Antiproliferative Activity, Proapoptotic Effect, and Cell Cycle Arrest in Human Cancer Cells of Some Marine Natural Product Extract

Abstract

Bioactive constituents of numerous marine organisms have been investigated recently for their preclinical and clinical anticancer activity. Three marine organisms: black-spotted sea cucumber: Pearsonothuria graeffei (Pg), lollyfish: Holothuria atra (Ha), and sea hare: Aplysia dactylomela (Ad), were collected during winter 2019 from Gulf of Aqaba, Red Sea, Egypt, and macerated with ethanol into three different extracts: PgE, HaE, and AdE, where each was in vitro assessed for its antiproliferative and proapoptotic properties on HepG2, HCT-116, and MCF-7 cancer cells. PgE dose-dependently inhibited the growth of HepG2, HCT-116, and MCF-7 cells within IC50 values 16.22, 13.34, and 18.09 μg/mL, respectively, while the IC50 values for the antiproliferative activity of HaE were 12.48, 10.45, and 10.36 μg/mL, respectively, and the IC50 values of AdE were 6.51, 5.33, and 6.87 μg/mL, respectively. All extracts were found to induce G0/G1 cell cycle arrest for HepG2 cells side by side with their inhibition of CDK2 on all three cell lines while all extracts were also showed to induce apoptosis in HepG2 cell line at pre-G1 phase supplemented by their anticancer activity via proapoptotic protein Bax, caspase-3, and cleavage PARP increase, and antiapoptotic protein Bcl-2 downturn. Moreover, necrosis has been relatively noticed in HepG2 cell line as an additional anticancer activity for each extract. Our data introduced three ethanolic marine extracts as natural chemotherapeutic agents to be further developed for cancer control.

Cell-Free Supernatant of Odoribacter splanchnicus Isolated From Human Feces Exhibits Anti-colorectal Cancer Activity

“…Because cancer cells are highly proliferative, anti-proliferative activity has been investigated as one of the major targets in the development of anti-cancer drugs ( Dembic, 2020 ). For instance, the anti-proliferative activities of traditional herbal and marine derivative products were screened to assess their potential anti-cancer effects and consequently develop anti-cancer drugs ( da Silva et al, 2019 ; Cui et al, 2020 ). Therefore, to identify anti-CRC candidates from the gut microbiota, we examined the anti-proliferative activity of these candidates against HCT 116 cells in vitro .…”

Acanthaster planci Inhibits PCSK9 and Lowers Cholesterol Levels in Rats

“…Various studies demonstrated the cell death-inducible effect of marine organisms on HepG2 cells, such as mesohyl of a sponge, Hemimycle arabica [ 38 ]. In addition, the extract prepared from sea cucumber Pearsonothuria graeffei , lollyfish Holothuria atra , and sea hare Aplysia dactylomela also produced the cytotoxicity activity on HepG2 cell line with IC 50 of 16.22, 12.48, and 6.51 µg/mL, respectively [ 39 ]. In contrast, the extract and fractions of A. planci did not produce any cytotoxicity activity on the HepG2 cell line ( Figure 1 and Figure 3 ).…”

The photosensitizing activity of different photosensitizers irradiated with sunlight against aquatic larvae of Culex pipiens L. (Diptera: Culicidae)

“…Therefore, there is a need to devise new techniques to eliminate these problematic creatures. Recently, the main goal for scientists is to use of natural products and ecofriendly solutions for eliminate these problematic creatures (Ibrahim et al, 2017;Cui et al, 2020;Metwally et al, 2020;Attia et al, 2021). One of these ecofriendly solutions is photosensitizers irradiation.…”