Antiproliferative effect of upregulation of hsa-let-7c-5p in human acute erythroleukemia cells

Mortazavi, Deniz; Sharifi, Mohammadreza

doi:10.1007/s10616-018-0241-5

Cited by 16 publications

(9 citation statements)

References 46 publications

(59 reference statements)

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“…26 However, hsa-let-7a-5p, hsa-let -7b-5p and hsa-let-7c-5p play an important role in the occurrence and development of colorectal cancer, multiple myeloma and acute lymphoblastic leukemia. [27][28][29] This finding provides a theoretical basis for understanding the working mechanism of hsa_circRNA_012515 in the occurrence and development of NSCLC.…”

Section: Discussionmentioning

confidence: 76%

<p>hsa_circRNA_012515 Is Highly Expressed in NSCLC Patients and Affects Its Prognosis</p>

Fu¹,

Huang²,

Tang³

et al. 2020

CMAR

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Background: NSCLC is one of the most common and most lethal malignancies throughout the world, and there is still a lack of sensitive diagnostic biomarkers. Identifying novel NSCLC biomarkers can help with the diagnosis and clinical decision-making. Methods: Identifying the differentially expressed circRNAs in three cases of NSCLC tissues by high-throughput circRNA microarray sequencing. qRT-PCR was employed to detect the expression levels of hsa_circRNA_012515 in 80 cases of NSCLC tissues (tumor resection patients) and 60 cases of peripheral blood samples (chemotherapy patients), NSCLC cells and gefitinibresistant NSCLC cell lines. Then combining with clinical data, we discussed whether it was feasible to use hsa_circRNA_012515 as the diagnostic and prognostic biomarker for NSCLC. Results: In the cancerous tissues from NSCLC patients, NSCLC cells and gefitinib-resistant cell lines, the average expressions of hsa_circRNA_012515 increased significantly (P<0.01). Patients of stage III-IV, with lymph node metastases, had an overexpression of hsa_circRNA_012515. High expression of hsa_circRNA_012515 was associated with lower OS and shorter PFS, and it is closely related to the prognosis of the patients. Bioinformatic analysis indicated that hsa_circRNA_012515 interacted with 5 miRNAs. This finding may shed new light on the subsequent studies on the working mechanism and functions. Conclusion: Our study showed that hsa_circRNA_012515 may be a novel biomarker candidate for NSCLC. However, further studies are needed to ascertain the working mechanism of hsa_circRNA_012515 in the occurrence and development of NSCLC.

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Section: Discussionmentioning

confidence: 76%

<p>hsa_circRNA_012515 Is Highly Expressed in NSCLC Patients and Affects Its Prognosis</p>

Fu¹,

Huang²,

Tang³

et al. 2020

CMAR

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“…In Axis 1, 4, 5, 6 ( Table 2 ) , we observed abnormal expression of various circRNA-miRNA pairs, some of which were previously found to be associated with cancer. In Axis 1 ( Table 2 ) , has_circ_0027494↑ let-7c-5p↓, let-7c-5p is found to be a tumor suppressor across many cancer types, including breast cancer ( Fu et al, 2017 ), mucosal melanoma ( Tang et al, 2019 ), acute erythroleukemia ( Mortazavi and Sharifi, 2018 ), among others ( Wagner et al, 2014 ; Liu et al, 2018 ; Chirshev et al, 2019 ). We postulate that hsa_circ_0027494 has inhibitory effects on let-7c-5p ( Panda, 2018 ; Zhang P.-F. et al, 2019 ; Zhang X. et al, 2019 ), leading to downstream overexpression of let-7c-5p targeted genes, which could explain tumorigenic activities that stands differently in EBV + vs EBV- NPC.…”

Section: Discussionmentioning

confidence: 99%

Competitive Endogenous RNA Landscape in Epstein-Barr Virus Associated Nasopharyngeal Carcinoma

Lin

Wang

Kang

et al. 2021

Front. Cell Dev. Biol.

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Non-coding RNAs have been shown to play important regulatory roles, notably in cancer development. In this study, we investigated the role of microRNAs and circular RNAs in Nasopharyngeal Carcinoma (NPC) by constructing a circRNA-miRNA-mRNA co-expression network and performing differential expression analysis on mRNAs, miRNAs, and circRNAs. Specifically, the Epstein-Barr virus (EBV) infection has been found to be an important risk factor for NPC, and potential pathological differences may exist for EBV+ and EBV- subtypes of NPC. By comparing the expression profile of non-cancerous immortalized nasopharyngeal epithelial cell line and NPC cell lines, we identified differentially expressed coding and non-coding RNAs across three groups of comparison: cancer vs. non-cancer, EBV+ vs. EBV- NPC, and metastatic vs. non-metastatic NPC. We constructed a ceRNA network composed of mRNAs, miRNAs, and circRNAs, leveraging co-expression and miRNA target prediction tools. Within the network, we identified the regulatory ceRNAs of CDKN1B, ZNF302, ZNF268, and RPGR. These differentially expressed axis, along with other miRNA-circRNA pairs we identified through our analysis, helps elucidate the genetic and epigenetic changes central to NPC progression, and the differences between EBV+ and EBV- NPC.

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“…In parallel, it was noted that the expression of hsa-let-7c-5p was associated with the level of transcriptional activity of the BEGAIN gene; the link between hsa-miR-125b-5p and Here, the relation between the expression of the listed genes and overall survival according to the results of our study is indicated the mRNA level of the DUSP3 and RAB3D genes was similarly found. Earlier, in an experimental study on cells of acute erythroleukaemia, the antiproliferative effect of let-7c-5p was demonstrated to be associated with the targeting of the PBX2 oncogene [24]. In colorectal cancer cells after exposure to 5-FU or starvation, Hou N et al identified a miRNA pool including hsa-let-7c-5p [25].…”

Section: Discussionmentioning

confidence: 99%

Integrative analysis of mRNA and miRNA sequencing data for gliomas of various grades

Gvaldin

Pushkin

Тимошкина

et al. 2020

Egypt J Med Hum Genet

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Background The purpose of this study was to characterize subtype-specific patterns of mRNA and miRNA expression of gliomas using The Cancer Genome Atlas (TCGA) data to search for genetic determinants that predict prognosis in terms of overall survival and to create interaction networks for grade 2 and 3 (G2 and G3) astrocytomas, oligodendrogliomas and grade 4 (G4) glioblastoma multiforme. Based on open-access TCGA data, 5 groups were formed: astrocytoma G2 (n = 58), astrocytoma G3 (n = 128), oligodendroglioma G2 (n = 102), oligodendroglioma G3 (n = 72) and glioblastoma G4 (n = 564); normal samples of brain tissue were also analysed (n = 15). Data of patient age, sex, survival and expression patterns of mRNA and miRNA were extracted for each sample. After stratification of the data into groups, a differential analysis of expression was carried out, genes and miRNAs that affect overall survival were identified and gene set enrichment analysis (GSEA) and interaction analysis were performed. Results A total of 939 samples of glial tumours were analysed, for which subtype-specific expression profiles of genes and miRNAs were identified and networks of mRNA-miRNA interactions were constructed. Genes whose aberrant expression level was associated with survival were determined, and pairwise correlations between differential gene expression (DEG) and differential miRNA expression (DE miRNA) were calculated. Conclusions The developed panel of genes and miRNAs allowed us to differentiate glioma subtypes and evaluate prognosis in terms of the overall survival of patients. The regulatory miRNA-mRNA pairs unique to the five glioma subtypes identified in this study can stimulate the development of new therapeutic approaches based on subtype-specific mechanisms of oncogenesis.

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Antiproliferative effect of upregulation of hsa-let-7c-5p in human acute erythroleukemia cells

Cited by 16 publications

References 46 publications

<p>hsa_circRNA_012515 Is Highly Expressed in NSCLC Patients and Affects Its Prognosis</p>

<p>hsa_circRNA_012515 Is Highly Expressed in NSCLC Patients and Affects Its Prognosis</p>

Competitive Endogenous RNA Landscape in Epstein-Barr Virus Associated Nasopharyngeal Carcinoma

Integrative analysis of mRNA and miRNA sequencing data for gliomas of various grades

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