Comparative evaluation of hesperetin loaded nanoparticles for anticancer activity against C6 glioma cancer cells

Abstract

The aim of this study was to evaluate anti-cancer properties of hesperetin (Hsp) and hesperetin-loaded poly(lactic-co-glycolic acid) nanoparticles (HspNPs) for glioblastoma treatment. Nanoparticles prepared by single emulsion method had a size of less than 300 nm with 70.7 ± 3.9% reaction yield and 26.4 ± 1.1% Hsp loading capacity. Treatment of C6 glioma cells with HspNPs for 24 and 48 h resulted in dose-and time-dependent decrease in cell viability, with approximate IC 50 of 28 and 21 lg/mL, respectively (p ¼ .036 for 24 h, p ¼ .025 for 48 h). The percentage of PCNA positive cells decreased to 20% and 10%, respectively, for Hsp-and HspNP-treated cells at concentration of 100 mg/mL. Treatment with increasing concentrations of HspNPs (25, 50, 75 and 100 mg/mL) resulted in 9.1-, 7-, 12.5-and 12.7-fold in increase in apoptotic cell number. Optimum doses of Hsp and HspNPs were found to increase oxidative damage in C6 glioma cells. MDA levels, an indicator of lipid peroxidation, were found to be significantly elevated at 75 and 100 mg/mL exposure concentration of HspNPs with (p ¼ .002) and (p ¼ .018), respectively for 48-h treatment. The results obtained with this study showed biocompatible polymeric nanoparticle systems has great advantages to enhance anti-cancer activity and poor solubility of therapeutic agents. Overall our findings suggest that Hsp-loaded PLGA nanoparticle systems showed significant anti-cancer activity and HspNPs could be used as promising novel anti-cancer agent. ARTICLE HISTORY

Hesperetin Promotes Cisplatin−Induced Apoptosis of Gastric Cancer In Vitro and In Vivo by Upregulating PTEN Expression

“…Wang et al reported that hesperetin was likely to act as an accelerator for the anti-cancer effect of DDP in lung adenocarcinoma ( Wang et al, 2019 ). Although there is growing evidence that hesperetin induces apoptosis in different types of cancer cells ( Ersoz et al, 2019 ; Li et al, 2019 ), whether hesperetin can enhance the chemical sensitivity of GC to DDP, and its specific mechanism, remains unclear.…”

Nanocrystallized Oleanolic Acid Better Inhibits Proliferation, Migration and Invasion in Intracranial Glioma via Caspase-3 Pathway

“…Both pathways can initiate caspase family proteins, including Caspase-3, Caspase-7, Caspase-8, etc. These proteins activate deoxyribonuclease, leading to chromosomal DNA fragmentation during apoptosis [19,20].…”

“…In the current study, U87 glioma cells were treated with OA nano or normal OA at different concentrations (10,20,30,40,50,60,70, 80 μmol/L) during designated time period to verify their therapeutic effects. In OA nano group, 50% of U87 glioma cells lost their activity (IC 50 ) at 35 μmol/L (Fig.…”

Hesperetin as an adjuvant augments protective anti‐tumour immunity responses in B16F10 melanoma by stimulating cytotoxic CD8+ T cells

“…It is derived from the young citrus fruit of the genus rutaceae . Recent studies showed that HES had antioxidant and anti‐cancer effects and it also modulated immune responses . So far, there has been no research on hesperetin as an adjuvant.…”

“…16 Recent studies showed that HES had antioxidant and anti-cancer effects and it also modulated immune responses. [17][18][19] So far, there has been no research on hesperetin as an adjuvant. Therefore, we investigated the potential use of hesperetin as a B16F10 melanoma vaccine adjuvant.…”