Antiproliferative and apoptosis inducing effects of citral via p53 and ROS-induced mitochondrial-mediated apoptosis in human colorectal HCT116 and HT29 cell lines

Sheikh, Bassem Y.; Sarker, Md. Moklesur Rahman; Kamarudin, Muhamad Noor Alfarizal; Mohan, Gokula

doi:10.1016/j.biopha.2017.10.038

Cited by 59 publications

(35 citation statements)

References 66 publications

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“…HT-29 cells were pretreated for 24 h with the extracts at the established concentrations (IC 50 ). As shown in Figure 3b HT29 cell lines (Sheikh, Sarker, Kamarudin, & Mohan, 2017). Similar results were observed for p21 and p27, which are downstream proteins of p53.…”

Section: Cell Viabilitysupporting

confidence: 81%

“…In general, both cultivars present accumulation of these proteins; however, Negro 8025 generated higher accumulation than Bayo Victoria beans. Protein p53, involved in the induction of cell cycle arrest and/or apoptosis, was accumulated (4.1–3.3 fold), indicating that the upregulation leads to the inhibition of cancer cell proliferation in human colorectal HCT116 and HT29 cell lines (Sheikh, Sarker, Kamarudin, & Mohan, ). Similar results were observed for p21 and p27, which are downstream proteins of p53.…”

Section: Resultsmentioning

confidence: 99%

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Mechanisms associated to apoptosis of cancer cells by phenolic extracts from two canned common beans varieties (Phaseolus vulgarisL.)

et al. 2018

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Two varieties of common beans (Phaseolus vulgaris L.), Bayo Victoria and Negro 8025, were evaluated to determine the effect on cellular viability and mechanisms involved in apoptosis pathways, using a cellular model with HT‐29 cells. Aqueous methanolic (50:50) extracts from cooked beans were analyzed for phenolic composition, identifying greater diversity of phenolic compounds in Bayo Victoria extracts. However, Negro 8025 showed greater phenolic content and cytotoxicity effects at lower media inhibitory concentrations, and greater effectiveness to activate apoptotic pathways. Proteins related to the arrest of cell cycle were modulated by both bean cultivars. Qualitative analysis by HPLC‐PAD and HPLC‐MS systems of phenolic compounds in common bean extracts showed mainly hydroxybenzoic and hydroxycinnamic acids, flavonols, and monomeric flavan‐3‐ols. Bioactive phenolics such as catechin, kaempferol, and ferulic acid were found in both cultivars as well anticancer phytochemicals such as quercetin, protocatechuic acid, myricetin, naringenin and their derivatives, and procyanidins. Practical applications Polyphenols in common beans (Phaseolus vulgaris L.) cultivars processed by canning display chemoprotective potential as they activate mechanisms involved in apoptosis pathways. Phenolics in common beans modulate 28 proteins related to apoptotic processes. Therefore, a diet including canned beans (particularly darker varieties) might represent health benefits and cancer‐preventive effects.

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Section: Cell Viabilitysupporting

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Mechanisms associated to apoptosis of cancer cells by phenolic extracts from two canned common beans varieties (Phaseolus vulgarisL.)

et al. 2018

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“…Recent studies have shown citral to inhibit cell intracellular levels of oxygen radicals and, consequently, by the modulation of cellular oxidative status, leading to reduction of cancer cell proliferation and ultimately resulting in cell death (Kapur et al, 2016;Sanches et al, 2017). Citral was also shown to induce caspase activation and subsequent apoptosis induction in several cancer cell types, including colorectal cancer and glioblastoma (Dudai et al, 2005;Queiroz et al, 2014;Sheikh et al, 2017). Other studies have shown that citral is a is a key component of the essential oils extracted from lemon balm with antiproliferative effect in several leukemia cell lines (P388, NB4, MOLT-4) via the induction of apoptosis (Xia et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

In vitro antiproliferative activity of Melissa officinalis L (Lamiaceae) leaves essential oil

Nikšić¹,

Durić²,

Sijamic³

et al. 2019

Bol Latinoam Caribe Plant Med Aromat

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In the present study, we investigated the antiproliferative activity of essential oil from leaves of Melissa officinalis L. grown in Southern Bosnia and Herzegovina. In vitro evaluation of antiproliferative activity of the M. officinalis essential oil was carried out on three human tumor cell lines: MCF-7, NCI-H460 and MOLT-4 by MTT assay. M. officinalis essential oil was characterized by high percentage of monoterpenes (77,5%), followed by the sesquiterpene fraction (14,5%) and aliphatic compounds (2,2%). The main constituents of the essential oil of M. officinalis are citral (47,2%), caryophyllene oxide (10,2%), citronellal (5,4%), geraniol (6,6%), geranyl acetate (4,1%) and βcaryophyllene (3,8%). The essential oil showed significant antiproliferative activity against three cancer cell lines, MOLT-4, MCF-7, and NCI-H460 cells, with GI50 values of <5, 6±2 and 31±17 µg/mL, respectively. The results revealed that M. officinalis L. essential oil has a potential as anticancer therapeutic agent.Resumen: En el presente estudio, investigamos la actividad antiproliferativa del aceite esencial de las hojas de Melissa officinalis L. cultivadas en el sur de Bosnia y Herzegovina. La evaluación in vitro de la actividad antiproliferativa del aceite esencial de M. officinalis se llevó a cabo en tres líneas celulares de tumores humanos: MCF-7, NCI-H460 y MOLT-4 utilizando el ensayo de MTT. El aceite esencial de M. officinalis se caracterizó por un alto porcentaje de monoterpenos (77,5%), seguido de la fracción sesquiterpénica (14,5%) y compuestos alifáticos (2,2%). Los principales constituyentes del aceite esencial de M. officinalis fueron citral (47,2%), óxido de cariofileno (10,2%), citronelal (5,4%), geraniol (6,6%), acetato de geranilo (4, 1%), y β-cariofileno (3,8%). El aceite esencial mostró una actividad antiproliferativa significativa contra las líneas celulares de cáncer MOLT-4, MCF-7 y NCI-H460, con valores GI50 de <5, 6±2 y 31±17 µg/mL, respectivamente. Los resultados revelaron que el aceite esencial de M. officinalis L. tiene potencial como agente terapéutico contra el cáncer.Palabras clave: Melissa officinalis L.; Aceite esencial; Agentes antiproliferativos; MOLT-4; MCF-7; NCI-H460 Recibido |

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“…Beyond, citral attracted scientists' attention towards its anticancer properties in a variety of cancer types, such as melanoma [259], colon cancer [260], and breast cancer [261]. Bayala and co-authors provided evidence about Cymbopogon citratus and Cymbopogon gigantescus essential oil cytotoxic activity, which have citral as its major component and significantly decreased prostate and glioblastoma cancer cell survival [262].…”

Section: Citralmentioning

confidence: 99%

Terpenoids, Cannabimimetic Ligands, beyond the Cannabis Plant

et al. 2020

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Medicinal use of Cannabis sativa L. has an extensive history and it was essential in the discovery of phytocannabinoids, including the Cannabis major psychoactive compound—Δ9-tetrahydrocannabinol (Δ9-THC)—as well as the G-protein-coupled cannabinoid receptors (CBR), named cannabinoid receptor type-1 (CB1R) and cannabinoid receptor type-2 (CB2R), both part of the now known endocannabinoid system (ECS). Cannabinoids is a vast term that defines several compounds that have been characterized in three categories: (i) endogenous, (ii) synthetic, and (iii) phytocannabinoids, and are able to modulate the CBR and ECS. Particularly, phytocannabinoids are natural terpenoids or phenolic compounds derived from Cannabis sativa. However, these terpenoids and phenolic compounds can also be derived from other plants (non-cannabinoids) and still induce cannabinoid-like properties. Cannabimimetic ligands, beyond the Cannabis plant, can act as CBR agonists or antagonists, or ECS enzyme inhibitors, besides being able of playing a role in immune-mediated inflammatory and infectious diseases, neuroinflammatory, neurological, and neurodegenerative diseases, as well as in cancer, and autoimmunity by itself. In this review, we summarize and critically highlight past, present, and future progress on the understanding of the role of cannabinoid-like molecules, mainly terpenes, as prospective therapeutics for different pathological conditions.

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Antiproliferative and apoptosis inducing effects of citral via p53 and ROS-induced mitochondrial-mediated apoptosis in human colorectal HCT116 and HT29 cell lines

Cited by 59 publications

References 66 publications

Mechanisms associated to apoptosis of cancer cells by phenolic extracts from two canned common beans varieties (Phaseolus vulgarisL.)

Mechanisms associated to apoptosis of cancer cells by phenolic extracts from two canned common beans varieties (Phaseolus vulgarisL.)

In vitro antiproliferative activity of Melissa officinalis L (Lamiaceae) leaves essential oil

Terpenoids, Cannabimimetic Ligands, beyond the Cannabis Plant

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