Antiproliferative and Apoptosis Inducing Effects of Non-Polar Fractions from Lawsonia inermis L. in Cervical (HeLa) Cancer Cells

Kumar, Manish; Kaur, Paramjeet; Kumar, Subodh; Kaur, Satwinderjeet

doi:10.1007/s12298-015-0285-3

Cited by 16 publications

(9 citation statements)

References 76 publications

(77 reference statements)

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“…In literature, few articles tested a cytotoxic effect of essential oil of Henna; therefore, many studies explore a diverse solvent extraction and the difference of their effect. Then; Kumar et al [37] isolated two non-polar fractions from Lawsonia inermis with hexane (Hex-LI) and with chloroform (CHCl3-LI) which were explored for their anti-proliferative potential against human cell lines (HeLa, MCF-7, A549 et C6). They showed considerable cytotoxicity effects against HeLa with Hex-LI (IC50 = 382.44 μg/mL) and with CHCL3-LI (IC50 = 741.44 μg/mL).…”

Section: Discussionmentioning

confidence: 99%

Lawsonia inermis essential oil: extraction optimization by RSM, antioxidant activity, lipid peroxydation and antiproliferative effects

Elaguel¹,

Kallel²,

Gargouri

et al. 2019

Lipids Health Dis

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BackgroundThe present study was focused on the optimization of yield of the essential oil extraction from leaves of Lawsonia inermis, and the determination of chemical composition, antioxidant activities, and lipid peroxydation and antiproliferative effects.MethodsHenna essential oil (HeEO) were extracted by hydrodistillation; the identification of the chemical composition were done by GC/MS method. HeEO was analyzed for antioxidant power in: (1) chemical system by the DPPH test, the ABTS test and the total antioxidant activity test; and (2) in biological system by lipid peroxydation tests (MDA and DC) in cells culture. The cytotoxicity effects of HeEO were assessed using MTT assay against Raji and HeLa cell lines.ResultsThe optimal extraction yield was 6.8 g/100 g d.b. HeEO showed a remarkable anti-oxidant activities including DDPH (42%), ABTS (87%) and the power of ammonium phosphomolybdate (2992 ± 230 mg of HeEO by equivalent to 1 mg of vitamin C in terms of total antioxidant power).ConclusionBeyond notable antioxidant activities of the HeEo, our results showed a significant decrease in the production of ERO in the Raji cell line. The anti-tumor power of the Henna essential oil shows an interesting cytotoxicity effect (IC50 at 0.26 μg/mL for Raji and at 1.43 μg/mL for HeLa) with a total mortality percentage reaching 60%, for both.

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Section: Discussionmentioning

confidence: 99%

Lawsonia inermis essential oil: extraction optimization by RSM, antioxidant activity, lipid peroxydation and antiproliferative effects

Elaguel¹,

Kallel²,

Gargouri

et al. 2019

Lipids Health Dis

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“…Dasgupta et al [50] reported anticarcinogenic activity of Henna leaves against benzo(a)pyrene induced forestomach as well as against 7,12 dimethylbenz(a)anthracene (DMBA)-initiated and croton oil-promoted skin papillomagenesis. In our previous reports [51, 52], we reported extract/fractions of L. inermis with antioxidant, antiproliferative and apoptosis inducing activity. Since But-LI fraction was found to exhibit high antioxidant activity and is rich in various polyphenolic phytoconstituents viz .…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and hepatoprotective potential of Lawsonia inermis L. leaves against 2-acetylaminofluorene induced hepatic damage in male Wistar rats

Kumar

Kaur

Chandel

et al. 2017

BMC Complement Altern Med

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Background Lawsonia inermis (Lythraceae) is an ethnomedicinal plant, traditionally known for curing several ailments such as skin diseases, bacterial infections, jaundice, renal lithiases and inflammation etc. The present work deals with assessment of in vitro antioxidant and in vivo hepatoprotective potential of butanolic fraction (But-LI) of Lawsonia inermis L. leaves.MethodsAntioxidant activity was evaluated using deoxyribose degradation, lipid peroxidation inhibition and ferric reducing antioxidant power (FRAP) assay. In vivo protective potential of But-LI was assessed at 3 doses [100, 200 & 400 mg/kg body weight (bw)] against 2-acetylaminofluorene (2-AAF) induced hepatic damage in male Wistar rats.ResultsBut-LI effectively scavenged hydroxyl radicals in deoxyribose degradation assay (IC50 149.12 μg/ml). Fraction also inhibited lipid peroxidation and demonstrated appreciable reducing potential in FRAP assay. Treatment of animals with 2-AAF resulted in increased hepatic parameters such as SGOT (2.22 fold), SGPT (1.72 fold), ALP (5.68 fold) and lipid peroxidation (2.94 fold). Different concentration of But-LI demonstrated pronounced protective effects via decreasing levels of SGOT, SGPT, ALP and lipid peroxidation altered by 2-AAF treatment. But-LI administration also restored the normal liver architecture as evident from histopathological studies.ConclusionsThe present experimental findings revealed that phytoconstituents of Lawsonia inermis L. possess potential to effectively protect rats from the 2-AAF induced hepatic damage in vivo possibly by inhibition of reactive oxygen species and lipid peroxidation.

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“…Different research groups evaluate cell death using either cell clonogenic assay (CA) or PI (Kumar et al 2015) assay. Some others use MTT assay (Gehl et al 1998;Kaminska et al 2012).…”

Section: Resultsmentioning

confidence: 99%

Different Cell Viability Assays Reveal Inconsistent Results After Bleomycin Electrotransfer In Vitro

et al. 2015

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The aim of this study was to compare different and commonly used cell viability assays after CHO cells treatment with anticancer drug bleomycin (20 nM), high voltage (HV) electric pulses (4 pulses, 1200 V/cm, 100 µs, 1 Hz), and combination of bleomycin and HV electric pulses. Cell viability was measured using clonogenic assay, propidium iodide (PI) assay, MTT assay, and employing flow cytometry modality to precisely count cells in definite volume of the sample (flow cytometry assay). Results showed that although clonogenic cell viability drastically decreased correspondingly to 57 and 3 % after cell treatment either with HV pulses or combination of bleomycin and HV pulses (bleomycin electrotransfer), PI assay performed ~15 min after the treatments indicated nearly 100 % cell viability. MTT assay performed at 6-72 h time points after these treatments revealed that MTT cell viability is highly dependent on evaluation time point and decreased with later evaluation time points. Nevertheless, in comparison to clonogenic cell viability, MTT cell viability after bleomycin electrotransfer at all testing time points was significantly higher. Flow cytometry assay if used at later times, 2-3 days after the treatment, allowed reliable evaluation of cell viability. In overall, our results showed that in order to estimate cell viability after cell treatment with combination of the bleomycin and electroporation the most reliable method is clonogenic assay. Improper use of PI and MTT assays can lead to misinterpretation of the experimental results.

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Antiproliferative and Apoptosis Inducing Effects of Non-Polar Fractions from Lawsonia inermis L. in Cervical (HeLa) Cancer Cells

Cited by 16 publications

References 76 publications

Lawsonia inermis essential oil: extraction optimization by RSM, antioxidant activity, lipid peroxydation and antiproliferative effects

Lawsonia inermis essential oil: extraction optimization by RSM, antioxidant activity, lipid peroxydation and antiproliferative effects

Antioxidant and hepatoprotective potential of Lawsonia inermis L. leaves against 2-acetylaminofluorene induced hepatic damage in male Wistar rats

Different Cell Viability Assays Reveal Inconsistent Results After Bleomycin Electrotransfer In Vitro

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