Antiplasmodial activity of Vernonia cinerea Less (Asteraceae), a plant used in traditional medicine in Burkina Faso to treat malaria

Soma, Aboubakar; Sanon, Souleymane; GansanÃ, Adama; Ouattara, Lamoussa Paul; OuÃ©draogo, Noufou; Nikiema, Jean‐Baptiste; Sirima, Sodiomon B.

doi:10.5897/ajpp2016.4703

Cited by 7 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the study of Reddy et al, (2005) 11 the extract period was carried out for 5 days, so it can be concluded that the dose and period of administration of Curcuma xanthorriza Roxb extract in this study was not enough to show significant results. The author used 4 days because 4 days was the minimum time in the study using extracts to get the benefits of the extract 13 . Group 3 (K3) compared to group 4 (K4) has a P-Value>0.05 which means that there is no significant difference in cell injury of liver tissue in that group.…”

Section: Comparison Testmentioning

confidence: 99%

The Effect of Rhizome Extract of Curcuma (Curcuma xanthorriza Roxb) for Cell Injury in Histopathology of Liver Tissue of Male White Mice (Mus musculus L.) Strain BALB/C Infected by Plasmodium berghei Anka

Pambudi¹,

Utami²,

Budiarti³

2019

IJCTR

View full text Add to dashboard Cite

Background: Malaria is a dangerous disease that can cause death. Curcuma xanthorriza Roxb. contains curcumin as an antiinflammatory and natural antioxidant which has a hepatoprotector function. Objective: To know the effect of rhizome extract of curcuma (Curcuma xanthorriza Roxb) on cell injury in histopathology of male mice's (Mus musculus L.) liver tissue which were infected by Plasmodium berghei ANKA. Methods: The methods of the study are laboratory experimental study with the design areposttest only randomized group. Using 25 mice divided randomly into 5 groups that are: normal mice group (K1), aquadest group (K2), ginger group 150mg/kgBW (K3), ginger group 100mg/kgBW (K4), ginger group 50mg/kgBW (K5). Onthe fifth day of experiment, liver histopathology observation was performed. Results:Descriptively the results show a decrease in cell injury in cell histopathology of the liver tissue of mice. The higher the dose given the smaller the results of scoring cell injury. However, the results of the Mann-Whitney U statistical test were of significance between the groups given curcuma xanthorriza Roxb extract. 150 mg/kg body weight, 100 mg/kg body weight, 50 mg/kg body weight more than 0.05 (Sig <0.05). Conclusion: The administration of 150mg / BB extract of curcuma rhizome (Curcuma xanthorriza Roxb.) Had a descriptive effect but was not analytically significant on cell injury in histopathology of liver tissue of male mice (Plasmodium berghei ANKA).

show abstract

Section: Comparison Testmentioning

confidence: 99%

The Effect of Rhizome Extract of Curcuma (Curcuma xanthorriza Roxb) for Cell Injury in Histopathology of Liver Tissue of Male White Mice (Mus musculus L.) Strain BALB/C Infected by Plasmodium berghei Anka

Pambudi¹,

Utami²,

Budiarti³

2019

IJCTR

View full text Add to dashboard Cite

show abstract

“…Whole plant of this herbs has been tested also in 3D7 and K1 strain of P. falciparum. The result show that dichloromethane extract showed IC 50 8,42 µg/ml (3D7) and 5.85 µg/ml (K1) [85].…”

Section: Antimalarial Activitymentioning

confidence: 95%

Phytochemical screening, in vitro antimalarial activity and genetic diversity of selected asteraceae medicinal plants indigenous to Thailand

Liana¹

View full text Add to dashboard Cite

The emergence of artemisinin resistance leaded the effort to find the new antimalarial drug or artemisinin activity booster. Due to the chance that secondary metabolites can be evolutionary conserved, combining phylogeny with ethnobotanical data for screening antimalarial activity may be helpful to predict bioactivity and minimize the expenditure and time for laboratory research. The aimed of this study is screening the antimalarial activity, phytochemicals and genetic diversity of selected Asteraceae medicinal plants generated by combinatorial phylogeny and ethnobotanical data. 733 medicinal plants were obtained from literature search however only 340 taxa were met the inclusion and exclusion criteria hence these taxa were further analysis. Obtained 340 Internal Transcribed Spacer (ITS) sequences from gene bank NCBI were analyzed by MUSCLE sequence alignment and Maximum Likelihood Phylogenetic Test to generate the phylogenetic tree. Interactive Tree of Life (ITOL) was used to analyze the clustered pattern in generated phylogenetic tree. Several clades were highlighted consistently in the phylogenetic tree for malaria treatment including Asteraceae, Apocynaceae, Rubiaceae and Euphorbiaceae while the strong signal was majorly shown in Asteraceae. Afterwards, 16 ethanolic extract of Thai Asteraceae medicinal plants were investigated to determine the phytochemical screening, antimalarial activity, and the genetic diversity. Alkaloids, phenolics, flavonoids, triterpenes, steroids, saponins, diterpenes and lactones were screening by standard method. Antimalarial activity assay was done by DNA fluorescence-based assay against laboratory adapted 3D7 Plasmodium falciparum. Classification of antimalarial activity was done by categorizing the IC50 (µg/mL). In other hand, the genetic diversity was examined. Extracted plant DNA by CTAB method was amplified and sequenced by universal ITS primer. Phylogenetic analyses were performed with Cannabis sativa as outgroup species. MAFFT sequences alignment and RAxML with automatic bootstrapping were performed to generate phylogenetic tree followed by making up with ITOL and Adobe Illustrator 2020. All 16 ethanolic extract medicinal plants showed the presence of phenolics and flavonoids. Among 16 medicinal plants tested, 8 showed active which exhibited good-moderate (1; Sphaeranthus indicus), weak (4; Blumea balsamifera, Artemisia chinensis, Artemisia vulgaris, Tridax procumbens) and very weak (3; Wedelia trilobata, Eupatorium capillifolium, Vernonia cinerea) and the 8 remaining extract were showed inactive. The best promising extract is Sphaeranthus indicus with the IC50 6.59 µg/mL. Constructed phylogenetic tree using ITS region showed to be able to separate the species into their clade tribe based on current classification. In conclusion, phylogeny approach is useful to narrow down the selection of candidate taxa for bioactivity screening.

show abstract

Kinetics studies on effects of extraction techniques on bioactive compounds from Vernonia cinerea leaf

Alara

Abdurahman

2018

J Food Sci Technol

View full text Add to dashboard Cite

Recently, unconventional methods especially microwave-assisted hydrodistillation extraction (MAHE) is being used as an alternative technique for extracting bioactive compounds from plant materials due to its advantages over conventional methods such as Soxhlet extraction (SE). In this study, bioactive compounds were extracted from Vernonia cinerea leaf using both MAHE and SE methods. In addition, the kinetic study of MAHE and SE methods were carried out using first-and secondorder kinetic models. The results obtained showed that MAHE can extract higher yield of bioactive compounds from V. cinerea leaf in a shorter time and reduced used of extracting solvent compared with SE method. Based on the results obtained, second-order kinetic models can actually describe the extraction of bioactive compounds from V. cinerea leaf through MAHE with extraction rate coefficient of 0.1172 L/gmin and extraction capacity of 1.0547 L/g as compared to SE with 0.0157 L/gmin and 1.1626 L/g of extraction rate coefficient and extraction capacity, respectively. The gas chromatography-mass spectrometry analysis of the oil showed the presence of numerous heavy fractions in the oil obtained through MAHE as compared with the SE method. Moreover, the electric consumption and environmental impacts analysis of the oil suggested that MAHE can be a suitable green technique for extracting bioactive compounds from V. cinerea leaf.

show abstract

Antiplasmodial activity of Vernonia cinerea Less (Asteraceae), a plant used in traditional medicine in Burkina Faso to treat malaria

Cited by 7 publications

References 22 publications

The Effect of Rhizome Extract of Curcuma (Curcuma xanthorriza Roxb) for Cell Injury in Histopathology of Liver Tissue of Male White Mice (Mus musculus L.) Strain BALB/C Infected by Plasmodium berghei Anka

The Effect of Rhizome Extract of Curcuma (Curcuma xanthorriza Roxb) for Cell Injury in Histopathology of Liver Tissue of Male White Mice (Mus musculus L.) Strain BALB/C Infected by Plasmodium berghei Anka

Phytochemical screening, in vitro antimalarial activity and genetic diversity of selected asteraceae medicinal plants indigenous to Thailand

Kinetics studies on effects of extraction techniques on bioactive compounds from Vernonia cinerea leaf

Contact Info

Product

Resources

About