Antiproliferative Triterpenoid Saponins from Leptaulus citroides Baill. from the Madagascar Rain Forest

Su, Qingxi; Brodie, Peggy J.; Liu, Yixi; Miller, James S.; Andrianjafy, Naina M.; Antsiferana, Rabodo; Rasamison, Vincent E.; Kingston, David G. I.

doi:10.1007/s13659-015-0083-1

Cited by 8 publications

(4 citation statements)

References 20 publications

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“…Due to the unsustainable harvesting of the bark and roots along with the fact that the natural propagation of WU is problematic, its availability has been declining throughout its nat- Up until now, there have been about 90 compounds isolated from WU, which are mainly composed of sesquiterpenes, flavonoids, fatty acids, lignanamides, and essential oils [25][26][27][28][29]. Some of the compounds isolated from other medicinal plants exhibiting anticancer activity have also been found in WU, such as cinnamosmolide, 6β-acetoxyisodrimenin, ungandensolide, futronolide, warburganal, polygodial, mukaadial, drimenol, and cinnamodial [30][31][32][33][34][35][36].…”

Section: Effect Of Wu Extracts On the Growth Of Human Cancer Cell Linesmentioning

confidence: 99%

Stimulation of ROS Generation by Extract of Warburgia ugandensis Leading to G0/G1 Cell Cycle Arrest and Antiproliferation in A549 Cells

et al. 2021

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Warburgia ugandensis Sprague (WU) is a traditional medicinal plant used for the treatment of various diseases, including cancer, in Africa. This study aimed to evaluate the anti-non-small cell lung cancer (NSCLC) activities of WU against A549 cells and to reveal potential molecular mechanisms. The cytotoxicity of various WU extracts was evaluated with HeLa (cervical cancer), HepG2 (liver cancer), HT-29 (colorectal cancer), and A549 (non-small cell lung cancer) cells by means of Sulforhodamine B (SRB) assay. Therein, the dimethyl carbonate extract of WU (WUD) was tested with the most potent anti-proliferative activity against the four cancer cell lines, and its effects on cell viability, cell cycle progression, DNA damage, intracellular reactive oxygen species (ROS), and expression levels of G0/G1-related proteins in A549 cells were further examined. First, it was found that WUD inhibited the proliferation of A549 cells in a time- and dose-dependent manner. In addition, WUD induced G0/G1 phase arrest and modulated the expression of G0/G1 phase-associated proteins Cyclin D1, Cyclin E1, and P27 in A549 cells. Furthermore, WUD increased the protein abundance of P27 by inhibiting FOXO3A/SKP2 axis-mediated protein degradation and also significantly induced the γH2AX expression and intracellular ROS generation of A549 cells. It was also found that the inhibitory effect of WUD on the proliferation and G0/G1 cell cycle progression of A549 cells could be attenuated by NAC, a ROS scavenger. On the other hand, phytochemical analysis of WUD with UPLC-QTOF-MS/MS indicated 10 sesquiterpenoid compounds. In conclusion, WUD exhibited remarkable anti-proliferative effects on A549 cells by improving the intracellular ROS level and by subsequently modulating the cell proliferation and G0/G1 cell cycle progression of A549 cells. These findings proved the good therapeutic potential of WU for the treatment of NSCLC.

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Section: Effect Of Wu Extracts On the Growth Of Human Cancer Cell Linesmentioning

confidence: 99%

Stimulation of ROS Generation by Extract of Warburgia ugandensis Leading to G0/G1 Cell Cycle Arrest and Antiproliferation in A549 Cells

et al. 2021

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“…All three new compounds were tested for their toxicity against brine shrimp, and the results indicated that all exhibited moderate activity, with LD 50 values of 0.9, 2.2, and 2.1 µM, respectively. 8) Bioassay-guided fractionation of the ethyl acetate (EtOAc) fraction of the stem bark of Muntafara sessilifolia (Apocynaceae) led to the isolation of four vobasinyl-iboga bisindoles (25)(26)(27)(28) and one 2-acyl monomeric indole (29) alkaloid, along with eight known alkaloids (3-oxocoronaridin hydroxyindolenine, eglandulosine, eglandine, coronaridine, tabernaemontanine, and tabernaelegantines A, B, and D). All the isolated compounds were evaluated for their ability to inhibit the in vitro intraerythrocytic development of the chloroquine-resistant strain FcB1 of P. falciparum.…”

Section: Alkaloidsmentioning

confidence: 99%

“…Although 66 displayed interesting activity, its low therapeutic index (0.788) was discouraging. 26) Bioassay-guided fractionation of EtOH extracts obtained from the roots and wood of the Madagascan plant Leptaulus citroides BAILL. (Cardiopteridaceae) resulted in the isolation of ethyl esters of three new triterpenoid saponins (67-69), together with the known compound cinnamosmolide.…”

Section: Alkaloidsmentioning

confidence: 99%

Novel Bioactive Natural Products Isolated from Madagascar Plants and Marine Organisms (2009–2017)

Dai

Liu

Rakotondraibe

2018

CHEMICAL & PHARMACEUTICAL BULLETIN

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Madagascar's rain forests and tropical dry forests are home to numerous endemic plant species and the island is considered a biodiversity hotspot. About 80% of the Madagascan (Malagasy) population relies on traditional medicines that have been proven to contain a variety of biologically active compounds. In the search for bioactive compounds from Madagascan biodiversity, we accessed and collected most of the literature dealing with the isolation, structure elucidation, and biological activities of organic small molecules originating from Madagascan plants and marine organisms. Since we published the first review of this work in 2009 (Curr. Med. Chem., 17, 2010, Hou and Harinantenaina), the present paper covers the isolation, structures, and bioactivity of 182 new secondary metabolites isolated from Malagasy higher plants and marine organisms in the last seven years (2009-2017).

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“…According to an ethnobotanical study, W. ugandensis was used to treat breast cancer, cervical cancer, prostate cancer, skin cancer, throat cancer and intestinal cancer in the rural Greater Mpigi region of Uganda [10]. In addition, a number of studies have documented the cytotoxicity of some compounds in W. ugandensis or in other plant extracts against different types of cancer, including mouth epidermoid carcinoma [5,11,12], ovarian cancer [13], breast cancer [14,15], prostate cancer [16], melanoma [14,17] and glioblastoma [14]. Our previous study showed that WUD exhibited potent inhibitory growth activity against HT-29 CRC cells.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Growth of Colon Tumors and Proliferation of HT‐29 Cells by Warburgia ugandensis Extract through Mediating G₀/G₁ Cell Cycle Arrest, Cell Apoptosis, and Intracellular ROS Generation

Zhang

Chen

Zhuang

2021

Oxidative Medicine and Cellular Longevity

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Warburgia ugandensis Sprague (W. ugandensis), widely distributed in Africa, is a traditional medicinal plant used for the treatment of various diseases including cancer. We intended to evaluate the anticolorectal cancer (CRC) activities of the crude extract from W. ugandensis (WUD) and reveal the underlying molecular mechanisms of its action. We found that WUD inhibited the proliferation of HT-29 and HCT116 cells in a time- and dose-dependent manner and induced intracellular ROS generation. The inhibitory effect of WUD on the proliferation of HT-29 and HCT116 cells could be attenuated by NAC (a ROS scavenger) in a dose-dependent manner. WUD induced G0/G1 phase arrest, down-regulated the protein expression of Cyclin D1 via ROS accumulation in HT-29 cells. In search of the molecular mechanism involved in WUD-induced Cyclin D1 down-regulation, it was found that WUD can suppress PI3K/Akt/GSK3β signaling pathway in HT-29 cells. Next, it was found that WUD also activated apoptosis, poly-ADP ribose polymerase 1 (PARP1) cleavage and down-regulated pro-caspase 3 in HT-29 and HCT116 cells. Besides, WUD decreased the growth of colon tumors in vivo in the xenograft mouse model. We demonstrated for the first time that ROS and their modulation in the corresponding intracellular signaling could play a significant role in the potential activity of WUD against CRC cells.

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Antiproliferative Triterpenoid Saponins from Leptaulus citroides Baill. from the Madagascar Rain Forest

Cited by 8 publications

References 20 publications

Stimulation of ROS Generation by Extract of Warburgia ugandensis Leading to G0/G1 Cell Cycle Arrest and Antiproliferation in A549 Cells

Stimulation of ROS Generation by Extract of Warburgia ugandensis Leading to G0/G1 Cell Cycle Arrest and Antiproliferation in A549 Cells

Novel Bioactive Natural Products Isolated from Madagascar Plants and Marine Organisms (2009–2017)

Inhibition of Growth of Colon Tumors and Proliferation of HT‐29 Cells by Warburgia ugandensis Extract through Mediating G₀/G₁ Cell Cycle Arrest, Cell Apoptosis, and Intracellular ROS Generation

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Antiproliferative Triterpenoid Saponins from Leptaulus citroides Baill. from the Madagascar Rain Forest

Cited by 8 publications

References 20 publications

Stimulation of ROS Generation by Extract of Warburgia ugandensis Leading to G0/G1 Cell Cycle Arrest and Antiproliferation in A549 Cells

Stimulation of ROS Generation by Extract of Warburgia ugandensis Leading to G0/G1 Cell Cycle Arrest and Antiproliferation in A549 Cells

Novel Bioactive Natural Products Isolated from Madagascar Plants and Marine Organisms (2009–2017)

Inhibition of Growth of Colon Tumors and Proliferation of HT‐29 Cells by Warburgia ugandensis Extract through Mediating G0/G1 Cell Cycle Arrest, Cell Apoptosis, and Intracellular ROS Generation

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Inhibition of Growth of Colon Tumors and Proliferation of HT‐29 Cells by Warburgia ugandensis Extract through Mediating G₀/G₁ Cell Cycle Arrest, Cell Apoptosis, and Intracellular ROS Generation