Antiproliferation effect of sulforaphene isolated from radish (Raphanus sativus L.) seeds on A549 cells

Abstract

Sulforaphene (SFE), a major isothiocyanate in radish seeds, is a close chemical relative of sulforaphane (SFA) isolated from broccoli seeds and florets. The anti-proliferative mechanisms of SFA against cancer cells have been well investigated, but little is known about the potential anti-proliferative effects of SFE. In this study, we showed that SFE purified from radish seeds inhibited the growth of six cancer cell lines (A549, CHO, HeLa, Hepa1c1c7, HT-29, and LnCaP), with relative half maximal inhibitory concentration values ranging from 1.37 to 3.31 μg/mL. Among the six cancer cell lines, SFE showed the greatest growth inhibition against A549 lung cancer cells, where it induced apoptosis by changing the levels of poly(ADP-ribose) polymerase and caspase-3, -8, and -9. Our results indicate that SFE from radish seeds may have significant anti-proliferative potency against a broad range of human cancer cells via induction of apoptosis.

Effects of Glucosinolate-Enriched Red Radish (Raphanus sativus) on In Vitro Models of Intestinal Microbiota and Metabolic Syndrome-Related Functionalities

“…Among the health-promoting biomolecules mentioned before, GSLs stand out due to their antifungal, antibacterial, antioxidant, antimutagenic, hepatoprotective, nephroprotective, antidiabetic, anti-inflammatory, and antiproliferative activities. , These sulfur-rich anionic secondary metabolites are classified into three groups based on their amino acid precursors: aliphatic, aromatic, and indolic GSLs . GSLs usually undergo a process of degradation by the myrosinase enzyme (EC 3.2.1.147), giving the bioactive molecules isothiocyanates (ITCs) .…”

Antioxidant Activity of Radish Seed Oil and the Quality and Storage Characteristics of Pork Patties with Added Radish Seed Oil

“…Radish seeds contain health-promoting compounds such as sulforaphene and glucosinolates, which are found in cruciferous vegetables ( Gutiérrez and Perez, 2004 ). Sulforaphene is a bioactive compound with anticancer effects that induces cell apoptosis and which has a similar chemical structure to sulforaphane isolated from broccoli seeds ( Lim et al, 2020 ). Glucosinolates are organic compounds that are known for their powerful antimicrobial and anticancer properties.…”

Comparative analysis of isothiocyanates in eight cruciferous vegetables and evaluation of the hepatoprotective effects of 4-(methylsulfinyl)-3-butenyl isothiocyanate (sulforaphene) from daikon radish (Raphanus sativus L.) sprouts

“…The anticancer effects on human breast cancer cells, human lung cancer cells, human hepatocarcinoma cells, and human oesophageal cancer cells have been previously reported. [51][52][53][54][55] In addition, anti-adipogenic effects on mouse adipocytes and anti-neuroinflammatory effects in model rats with Alzheimer's disease have been described. 56,57 The present study showed that the hepatoprotective effect of sulforaphene was nearly equal to that of sulforaphane and the amount of sulforaphene in daikon radish sprouts was higher than that of sulforaphane in broccoli sprouts.…”