Antiproliferative and Apoptotic Activities of Natural Honey

Mumtaz, Peerzada Tajamul; Bashir, Showkeen Muzamil; Rather, Muzafar Ahmad; Dar, Khalid Bashir; Taban, Qamar; Sajood, Saima; Ali, Aarif; Rather, Zubair Ahmad; Amin, Insha; Dar, Muneer Ahmad

doi:10.1007/978-981-15-6799-5_18

Cited by 7 publications

(7 citation statements)

References 43 publications

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“…The phenomenon of cytotoxic effects of different types of honey has only recently been recognised and described in the literature ( 25 , 26 ). Recently, Imtara et al ( 27 ) reported cytotoxic effects of different honeys collected in Morocco and Palestine on human colon adenocarcinoma (HCT-116) and breast cancer (MCF-7) cell lines.…”

Section: Resultsmentioning

confidence: 99%

Cytotoxic activity of strawberry tree (Arbutus unedo L.) honey, its extract, and homogentisic acid on CAL 27, HepG2, and Caco-2 cell lines

Jurič

Turković

Karačonji

et al. 2022

Archives of Industrial Hygiene and Toxicology

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Strawberry tree (Arbutus unedo L.) honey (STH), also known as “bitter honey”, is a traditional medicine widely used in the Mediterranean area. Regardless of geographical origin, it usually has a very high content of phenolic compounds and strong antioxidant capacity. Yet, little is still known about the effects of STH, its phenolic extract (STHE), and its main bioactive compound – homogentisic acid (HGA) – at the cell level. The aim of this study was to estimate total phenolic content, DPPH radical scavenging activity, and ferric reducing antioxidant power of STH made in Croatia and investigate cytotoxic and pro-oxidative effects of STH, STHE and HGA on three human cell lines: tongue squamous cell carcinoma (CAL 27), hepatocellular carcinoma (HepG2), and epithelial colorectal adenocarcinoma cells (Caco-2) cells. These substances were tested at four concentrations (0.5–5× average human daily intake of STH) and over 30 min and 1 and 2 h. Croatian STH had a total phenolic content of 1.67 g gallic acid equivalents (GAE) per kg of honey, DPPH radical scavenging activity of 2.96 mmol Trolox equivalents (TE) per kg of honey, and ferric reducing antioxidant power (FRAP) of 13.5 mmol Fe2+ per kg of honey. Our results show no clear and consistent time- or concentration-dependent cytotoxicity in any of the cell lines. ROS levels in all the three cell types at almost all exposure times were not significantly higher than control. The most important observation is that the tested substances have low cytotoxicity and high biocompatibility, regardless of concentration, which is a good starting point for further research of their biological effects in other models.

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Section: Resultsmentioning

confidence: 99%

Cytotoxic activity of strawberry tree (Arbutus unedo L.) honey, its extract, and homogentisic acid on CAL 27, HepG2, and Caco-2 cell lines

Jurič

Turković

Karačonji

et al. 2022

Archives of Industrial Hygiene and Toxicology

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“…The existing literature reports the antiproliferative properties of various types of honey [8,[32][33][34][35], as well as antiproliferative and cytostatic effects caused after exposure to the same or related phenolics as those present in our tested sample. Almost all of the abovementioned papers that report on cytotoxic or apoptotic effects of specific phenolic compounds also point to their antiproliferative properties [36][37][38][39][40][41][42][43][44][45][46].…”

Section: Discussionmentioning

confidence: 99%

“…Based on the score of non-viable cells, it is evident that our tested sample of STH possessed low cytotoxic potency. The phenomenon of cytotoxic effects exerted by various types of honey has been well recognized and reviewed [32,33]. Recently, Imtara et al [34] observed the cytotoxic effects of some Moroccan and Palestinian honeys on human cell lines HCT-116 and MCF-7.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study

Jurič

Karačonji²,

Gašić³

et al. 2023

IJMS

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Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap–Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.

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“…On the other hand, studies in human leukemia cells K562 show that the action of quercetin is not only focused on the inhibition of cell proliferation but also is able to induce apoptosis at concentrations of 80 µM and also causes a downregulation of cellular myelocytomatosis (c-myc) and Kirsten RAt sarcoma (K-ras) oncogenes [ 78 ]. The proapoptotic effect of quercetin can be partly explained because the compound’s antioxidative effect changes entirely to a prooxidant effect at high concentrations, which induces selective cytotoxicity [ 79 ].…”

Section: Quercetin Particularitiesmentioning

confidence: 99%

Quercetin, a Flavonoid with Great Pharmacological Capacity

Carrillo-Martinez,

Flores-Hernández,

Salazar-Montes

et al. 2024

Molecules

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Quercetin is a flavonoid with a low molecular weight that belongs to the human diet’s phenolic phytochemicals and nonenergy constituents. Quercetin has a potent antioxidant capacity, being able to capture reactive oxygen species (ROS), reactive nitrogen species (RNS), and reactive chlorine species (ROC), which act as reducing agents by chelating transition-metal ions. Its structure has five functional hydroxyl groups, which work as electron donors and are responsible for capturing free radicals. In addition to its antioxidant capacity, different pharmacological properties of quercetin have been described, such as carcinostatic properties; antiviral, antihypertensive, and anti-inflammatory properties; the ability to protect low-density lipoprotein (LDL) oxidation, and the ability to inhibit angiogenesis; these are developed in this review.

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Antiproliferative and Apoptotic Activities of Natural Honey

Cited by 7 publications

References 43 publications

Cytotoxic activity of strawberry tree (Arbutus unedo L.) honey, its extract, and homogentisic acid on CAL 27, HepG2, and Caco-2 cell lines

Cytotoxic activity of strawberry tree (Arbutus unedo L.) honey, its extract, and homogentisic acid on CAL 27, HepG2, and Caco-2 cell lines

Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study

Quercetin, a Flavonoid with Great Pharmacological Capacity

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