Antiproliferative and antioxidant potential of hesperetin against benzo(a)pyrene-induced lung carcinogenesis in Swiss albino mice

Bodduluru, Lakshmi Narendra; Kasala, Eshvendar Reddy; Barua, Chandana Choudhury; Karnam, Kalyani; Dahiya, Vicky; Ellutla, Maheswara

doi:10.1016/j.cbi.2015.10.020

Cited by 62 publications

(25 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TNF-α was considered a critical pro-inflammatory cytokine in tumors, and increasing evidence suggested that TNF-α may act as an endogenous tumor promoter (35,36). Results of the present study demonstrated a significant decrease (P<0.05) in TNF-α level by baicalein and baicalein-cisplatin combination treatments.…”

Section: Discussionsupporting

confidence: 53%

Regulation of cytotoxicity and apoptosis-associated pathways contributes to the enhancement of efficacy of cisplatin by baicalein adjuvant in human A549 lung cancer cells

et al. 2017

View full text Add to dashboard Cite

Abstract. Scutellaria baicalensis (SB; Chinese name, huangqin) is widely used in Chinese medicine as a traditional adjuvant in the chemotherapy of lung and liver cancer. Baicalein is one of the bioactive flavonoid components isolated from the root of SB. The present study aimed to observe the effect of baicalein, in combination with platin-based systemic chemotherapy (cisplatin), on cytotoxicity and apoptosis of human A549 lung cancer cells. The cell cultures were treated with baicalein, cisplatin, or a combination of the two. Cell viability and cytotoxicity was assayed by XTT, and cell apoptosis was measured by flow cytometry. The apoptosis-associated proteins were detected by western blot analysis. The cytokines in the culture supernatant were detected by ELISA. The present study revealed that cisplatin and the baicalein-cisplatin combination inhibited viability and promoted cytotoxicity of A549 cells. Cisplatin, baicalein and baicalein-cisplatin combination treatments were effective in the promotion of apoptosis of A549 cells. Baicalein and baicalein-cisplatin combination treatments also inhibited B cell lymphoma-2 (Bcl-2) and increased Bcl-2-associated X protein (Bax) expression. Additionally, cisplatin, baicalein and the baicalein-cisplatin combination promoted caspase-3 expression. Furthermore, the baicalein-cisplatin combination suppressed the secretion of interleukin-6, and baicalein and the combination of baicalein cisplatin decreased the secretion of tumor necrosis factor-α of A549 cells. The present study concluded that baicalein combined with cisplatin induced cytotoxicity and apoptosis of A549 cells, and such activity may be associated with the regulation of Bcl-2, Bax and caspase-3, indicating a promising alternative method for lung cancer.

show abstract

Section: Discussionsupporting

confidence: 53%

Regulation of cytotoxicity and apoptosis-associated pathways contributes to the enhancement of efficacy of cisplatin by baicalein adjuvant in human A549 lung cancer cells

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Despite, HspNPs has shown lower SOD activity compared to free Hsp, both HspNPs and free Hsp has increased SOD levels comparison to control group in a dose dependent manner. SOD, CAT, GPx, GST and GR are critical intracellular oxidants which combats the reactive oxygen species-induced cell death and tissue damage [53]. Our findings revealed that untreated C6 glioma cells has shown decreased activity of SOD enzyme.…”

Section: Sodmentioning

confidence: 62%

Comparative evaluation of hesperetin loaded nanoparticles for anticancer activity against C6 glioma cancer cells

Ersöz

Erdemir

Duranoğlu

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

The aim of this study was to evaluate anti-cancer properties of hesperetin (Hsp) and hesperetin-loaded poly(lactic-co-glycolic acid) nanoparticles (HspNPs) for glioblastoma treatment. Nanoparticles prepared by single emulsion method had a size of less than 300 nm with 70.7 ± 3.9% reaction yield and 26.4 ± 1.1% Hsp loading capacity. Treatment of C6 glioma cells with HspNPs for 24 and 48 h resulted in dose-and time-dependent decrease in cell viability, with approximate IC 50 of 28 and 21 lg/mL, respectively (p ¼ .036 for 24 h, p ¼ .025 for 48 h). The percentage of PCNA positive cells decreased to 20% and 10%, respectively, for Hsp-and HspNP-treated cells at concentration of 100 mg/mL. Treatment with increasing concentrations of HspNPs (25, 50, 75 and 100 mg/mL) resulted in 9.1-, 7-, 12.5-and 12.7-fold in increase in apoptotic cell number. Optimum doses of Hsp and HspNPs were found to increase oxidative damage in C6 glioma cells. MDA levels, an indicator of lipid peroxidation, were found to be significantly elevated at 75 and 100 mg/mL exposure concentration of HspNPs with (p ¼ .002) and (p ¼ .018), respectively for 48-h treatment. The results obtained with this study showed biocompatible polymeric nanoparticle systems has great advantages to enhance anti-cancer activity and poor solubility of therapeutic agents. Overall our findings suggest that Hsp-loaded PLGA nanoparticle systems showed significant anti-cancer activity and HspNPs could be used as promising novel anti-cancer agent. ARTICLE HISTORY

show abstract

“…Even the previous studies in this regard ratify our observations. In breast, lung and other cancer indications; antioxidants provided protective effect against abnormalities in GSH, Vitamins C and E (Lakshmi and Subramanian, 2014; Bodduluru et al, 2015). Therefore, honokiol is a promising agent in preventing oxidative stress-induced breast cancer associated anomalies.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, the slides were stained by dipping in 2% eosin solution and washed twice with absolute alcohol. The slide was air dried and then mounted with DPX (mounting media) and covered with glass slip (Bodduluru et al, 2015). …”

Section: Methodsmentioning

confidence: 99%

Chemopreventive Activity of Honokiol against 7, 12 - Dimethylbenz[a]anthracene-Induced Mammary Cancer in Female Sprague Dawley Rats

Wang

Zhang

2017

Front. Pharmacol.

View full text Add to dashboard Cite

Breast cancer is a predominant cause of death in women across the globe. Chemoprevention by using natural, dietary or synthetic products has been appearing to be a fascinating approach to combat the growing burden of breast cancer. In the current study, we intended to explore the mechanisms of chemopreventive action of honokiol against 7, 12 - dimethylbenz[a]anthracene (DMBA)-induced mammary cancer in female Sprague Dawlely (SD) rats. We induced mammary cancer in SD rats by administering single dose of DMBA (80 mg/kg) through intra gastric route. Chemopreventive effects of honokiol (80 mg/kg, i.p.) were confirmed from its ameliorating effect on the DMBA-induced anomalies such as liver marker enzymes, Phases I and II metabolizing enzymes and oxidative stress markers. Further, honokiol reversed the DMBA-induced abnormalities in inflammatory cytokines levels and serum tumor markers. Additionally, histopathological examination of mammary tissue and protein expression analysis of NF-κB revealed that honokiol is effective against DMBA-induced mammary cancer. In summary, the results of our study support the chemopreventive feature of honokiol in mammary cancer.

show abstract

Antiproliferative and antioxidant potential of hesperetin against benzo(a)pyrene-induced lung carcinogenesis in Swiss albino mice

Cited by 62 publications

References 28 publications

Regulation of cytotoxicity and apoptosis-associated pathways contributes to the enhancement of efficacy of cisplatin by baicalein adjuvant in human A549 lung cancer cells

Regulation of cytotoxicity and apoptosis-associated pathways contributes to the enhancement of efficacy of cisplatin by baicalein adjuvant in human A549 lung cancer cells

Comparative evaluation of hesperetin loaded nanoparticles for anticancer activity against C6 glioma cancer cells

Chemopreventive Activity of Honokiol against 7, 12 - Dimethylbenz[a]anthracene-Induced Mammary Cancer in Female Sprague Dawley Rats

Contact Info

Product

Resources

About