Antisecretory, Gastroprotective, Antioxidant and Anti-Helicobcter Pylori Activity of Zerumbone from Zingiber Zerumbet (L.) Smith

Abstract

Background Zingiber zerumbet Smith is a perennial herb, broadly distributed in many tropical areas. In Malaysia, it’s locally known among the Malay people as “lempoyang” and its rhizomes, particularly, is widely used in traditional medicine for the treatment of peptic ulcer disease beyond other gastric disorders.Aim of the studyThe aim of the current study is to evaluate the gastroprotective effect of zerumbone, the main bioactive compound of Zingiber zerumbet rhizome, against ethanol-induced gastric ulcer model in rats.Materials and MethodsRats were pre-treated with zerumbone and subsequently exposed to acute gastric ulcer induced by absolute ethanol administration. Following treatment, gastric juice acidity, ulcer index, mucus content, histological analysis (HE and PAS), immunohistochemical localization for HSP-70, prostaglandin E2 synthesis (PGE2), non-protein sulfhydryl gastric content (NP-SH), reduced glutathione level (GSH), and malondialdehyde level (MDA) were evaluated in ethanol-induced ulcer in vivo. Ferric reducing antioxidant power assay (FRAP) and anti-H. pylori activity were investigated in vitro.ResultsThe results showed that the intragastric administration of zerumbone protected the gastric mucosa from the aggressive effect of ethanol-induced gastric ulcer, coincided with reduced submucosal edema and leukocyte infiltration. This observed gastroprotective effect of zerumbone was accompanied with a significant (p <0.05) effect of the compound to restore the lowered NP-SH and GSH levels, and to reduce the elevated MDA level into the gastric homogenate. Moreover, the compound induced HSP-70 up-regulation into the gastric tissue. Furthermore, zerumbone significantly (p <0.05) enhanced mucus production, showed intense PAS stain and maintained PG content near to the normal level. The compound exhibited antisecretory activity and an interesting minimum inhibitory concentration (MIC) against H. pylori strain.ConclusionThe results of the present study revealed that zerumbone promotes ulcer protection, which might be attributed to the maintenance of mucus integrity, antioxidant activity, and HSP-70 induction. Zerumbone also exhibited antibacterial action against H. pylori.

RETRACTED ARTICLE: The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats

“…Several medicinal plants have been used to protect the stomach from a number of ulcerative agents. One type of medicinal plant is C. barometz leaves, which are used to stop bleeding [13]. In this study, the results of acute toxicity experiment revealed no signs of toxicity or mortality in vivo and no variations in kidney and liver on biochemical investigation.…”

“…Cibotium is a tropical native medicinal plant in the Malaysian Peninsula and parts of China. It is an anti-inflammatory plant and is used in contradiction of rheumatic and menstruation problems, as well as herniated discs and hyperosteogeny [13, 14]. There are many phenolic compounds in Cibotium that are potent antioxidants and strong chelators.…”

Gastroprotective Effect of Hydro-Alcoholic Extract of Polygonum bistorta Lin Root in Indomethacin- Induced Gastric Ulcers in Sprague Dawley Rats

“…Consequently, oxidative stress may cause induction and the aggravation of gastric ulcer. 20 Catalase and SOD are essential to the body in order to discharge the harmful ROS from the cellular environment. 21 The present study showed that mean SOD and catalase levels were significantly increased in extract treated-500 group-III, extract treated-1000 group-IV and standard drug treated group-V as compared to that decreased in ulcer control group-II rats.…”

Development and In Vitro Evaluation of a Zerumbone Loaded Nanosuspension Drug Delivery System

“…Similarly, Sidahmed et al showed antibacterial activity on S. choleraesuis, however no antifungal activity was observed, Moreover, it exerted an antiproliferative effect on HeLa cells [5]. Similarly, Sidahmed et al evaluated the gastroprotective effect of zerumbone and the results depict that zerumbone promotes ulcer protection along with antioxidant activity and maintenance of mucus integrity [6]. It also has been shown to have anti-inflammatory and antioxidant properties [7].…”