Antiradical and Oxidative Stress Release Properties of Trifolium pratense L. extract

Abstract

Low adaptive capacity and oxidative stress are the factors leading to cellular dysfunction, protein and lipid peroxidation, and the development of diseases. In recent decades, there has been a trend toward the active use of plant-based antioxidants. Trifolium pratense L. is a promising plant for the pharmaceutical and food industry and has anti-radical properties. This work is devoted to studying the antiradical and oxidative stress-released properties of T. pratense in Caenorhabditis elegans under oxidative and temperature stress. The objective of this research was to evaluate the anti-radical properties of the T. pratense extracts and individual BAS (chlorogenic acid, ononin, biochanin A, genistein) and analysis their influences on the oxidative stress of Caenorhabditis elegans in the presence of paraquat. Analysis of the antiradical properties revealed that chlorogenic acid has the maximum ability to neutralize the free radical (35.49µmol). A separate analysis of oxidative stress revealed high ononin activity at concentrations of 10, 50, and 100 µmol at 48 hours of cultivation. Biochanin A increases survival by 13.1% compared to the control. The use of the extract (500µmol) contributed to an increase in survival on day 1 of incubation. Under conditions of thermal stress, ononin (50 and 200 µmol) has a positive effect on the viability of C. elegans. The extract and BAS of T. pratense are characterized by high antiradical activity. In addition, the ability to influence the viability of C. elegans was revealed. Therefore, it is worthwhile to further study the biological properties of T. pratense for use in geroprotective therapy.