Antiradical and antidiabetic properties of standardized extract of Sunderban mangrove Rhizophora mucronata

Abstract

Background:Mangroves have the ability to grow where no other vascular plants survive. Rhizophora mucronata is a true mangrove and traditionally used to treat diabetes and its allied complications.Objectives:In the present study, we standardized the 80% methanolic standardized extract of R. mucronata leaves (RH) and found out its antiradical and antidiabetic activities.Materials and Methods:The methanolic extract of R. mucronata leaves (RH) was standardized and quantified for phenolics, flavonoinds, gallic acid, quercetin, and coumarin. The reducing abilities and antiradical activities of RH were performed in vitro methods like, 1,1-diphenyl-2-picrylhydrazyl, nitric oxides, superoxides, hydroxyl, and ABTS (2,2’-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid). Thereafter, RH was evaluated for it antidiabetic potentialities on streptozotocin (STZ)-induced type-2 diabetes. STZ (90 mg/kg, intraperitoneal) was administered to 2 days old pups to induce diabetes. RH was fed at doses of 50 and 100 mg/kg and glibenclamide (positive control) at 5 mg/kg, when the rats were 6 weeks old and continued for 10 weeks. Fasting glucose was monitored before and after the treatment. Further, lipid peroxides and reduced glutathione level were estimated on rat liver.Results:The results obtained from this study revealed RH possesses flavonoinds and also gallic acid, quercetin, and coumarin. Further, it has antiradical activities. It has also reduced blood glucose level in type-2 diabetic rats and reduced the formation of lipid peroxidation in liver. RH enhanced the level of glutathione in liver tissue.Conclusion:RH exhibits source of natural antioxidants and great potentialities as an antidiabetic agent by improving the hyperglycemia through its antiradical action.

Antidiabetic potential of mangrove plants: a review

“…Among these compounds, inositol and pinitol showed promising activity in the STZ model at 100 mg/kg dose level (Lakshmi et al 2006). The ethanolic leaf extracts of R. apiculata showed hypoglycaemic and antihyperglycaemic effect in normal, glucose-fed and STZ diabetic rats (Sur et al 2004(Sur et al , 2015. The hypoglycaemic action of R. apiculata is attributed to the presence of flavonoids along with other bioactive compounds.…”

Comparative evaluation of anti-diabetic activity of fresh juice and ethanolic extract of Sunderban mangrove Rhizophora mucronata Lam. leaves in animal model

“…19 Some recent studies also supported that hydro-alcoholic extract of R. mucronata leaves exhibited antiradical and anti-diabetic effects. 12,13 A study from Bangladesh demonstrated that, the ethanol extracts of Rhizophora mucronata Poir leaves showed significant inhibition of carbohydrate digestion and absorption, which has resulted in hypoglycemic effects of Rhizophora mucronata.…”

“…11 Recent study reported the anti-diabetic action of hydro-methanolic and hydro-ethanolic extract of R mucronata (Sundarban Mangrove) leaves in diabetes model. 12,13 Hence, the present study has undertaken to explore the comparative analysis between fresh juice and ethanolic extract of the leaves of Rhizophora mucronata Lam. in diabetic rat model.…”

Toxicity and Free Radical Scavenging Activity of Rhizophora Mucronata Lamk. Bark Extracts From Kendari Bay of Southeast Sulawesi Province Indonesia

“…The extracts of some mangrove plants show an antibacterial activity of Mycobacterium tuberculosis that has been resistant to a variety of previously administered drugs 12 . The test results performed by 13,14 showed the R. mucronata leaf extract had antihyperglycemic and antioxidant activity. The ethanol extracts of bark of R. mucronata Lamk.…”