Antiplasmodial Evaluation of One Compound from &lt;i&gt;Calophyllum flavoranulum&lt;/i&gt;

Abbas, Jamilah; Syafruddin, Syafruddin

doi:10.22146/ijc.21257

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…Berdasarkan penelitian yang dilakukan oleh Prasiwi dan kawan-kawan, didapatkan dosis efektif untuk mengobati penyakit malaria pada mencit yaitu sebesar 0,084 g/KgBB dimana didapatkan data persentase penghambatan pertumbuhan Plasmodium berghei sebesar 54,06%. 12 Penelitian lainnya yang telah dilakukan mengenai ekstrak dari tumbuhan herbal seperti ekstrak tumbuhan berbunga Asteraceae dan Rubiaceae families 13 , daun bunga nusa indah (Mussaenda erythrophylla) 13 , daun nyamplung (Calophyllum flavoranulum) 14 , daun kedondong hutan (Spondias pinnata) 15 , dan kulit batang gambilo (Gardenia ternifolia) 16 . doi:10.24843.MU.2023.V12.i8.P03 dengan kandungan bahan aktif seperti alkaloid, terpen, antrakuinon, flavonoid, tannin, saponin, dan steroid.…”

Section: Pembahasanunclassified

Uji Efektivitas Kombinasi Ekstrak Daun Sirih Hijau “Piper Betle L” Dan Madu Sebagai Antimalaria Pada Mencit Yang Diinfeksi Plasmodium Berghei

Swastika,

Sri Laksemi,

Damayanti

et al. 2023

eum

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Malaria is an infectious disease caused by the parasite Plasmodium sp. transmitted by the female Anopheles mosquito vector through its bite. Patients infected with malaria worldwide in the last two decades have increased, mainly due to the emergence of strains of Plasmodium falciparum that have become resistant to antimalarial drugs. This has resulted in the emergence of efforts to find alternative medicinal plants that can overcome this problem. One of the plants that have the potential to be developed is Piper betle L, or commonly known as green betel plant. This study aims to determine the effect of green betel leaf extract (Piper betle L) and honey at doses of 50, 100, and 200 mg/kgBW as antimalarials in Balb/c mice infected with Plasmodium berghei. This study is an in vivo experimental study using the Randomized Post-test Only Controlled Group method design using 24 samples of mice which were divided into four groups, one negative control group and three treatment groups. The average degree of parasitemia in the control group was 45.51%, the treatment group at a dose of 50 mg/kgBW was 35.71%, a dose of 100 mg/kgBW was 30.96% and a dose of 200 mg/kgBW was 31.67%. Based on the results of the analysis using One Way ANOVA, it was found that the mean difference between the control and treatment groups with p value <0.001 was found. The results showed that green betel leaf extract (Piper betle L) could inhibit the growth of Plasmodium berghei by looking at the mean difference between the control group and the treatment group and obtained that the average treatment group of 100 mg/kgBW and 200 mg/kgBW were equally effective at inhibiting parasitemia levels. Keywords : Antimalarial, Piper betle L, Degree of Parasitemia

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Section: Pembahasanunclassified

Uji Efektivitas Kombinasi Ekstrak Daun Sirih Hijau “Piper Betle L” Dan Madu Sebagai Antimalaria Pada Mencit Yang Diinfeksi Plasmodium Berghei

Swastika,

Sri Laksemi,

Damayanti

et al. 2023

eum

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“…Abbas and Syafruddin (2010) successfully isolated the compound of xanthon that is fenil kaumarin from Calophyllum flavoranulum from Kalimantan and reported its antiplasmodial activity invivo in mencit that was infected by Plasmodium Berghei. 19 Rissyelly (2010) also reported antiplasmodial activity of ksanton compound namely [2-(3',3'-dimetilalil)-1,3,7-trihidroksiksanton] from Calophyllum canum. 20 The changes in morphology and ultra structure of P. falciparum 3D7 after being exposed to hexane fraction extract C. bicolor might be related to the mechanism and target of given anti plasmodial.…”

Section: Figure 2 Vacuole Membrane Boundary and Hemozoinmentioning

confidence: 99%

ANTI-PLASMODIUM FALCIPARUM IN VITRO ACTIVITY OF CALOPHYLLUM BICOLOR EXTRACT: Morphology and Ultra Structure

et al. 2015

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Background: Anti-malarial resistance of Plasmodium, such as chloroquine becoming a health problem worldwide. This research aims to evaluate the in vitro anti-plasmodium activity of hexane fraction extract of Calophyllum bicolor (C. bicolor) against culture of Plasmodium falciparum (P. falciparum) 3D7 and determine its inhibitor concentration of 50% (IC 50 ); to observe the microscopic changes of P. falciparum after exposed to the hexane fraction of C. bicolor extract and to observe the ultra structure changes of P. falciparum 3D7 after being exposed to hexane fraction of C. bicolor extract. Method: This research applied quasi experimental with post-test only control group design. Anti malarial activity test of hexane fraction of C. bicolor extract towards Plasmodium falciparum 3D7 2% was undertaken over 48 hours with in vitro incubation technique. The level of parasitemia was observed using binocular optical microscope with 1000x magnification by counting the infected erythrocytes with Giemsa color technique. Then it was analyzed to determine the level of inhibitor concentration of 50% (IC 50 ) of the C. bicolor extract. The next step is to observe the changes of parasite's morphologic and ultra structure after the 24 and 48 hours incubation of the parasite P. falciparum 3D7 with hexane fraction of C. bicolor, IC 50 . Then, the morphologic change of P. falciparum 3D7 was observed with optical binocular microscopy and the ultra structure changes with Transmission Electron Microscopy (TEM). The changes of morphologic and ultra structure were analyzed qualitatively. Results: The research revealed that hexane fraction of the C. bicolor extract inhibited the growth of the parasite P. falciparum 3D7 with the value of -49.00±2.54, 12.53±3.13, 23.01±1.10, 27.68±4.23, 48.65±18.71, 70.82±4.67, 80.52±6.17% with the hexane extract concentration of 0, 0.001, 0.01, 0.1, 1, 10, 100, and 1000 µg/mL. This research also found that the inhibitor concentration of 50% (IC 50 ) of hexane fraction of C. bicolor extract was 3.94 µg/mL in 10 -7 which could change the morphologic and ultra structure of P. falciparum 3D7. There were several changes in morphology of the parasite over 24 hour incubation compared with the control group, i.e. the nucleus of young tropozoid became thicker, darker, and smaller; the skizon were condensate, thicker, darker, karyorrhexis and demolition of membrane. Hexane fraction of C. bicolor extract also changes the ultra structure over 24 hour incubation, i.e. vacuole membrane boundary and hemozoin were unclear. Over 48 hours of incubation the nucleus and cytoplasm bigger than those in the control group. Conclusion: Hexane fraction of the C. bicolor extract showed a strong anti plasmodium activity towards the in vitro culture of P. falciparum 3D7 at the level of (IC 50 ) 3.94 µg/mL. The Hexane fraction of C. bicolor extract could change the morphology and ultra structure of P. falciparum 3D7 in vitro.

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“…The scientific study of the genus Calophyllum revealed that it is a rich source of bioactive secondary metabolites showing a wide range of biological activities. Some coumarins from C. flavoranulum showed activity against Plasmodium berghei parasite [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Caloforines A–G, coumarins from the bark of Calophyllum scriblitifolium

et al. 2022

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Antiplasmodial Evaluation of One Compound from <i>Calophyllum flavoranulum</i>

Abstract: 0, 31,33, 40, 30.67, 46 dan 34% pada konsentrasi sampel berturut-turut 1,1x10 -10

Cited by 5 publications

References 15 publications

Uji Efektivitas Kombinasi Ekstrak Daun Sirih Hijau “Piper Betle L” Dan Madu Sebagai Antimalaria Pada Mencit Yang Diinfeksi Plasmodium Berghei

Uji Efektivitas Kombinasi Ekstrak Daun Sirih Hijau “Piper Betle L” Dan Madu Sebagai Antimalaria Pada Mencit Yang Diinfeksi Plasmodium Berghei

ANTI-PLASMODIUM FALCIPARUM IN VITRO ACTIVITY OF CALOPHYLLUM BICOLOR EXTRACT: Morphology and Ultra Structure

Caloforines A–G, coumarins from the bark of Calophyllum scriblitifolium

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