Antiproliferative effect of the tree and medicinal species <i>Luehea divaricata</i> on the <i>Allium Cepa</i> cell cycle

Frescura, Viviane Dal-Souto; Laughinghouse, Haywood Dail; Tedesco, Solange Bósio

doi:10.1080/00087114.2012.678083

Cited by 29 publications

(24 citation statements)

References 9 publications

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“…Arg.) Briton and Psychotria birotula by Smith & Downs also showed no antimutagenic activity (Frescura, 2012).Relatively to the positive control (glyphosate), the recoveries in water and in the extract with a greater concentration presented no significant variation (χ 2 =6.080 and χ 2 =4.646, respectively), where the same was observed for recovery at 5 g.L -1 (χ 2 =0.013). With regard to the number of chromosomal alterations of these treatments in relation to the positive control, the results observed were not significant.…”

supporting

confidence: 53%

“…Likewise, Frescura et al (2012) using Luehea divaricata Martius extracts, also found that the extracts studied showed no genotoxic potential; however, antiproliferative capacity was evident, akin to the pomegranate extracts studied.…”

Section: Allium Cepa Testmentioning

confidence: 99%

“…Arg.) Briton and Psychotria birotula by Smith & Downs also showed no antimutagenic activity (Frescura, 2012).…”

Section: Allium Cepa Testmentioning

confidence: 99%

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Chromatographic analysis and antiproliferative potential of aqueous extracts of Punica granatum fruit peels using the Allium cepa test

Kuhn

Tedesco

Boligon

et al. 2015

Braz. J. Pharm. Sci.

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Punica granatum L., locally known as romanzeira, is native to Asia but found throughout Brazil. P. granatum is used for treating inflammatory, infectious and respiratory diseases. The aim of this study was to evaluate the chromatography and genotoxicity of an aqueous extract of P. granatum (pomegranate) fruit peel using the Allium cepa L. test. The experiment set-up entailed 7 treatments: T1-distilled water, T2-tea 5 g.L -1 , T3-tea 10 g.L -1 , T4-glyphosate at 9.6%, T5-glyphosate with subsequent recovery in distilled water, T6-glyphosate with subsequent recovery in tea 5 g.L -1 and T7-glyphosate with subsequent recovery in tea 10 g.L -1 . The rootlets were collected and fixed in ethanol:acetic acid (3:1) for 24 hours, then stored in 70% ethanol under refrigeration. Analysis was performed using high performance liquid chromatography for the quantification of the extracted phenolic compounds. Gallic acid, catechin, caffeic acid, and rutin were abundant in the extracts of P. granatum. The extracts were found to exhibit antiproliferative potential but not antimutagenic or genotoxic activity.Uniterms: Punica granatum L./phytochemistry. Punica granatum L./genotoxicity Punica granatum L./ chromatography analysis. Medicinal plants. Pomegranate.Punica granatum L., conhecida como romanzeira, é originária da Ásia e encontra-se distribuída por todo Brasil. É usada para o tratamento de doenças inflamatórias, infecciosas e respiratórias. Em decorrência da grande utilização de recursos fitoterápicos, é necessário esclarecer à população sobre a grande quantidade de substâncias existentes nas plantas e sobre os benefícios e prejuízos de tais substâncias à saúde. O presente trabalho objetivou realizar a análise cromatográfica e o estudo da genotoxicidade dos extratos aquosos das cascas dos frutos de P. granatum através do teste de Allium cepa L. Para a montagem do experimento, foram utilizados 7 tratamentos: T1-água destilada, T2-chá 5 g.L -1 , T3-chá 10 g.L -1 , T4-glifosato a 9,6%, T5-glifosato para recuperação em água destilada, T6-glifosato para recuperação em chá 5 g.L -1 e T7-glifosato para recuperação em chá 10 g.L -1 . As radículas foram coletadas e fixadas em etanol:ácido acético (3:1) por 24 horas, e armazenadas em álcool 70%, sob refrigeração. Realizouse análise por cromatografia líquida de alta eficiência para quantificação dos compostos fenólicos. Nos extratos de P. granatum foram observados em maior quantidade: ácido gálico, catequina, ácido cafeico e rutina. Além disso, os extratos demonstraram potencial antiproliferativo, sem apresentar atividade antimutagênica e genotóxica. (Braga, 1961) and is cultivated around the world in regions with tropical and subtropical climates. It is used as an ornamental plant, as a fruit, and for its medicinal properties (Corrêa, 1978). UnitermosAccording to Garcia (1992), the species has antiinflammatory and antibacterial properties. Naqyi et al. (1991) evaluated the antimicrobial activity of an extract of P. granatum on gram-positive bacteria and yeast, finding antimicrobial ac...

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confidence: 53%

Section: Allium Cepa Testmentioning

confidence: 99%

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Chromatographic analysis and antiproliferative potential of aqueous extracts of Punica granatum fruit peels using the Allium cepa test

Kuhn

Tedesco

Boligon

et al. 2015

Braz. J. Pharm. Sci.

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“…Onion (Allium cepa L.) root meristem cells are very sensitive to genetic damage by chemicals, and the Allium test, involving the length of the root and chromosome aberration (CA) measurements, proved to be a suitable model system for measuring the environmental cytogenetic potential of pollutants (Al-Sabti 1989). The Allium test is very good indicator for analysing antiproliferative effects on plant medicinal extracts (Frescula et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Chromosome damage studies in the onion plantAllium cepaL.

Firbas¹,

Amon²

2014

Caryologia

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The onion plant (Allium cepa L.) is a suitable indicator plant for the determination of potential genotoxic agents in the samples taken from the environment. The genotoxic level of the agent under study is reflected by structural changes of the chromosomes and their changed numbers. The chromosomes under study are taken from the meristem cells of the young growing onion roots. A healthy normal onion cell has 16 (2n = 16) chromosomes. They are relatively large and so very appropriate for the detection of morphological changes. Prior to the chromosome study the root tip cells were immersed in a 0.1% aquatic solution of colchicine which stopped the mitotic cycle continuing beyond metaphase. The changes in morphology varied from a single distortion of a single chromosome up to several morphological changes observed on many chromosomes. We identified 15 categories of morphological aberrations which are classified into three groups: chromatid damage (CtD), centromere damage (CmD) and chromosome damage (CsD). CtD includes: single break chromatid, double break chromatid, isochromatid break, multiple break chromatid, gap chromatid, centric ring chromatid, acentric ring chromatid and triradial chromosomes. CmD includes: break centromere, gap centromere, single break centromere, double break centromere and multiple break centromere. CsD includes: ring chromosomes and dicentric chromosomes. Sometimes also the chromosome number changed which occurred as aneuploidy with monosomy 2n = 15 (2n =16-1) and euploidyincreased number of the basic chromosome number (2n = 6x-8x). We identified also the translocation: t(3p-; 5p+).

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“…Allium cepa L. has been used to evaluate DNA damage, such as chromosome aberrations, micronuclei and disturbances in the mitotic cycle (Bakare et al 2012;Frescura et al 2012;Olorunfemi et al 2012;Achary et al 2013). Due to its sensitivity, the A. cepa test was the first of nine plants assay systems evaluated by the Gene-Tox Program of the US Environmental Protection Agency (Grant 1994).…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic effects of chitosan-capped silver nanoparticles in theAllium cepatest

Pesnya

2013

Caryologia

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Silver nanoparticles are one of the most commonly used nanomaterials. However, there remains insufficient information on their genotoxic effects. The goal of this study was to investigate cytotoxic and genotoxic effects of chitosan-coated AgNPs using the well-established Allium test. Root tip cells were treated with solutions at different concentrations (1, 2.5, 5 and 50 mg l . The absence of induction of chromosomal and mitotic abnormalities by chitosan-capped AgNPs at low concentrations is possibly due to the capping, which may partly protect the cells from direct interaction with the AgNPs. Mitotic and chromosomal abnormalities and micronuclei were detected at a concentration of 50 mg l −1 . Significant increase in mitotic index was found at 5 and 50 mg l −1 concentrations of AgNP. The data demonstrated that chitosan-coated AgNP exhibit both clastogenic and aneugenic activity.

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Antiproliferative effect of the tree and medicinal species Luehea divaricata on the Allium Cepa cell cycle

Cited by 29 publications

References 9 publications

Chromatographic analysis and antiproliferative potential of aqueous extracts of Punica granatum fruit peels using the Allium cepa test

Chromatographic analysis and antiproliferative potential of aqueous extracts of Punica granatum fruit peels using the Allium cepa test

Chromosome damage studies in the onion plantAllium cepaL.

Cytogenetic effects of chitosan-capped silver nanoparticles in theAllium cepatest

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