Antiproliferative and Cytotoxic Evaluation of Herbal Supplement SAABFAT6 on HT29 Colorectal Adenocarcinoma Cells

Abstract

The consumption of herbal supplements has been one of the remedy for several aliments including cancer for a long time. Cancers, especially colorectal adenocarcinoma are diseases with high morbidity and mortality and are often associated with suffering and poor quality of life. Herbal supplements are an attractive cancer therapy, we have investigated the antiproliferative and cytotoxic properties of the herbal supplement SAABFAT6 on colorectal adenocarcinoma cell (HT29). Ethanol extracts of SAABFAT6 (0.5‐2 mg/mL) was incubated with HT29 and in vitro antiproliferative/cytotoxicity activities were evaluated using MTT assay. Treatment of HT29 with SAABFAT6 significantly reduced cellular proliferation at 48 and 72 but not at 24 hrs except for 2 mg/mL concentration. Cytotoxic evaluation showed significant reduction in cell viability at 24 and 48 hrs (2 mg/mL) and at 48 hrs (0.5 and 1 mg/mL). This preliminary result indicates that SAABFAT6 supplement may have a potential for the regulation of HT29 growth and survival. Further study is required to identify the mechanism(s) by which the antiproliferative and cytotoxic effects of SAABFAT6 is mediated.