Antipyrine estimations in the rabbit using gas-liquid chromatography: A reliable method for studying factors affecting oxidative drug metabolism

Effects of Drug Pretreatment on Antipyrine Elimination in the Rabbit

“…The dose of antipyrine was 50 mg/kg i.v. and blood samples were taken to be ana lysed by the method of Chambers and Jefferson [1981]. Rabbits received pretreatment with the drug vehicle prior to the first antipyrine estimation (con trol) followed by drug pretreatment prior to the second antipyrine estimation.…”

Absence of Effect of Propoxyphene on Antipyrine Kinetics in the Rabbit

“…The mean error (4.3%) for the slopes of the 14 regres sion lines from which the half-lives were cal culated. was less than that (6.3%) obtained by Chambers and Jefferson [6], who used seven blood samples taken over a 2.5-hour period to define antipyrine elimination. These results suggest that in the New Zealand white rabbit, propoxyphene does not inhibit the total plasma clearance of antipyrine.…”

“…Recently Chambers and Jefferson [6,7] have suggested that the antipyrine test carried out in rabbits is of value in studies of agents capable of altering oxidative drug metabolism. Thus the effect of propoxyphene on the elimination kinetics of antipyrine was examined in rabbits.…”

Antipyrine elimination in saliva after low‐dose combined or progestogen‐ only oral contraceptive steroids.

“…method of Chambers & Jefferson (1981) required 1 ml saliva samples and used phenoxybenzamine hydrochloride as the internal reference standard. The column was 5% OV 17 on 100-120 mesh Gas Chrom Q at a temperature of 260°C while the temperature of both the injection port and flame ionisation detector was 2750C.…”