 59 publications

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“…Cellular fractionation of S2 cells was performed as described in our previous works ( 35 , 37 , 38 ). In brief, after RNAi and/or metal treatment, cells were washed twice with 1 ml of 1× PBS and resuspended with slow pipetting in 1 ml of ice-cold lysis buffer A (10 mM Tris–HCl pH 8, 140 mM NaCl, 1.5 mM MgCl 2 , 0.5% IGEPAL CA-630 (v/v) and 1 mM dithiothreitol).…”

Section: Methodsmentioning

confidence: 99%

Gawky modulates MTF-1-mediated transcription activation and metal discrimination

Jia

Song

Lin

et al. 2021

Nucleic Acids Research

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Metal-induced genes are usually transcribed at relatively low levels under normal conditions and are rapidly activated by heavy metal stress. Many of these genes respond preferentially to specific metal-stressed conditions. However, the mechanism by which the general transcription machinery discriminates metal stress from normal conditions and the regulation of MTF-1-meditated metal discrimination are poorly characterized. Using a focused RNAi screening in Drosophila Schneider 2 (S2) cells, we identified a novel activator, the Drosophila gawky, of metal-responsive genes. Depletion of gawky has almost no effect on the basal transcription of the metallothionein (MT) genes, but impairs the metal-induced transcription by inducing the dissociation of MTF-1 from the MT promoters and the deficient nuclear import of MTF-1 under metal-stressed conditions. This suggests that gawky serves as a ‘checkpoint’ for metal stress and metal-induced transcription. In fact, regular mRNAs are converted into gawky-controlled transcripts if expressed under the control of a metal-responsive promoter, suggesting that whether transcription undergoes gawky-mediated regulation is encrypted therein. Additionally, lack of gawky eliminates the DNA binding bias of MTF-1 and the transcription preference of metal-specific genes. This suggests a combinatorial control of metal discrimination by gawky, MTF-1, and MTF-1 binding sites.

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Section: Methodsmentioning

confidence: 99%

Gawky modulates MTF-1-mediated transcription activation and metal discrimination

Jia

Song

Lin

et al. 2021

Nucleic Acids Research

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“…During the production of a mature mRNA, introns must be removed and exons are joined together in a linear order, which is known as canonical splicing [ [139] , [140] , [141] ]. Alternatively, splicing events can also occurs in a way that a splicing donor is joined to an upstream acceptor, thereby generating a covalently-closed RNA (circular RNA) [ 7 , [142] , [143] , [144] , [145] ]. Despite the first circular RNA being discovered in 1976 [ 146 ], only recently has it been appreciated that these covalently-closed molecules represent a common class of transcriptional outputs from numerous protein-coding genes in the eukaryotic system [ 147 , 148 ].…”

Section: Nuclear Circular Rnamentioning

confidence: 99%

The nuclear functions of long noncoding RNAs come into focus

Song

Lin

et al. 2021

Non-coding RNA Research

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Long noncoding RNAs (lncRNAs), defined as untranslated and tightly-regulated transcripts with a length exceeding 200 nt, are common outputs of the eukaryotic genome. It is becoming increasingly apparent that many lncRNAs likely serve as important regulators in a variety of biological processes. In particular, some of them accumulate in the nucleus and function in diverse nuclear events, including chromatin remodeling, transcriptional regulation, RNA processing, DNA damage repair, etc. Here, we unite recent progresses on the functions of nuclear lncRNAs and provide insights into the future research directions of this field.

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“…ASOs have been not only broadly used in protein or RNA biology 51 , but also served as a highly promising therapeutic strategy for many diseases 52 - 54 . Recent study has shown that ASOs can be used to investigate a circular but not linear RNA-mediated function for its encoded gene locus 55 , which makes back-splicing-junction (BSJ) targeting-ASOs a highly promising and specific strategy for circRNA targeting.…”

Section: Introductionmentioning

confidence: 99%

Estrogen-induced circRNA, circPGR, functions as a ceRNA to promote estrogen receptor-positive breast cancer cell growth by regulating cell cycle-related genes

Wang

Jia

et al. 2021

Theranostics

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Antisense oligonucleotide technology can be used to investigate a circular but not linear RNA-mediated function for its encoded gene locus

Cited by 17 publications

References 59 publications

Gawky modulates MTF-1-mediated transcription activation and metal discrimination

Gawky modulates MTF-1-mediated transcription activation and metal discrimination

The nuclear functions of long noncoding RNAs come into focus

Estrogen-induced circRNA, circPGR, functions as a ceRNA to promote estrogen receptor-positive breast cancer cell growth by regulating cell cycle-related genes

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