Antiproliferation Effect and Mechanism of Prostate Cancer Cell Lines as Affected by Isoflavones from Soybean Cake

Abstract

The objectives of this study were to determine the antiproliferation effect of prostate cancer cell lines LNCaP and PC-3 as affected by 4 isoflavone fractions prepared from soybean cake and isoflavone standards genistein and daidzein. With the MTT test, most treatments were effective in inhibiting prostate cancer cell growth at a low dose of 5 and 10 mug/mL. In cell cycle analysis, the fractions of aglycon, a mixture of acetylglucoside and aglycon, as well as genistein and a combination of genistein and daidzein standards exhibited a high G2/M ratio for LNCaP, as did the acetylglucoside, genistein and a combination of genistein and daidzein standards for PC-3. Results of Western blotting revealed an increase in p53 protein expression of LNCaP following treatments of the aglycon fraction, genistein and a combination of genistein and daidzein standards. However, all the treatments did not affect Bcl-2 protein expression significantly in both LNCaP and PC-3 cells. A decline in cyclin B1 expression of LNCaP was observed for all the treatments, with the mixture of acetylglucoside and aglycon possessing the most pronounced effect. But for PC-3, a decrease in cyclin B1 expression was shown for all the isoflavones, with the exception of malonylglucoside, glucoside and acetylglucoside fractions. The outcome of this study may provide a basis for possible production of functional food in the future with soybean cake as raw material.

Synergistic chemoprotective mechanisms of dietary phytoestrogens in a select combination against prostate cancer

“…Thus, this study demonstrates that innovative combination of phytoestrogens can elicit significant synergistic effects resulting in improved anticancer efficacy at physiologically achievable concentrations. Though previous studies have shown that G, B and Q individually inhibit proliferation of prostate cancer cells in vitro [30][31][32][33], but their combination effect has not been investigated. It has been shown that multiple flavonoid mixture can alter the pharmacokinetics to increase the bioavailability of individual flavonoids [34].…”

Novel isoflavone diglycoside in groundnut (Apios americana Medik)

“…Genistin, a glycoside that contains genistein as its aglycone skeleton, is hydrolysed by enzymes in saliva and the intestinal mucosa as well as by enterobacterial b-glucosidase to produce genistein (Day et al, 2000;Hur, Lay, Beger, Freeman, & Rafii, 2000;Ismail & Hayes, 2005;Izumi et al, 2000;Slavin, Karr, Ilutchins, & Lampe, 1998). Several reports have shown that genistein serves a wide variety of functions including antioxidative, anticarcinogenic, and antiallergic activities (Alexandrakis et al, 2003;Chacko et al, 2007;Chung et al, 2006Chung et al, , 2008Hanasaki et al, 1994;Liu et al, 2005;Wang et al, 2009;Zhang et al, 2004), suggesting that the groundnut is a beneficial crop with potential health effects. These results indicate that groundnuts can be an important source of isoflavones, with comparable significance to soybeans, which are now recognised as the primary dietary isoflavone source.…”

“…Recent reports have shown that soybean isoflavones possess oestrogenlike activity and reduce osteoporosis risks (Arjmandi et al, 1996;Zhang, Ng, & Luo, 2007). Additionally, isoflavones have been proven to exert various pharmacological actions (Alexandrakis et al, 2003;Chacko et al, 2007;Chung, Hogan, Zhang, Rainey, & Zhou, 2008;Chung et al, 2006;Hanasaki, Ogawa, & Fukui, 1994;Liu, Chang, & Wiesenborn, 2005;Wang, Wang, Lu, Kao, & Chen, 2009;Zhang, Xie, Lee, & Binns, 2004) such as carcinostatic (anticarcinogenic and anti-proliferative), antihypertensive, antioxidative, and antiallergic activities, gaining increasing interest as functional components of soybeans.…”

Isolation and Identification of Chlorinated Genistein from Actinoplanes sp. HBDN08 with Antioxidant and Antitumor Activities

“…Compound 1 (Figure 1) was isolated as an offwhite amorphous solid. Its molecular formula was established to be C 15 H and 13 C NMR data revealed that compound 1 was an isoflavone. Its 1 H NMR spectrum showed a hydrogen-bonded hydroxyl group at δ 12.89, due to the C-5 hydroxyl proton, and a characteristic singlet for isoflavone at δ 8.32 corresponding to H-2.…”