Antiscorpion Venom Activity of an Aromatic Compound having Carbohydrate Moiety Isolated from Hemidesmus indicus (Anantamul) Root Extract in Experimental Animal Models

Abstract

The present study established the anti-scorpion venom activity of an aromatic compound purified from the Indian medicinal plant Hemidesmus indicus (anantamul) root extract in experimental animals. The compound (H.I-1) was isolated by silica gel column chromatography and thin layer chromatography and spectral analysis was done by IR and H1 NMR. Animal ethical clearance was obtained before experiment. Anti-scorpion venom activity was evaluated using various in vivo tests, urine qualitative analysis, renal toxicity markers (urea and creatinine), hepatotoxicity markers (AST and ALT), myotoxic markers (LDH and CPK) and oxidative stress markers (LPO, GPX and GSH) in animal models. Statistical analysis was done by one way ANOVA, values expressed as mean ± SEM, P<0.05 was considered as statistically significant. Silica gel column chromatography I & II produced an active compound (H.I-1) which gave a single spot on TLC. IR and H1 NMR studies revealed that H.I-1 was aromatic compound having sugar (rhamnose) moiety. It could neutralize scorpion (Heterometrus bengalensis) venom induced hepatotoxicity, renal toxicity, myotoxicity and oxidative stress in experimental animals. These observations confirmed that the aromatic compound H.I-1, isolated from Hemidesmus indicus root extract might be useful in scorpion envenomation and warrants further detailed studies.

Box–Behnken design avenue for development and validation of high‐performance thin‐layer chromatography method for estimation of rutin in Hemidesmus indicus

“…The roots are used to treat leprosy, leukoderma, itching, skin disease, asthma, bronchitis, leukorrhea, dysentery, piles, syphilis, paralysis, urinary disorders, and diabetes (Manosi et al, 2017; Pansare et al, 2018). This has been experimentally proven to have antioxidant, antacid, antiarthritic, free radical scavenging, anticataract, antibacterial, anhihyperglycemic, antinociceptive, antiscorpion, antihyperlipidemic, in vitro acetylcholinesterase inhibitory, cardioprotective, antimicrobial, and wound healing and antiulcerogenic properties (Abiraamasri & Lakshmi, 2016; Farooque & Atar, 2016; Gomes et al, 2016; Joshi et al, 2018; Mehmood et al, 2016; Mohan et al, 2015; Saritha et al, 2015; Tirumani & Av, 2017). Mishra et al (2018) discussed the preliminary phytochemical screening and in vivo safety evaluation of an ethanolic extract of H. indicus (Linn.)…”