Antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A on human melanoma A375 cells

Liu, Bo; Li, Chunyang; Bian, He-jiao; Min, Mingwei; Chen, Longfei; Bao, Ji

doi:10.1016/j.abb.2008.12.003

Cited by 92 publications

(50 citation statements)

References 26 publications

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“…The amount of oligonucleosomal-sized fragments in HCT-15 cells treated with AIL were increased with increase in concentration of AIL from 25 µg/ml to 100 µg/ml. The results obtained are in agreement with the effect of Con A and Con Br on HCT-15, A 375 and leukemic cell lines [36][37] . Trypan blue exclusion assay was performed to check the viability percentage (%) of colon cancer cell line HCT-15 against AIL at series of concentration.…”

Section: Resultssupporting

confidence: 81%

Mechanistic Studies of in vitroAnti-Proliferative Potential of Arisaena intermedium Lectin

Gautam¹,

Kaur²,

Thakur³

et al. 2016

IJLSSR

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ABSTRACT-Aberrant glycosylation has been recognized as hallmark of cancer. Exploiting differences in glycosylation between malignant and healthy tissues offers excellent opportunities to identify sensitive and specific cancer biomarkers. Plant lectins have demonstrated the ability to specifically agglutinate malignant transformed cells. Lectins are sugar binding proteins or glycoprotein of non-immune origin which agglutinate cells or precipitate glycol-conjugates. Some lectins shown to the anti-proliferative effect on cancer cells. A wide scope of this application of lectins is that it can be used for diagnosis as well as therapeutics of cancer. The objective of the present study was to purify a lectin from tubers of Arisaema intermedium and evaluate in vitro anti-proliferative potential towards HCT-15, a human colon cancer cell line. The present study was conceived as an offshoot to the ongoing work on lectins in our laboratory. The already reported Arisaema intermedium (AIL) lectin was purified on asialofetuin linked amino-activated silica bead matrix. The purity of the affinity purified lectin was ascertained by SDS-PAGE, pH-8.3. The lectin activity was assessed by hemagglutination and protein concentration was determined by Lowry's method. The cytotoxicity of AIL towards HCT-15 was evaluated by MTT assay. The mechanism of anti-proliferative effect was assessed by evaluation of cell morphology, trypan blue exclusion assay, DNA fragmentation and nucleic acid content determination.

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Section: Resultssupporting

confidence: 81%

Mechanistic Studies of in vitroAnti-Proliferative Potential of Arisaena intermedium Lectin

Gautam¹,

Kaur²,

Thakur³

et al. 2016

IJLSSR

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“…Apoptosis is an intrinsic cell death mechanism that plays an important role in the maintenance of healthy tissue and is characterized by cell contraction, chromatin condensation, DNA fragmentation, and activation of caspases (Salvesen and Dixit, 1997). Therefore, searching for agents that trigger apoptosis of tumor cells has become an attractive strategy in anticancer drug discovery (Reed, 2003;Liu et al, 2009aLiu et al, , 2009b. One of the major findings in this study is that BJcuL induced apoptotic cell death in gastric carcinoma MKN45 and AGS cell lines, as reported by other lectins.…”

Section: T R a C Tmentioning

confidence: 54%

BJcuL, a lectin purified from Bothrops jararacussu venom, induces apoptosis in human gastric carcinoma cells accompanied by inhibition of cell adhesion and actin cytoskeleton disassembly

Nolte

Damasio

Baréa

et al. 2012

Toxicon

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“…In this regard, several plant lectins, including Phaseolus vulgaris lectin, Concanavalin-A (ConA) and lectins from Astragalus membranaceus and Momordica charantia, induce apoptotic cell death in cancer cells that originate from different sources and tissues [26][27][28][29][30] . Additionally, ConA has been reported to elicit autophagic cell death in HCC [31] .…”

Section: Discussionmentioning

confidence: 99%

Abrus agglutinin suppresses human hepatocellular carcinoma in vitro and in vivo by inducing caspase-mediated cell death

et al. 2014

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Aim: Abrus agglutinin (AGG) from the seeds of Indian medicinal plant Abrus precatorius belongs to the class II ribosome inactivating protein family. In this study we investigated the anticancer effects of AGG against human hepatocellular carcinoma in vitro and in vivo. Methods: Cell proliferation, DNA fragmentation, Annexin V binding, immunocytofluorescence, Western blotting, caspase activity assays and luciferase assays were performed to evaluate AGG in human liver cancer cells HepG2. Immunohistochemical staining and TUNEL expression were studied in tumor samples of HepG2-xenografted nude mice.

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Antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A on human melanoma A375 cells

Cited by 92 publications

References 26 publications

Mechanistic Studies of in vitroAnti-Proliferative Potential of Arisaena intermedium Lectin

Mechanistic Studies of in vitroAnti-Proliferative Potential of Arisaena intermedium Lectin

BJcuL, a lectin purified from Bothrops jararacussu venom, induces apoptosis in human gastric carcinoma cells accompanied by inhibition of cell adhesion and actin cytoskeleton disassembly

Abrus agglutinin suppresses human hepatocellular carcinoma in vitro and in vivo by inducing caspase-mediated cell death

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